Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,884	A→G	V152V (GTT→GTC)	trmD ←	tRNA m(1)G37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,884	0	A	G	100.0%	149.2 / NA	42	V152V (GTT→GTC)	trmD	tRNA m(1)G37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

CAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATC  >  NC_000913/2744749‑2745016                                                                                                                                        |                                                                                                                                     cAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACgg                                                                                                                                  >  2:775853/1‑140 (MQ=255)   gTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAg                                                                                                                                <  1:384449/140‑1 (MQ=255)            gCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGc                                                                                                                       >  2:220033/1‑140 (MQ=255)                aaCTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCa                                                                                                                   <  1:775853/140‑1 (MQ=255)                 aCTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCAt                                                                                                                  >  1:433294/1‑140 (MQ=255)                 aCTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCAt                                                                                                                  >  1:410841/1‑140 (MQ=255)                          ccTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTaa                                                                                                         <  1:723251/140‑1 (MQ=255)                          ccTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTaa                                                                                                         <  1:652357/140‑1 (MQ=255)                                    ccTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTcaccacca                                                                                               >  1:618191/1‑140 (MQ=255)                                     cTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTcaccacca                                                                                               <  1:43820/139‑1 (MQ=255)                                     cTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTcaccacca                                                                                               >  2:43820/1‑139 (MQ=255)                                       cAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTg                                                                                            >  2:29471/1‑140 (MQ=255)                                        aGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTga                                                                                           >  2:599037/1‑140 (MQ=255)                                        aGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTga                                                                                           >  1:634868/1‑140 (MQ=255)                                        aGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTga                                                                                           >  2:138989/1‑140 (MQ=255)                                        aGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAATACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTga                                                                                           >  1:711675/1‑140 (MQ=255)                                                        gggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCg                                                                             <  1:457577/138‑1 (MQ=255)                                                        gggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCg                                                                             >  2:457577/1‑138 (MQ=255)                                                         ggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGAtt                                                                          >  2:727574/1‑140 (MQ=255)                                                            aaTCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGAc                                                                       <  1:727574/140‑1 (MQ=255)                                                                        cTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCa                                                           >  1:529459/1‑140 (MQ=255)                                                                                 aGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGtt                                                  >  1:275301/1‑140 (MQ=255)                                                                                 aGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGtt                                                  >  2:409368/1‑140 (MQ=255)                                                                                       cTTCCGTTGCCGAGGCTTCATGTCCCAATACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATc                                            <  2:711675/140‑1 (MQ=255)                                                                                          ccGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcg                                         <  1:599037/140‑1 (MQ=255)                                                                                            gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                       <  1:185938/140‑1 (MQ=255)                                                                                             ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                      <  2:433294/140‑1 (MQ=255)                                                                                             ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                      <  1:138989/140‑1 (MQ=255)                                                                                             ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                      <  2:618191/140‑1 (MQ=255)                                                                                             ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                      >  1:622953/1‑140 (MQ=255)                                                                                             ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                      <  1:220033/140‑1 (MQ=255)                                                                                             ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                      <  2:410841/140‑1 (MQ=255)                                                                                                  gAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATc                                 <  1:29471/140‑1 (MQ=255)                                                                                                        tCATGTCCCAGTACTCCCGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATAcc                           <  2:634868/140‑1 (MQ=255)                                                                                                                        ccGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCTGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac           >  2:744626/1‑140 (MQ=255)                                                                                                                        ccGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac           >  2:197171/1‑140 (MQ=255)                                                                                                                        ccGGAATAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATAGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac           >  1:654604/1‑140 (MQ=255)                                                                                                                             aTAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAg      >  1:225349/1‑140 (MQ=255)                                                                                                                             aTAAACCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAg      >  2:134673/1‑140 (MQ=255)                                                                                                                                aaCCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAAt   >  2:367536/1‑140 (MQ=255)                                                                                                                                aaCCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAAt   >  1:6080/1‑140 (MQ=255)                                                                                                                                 aCCGGGAGACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATc  <  1:134673/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATC  >  NC_000913/2744749‑2745016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑