Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 195,129 A→G N76S (AAC→AGC)  ispU → undecaprenyl diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913195,1290AG97.0% 108.0 / ‑4.4 33N76S (AAC→AGC) ispUundecaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (15/17);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTGCA  >  NC_000913/194995‑195264
                                                                                                                                      |                                                                                                                                       
ggCAAAAAAGCAAGGGAAGATTCGTGCC‑TTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGAcc                                                                                                                                   <  2:545689/140‑1 (MQ=255)
 gCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGAcc                                                                                                                                   >  2:124527/1‑140 (MQ=255)
   aaaaaaGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAg                                                                                                                                 <  2:428771/140‑1 (MQ=255)
        aGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAg                                                                                                                            <  2:729185/140‑1 (MQ=255)
               gAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCa                                                                                                                     <  2:32990/140‑1 (MQ=255)
                   aTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGc                                                                                                                 >  2:481143/1‑140 (MQ=255)
                    ttCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCg                                                                                                                >  1:360581/1‑140 (MQ=255)
                           cTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATgg                                                                                                         <  2:398566/140‑1 (MQ=255)
                           cTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATgg                                                                                                         <  2:262610/140‑1 (MQ=255)
                            tttGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGa                                                                                                        <  2:254149/140‑1 (MQ=255)
                               gggCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACt                                                                                                     <  1:124527/140‑1 (MQ=255)
                                      aaGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTtgtg                                                                                              <  2:62559/140‑1 (MQ=255)
                                          cGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTggg                                                                                          >  2:68923/1‑140 (MQ=255)
                                            gggCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGgcg                                                                                        <  2:62150/140‑1 (MQ=255)
                                                    tccgtccgCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAg                                                                                <  1:68923/140‑1 (MQ=255)
                                                       gtccgCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTtgtgt                                                                                             <  2:661146/124‑1 (MQ=255)
                                                       gtccgCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTtgtgt                                                                                             >  1:661146/1‑124 (MQ=255)
                                                                    tctcTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGTGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTg                                                                >  2:643534/1‑140 (MQ=255)
                                                                    tctcTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGt                                                                   >  2:763660/1‑137 (MQ=255)
                                                                    tctcTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGt                                                                   <  1:763660/137‑1 (MQ=255)
                                                                      tctTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCa                                                              <  1:548405/140‑1 (MQ=255)
                                                                      tctTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGCTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCa                                                              <  2:102555/140‑1 (MQ=255)
                                                                              ggCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATa                                                      >  1:530487/1‑140 (MQ=255)
                                                                              ggCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATa                                                      >  1:235758/1‑140 (MQ=255)
                                                                                 caacaaCGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACg                                                   <  1:596335/140‑1 (MQ=255)
                                                                                          tATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGc                                          >  1:602017/1‑140 (MQ=255)
                                                                                           aTTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCg                                         <  2:235758/140‑1 (MQ=255)
                                                                                                    ttAACGCTGTATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTgg                                >  1:319449/1‑140 (MQ=255)
                                                                                                             tATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAg                       <  1:617968/140‑1 (MQ=255)
                                                                                                             tATGCCTTTAGTAGTGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAg                       >  1:324003/1‑140 (MQ=255)
                                                                                                                           tGAAAACTGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTcgc         >  2:546674/1‑140 (MQ=255)
                                                                                                                                  tGGAGCCTACCAGCGCAGGAAGTCATTGCGTTAATGGAACTGTTTGTGTGGGCGATCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTGCa  >  2:319504/1‑140 (MQ=255)
                                                                                                                                  tGGAGCCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTGCa  >  2:456292/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTGCA  >  NC_000913/194995‑195264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑