Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,669	G→A	A352T (GCC→ACC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,669	0	G	A	100.0%	78.7 / NA	24	A352T (GCC→ACC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/10);  total (14/10)

TGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCGGAACGACTGG  >  NC_000913/2813545‑2813801                                                                                                                             |                                                                                                                                     tGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAAc                                                                                                                       <  1:318276/140‑1 (MQ=255) tGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAAc                                                                                                                       <  2:337632/140‑1 (MQ=255)        gTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTAt                                                                                                                >  1:751670/1‑140 (MQ=255)                  ttCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGgc                                                                                                      >  2:711193/1‑140 (MQ=255)                   tCCGGTGCTCCTGTCGCCGATTATCGACCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGgcg                                                                                                     >  2:795707/1‑140 (MQ=255)                       gTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTc                                                                                                 >  2:628422/1‑140 (MQ=255)                                tCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGcc                                                                                        <  2:767712/140‑1 (MQ=255)                                  gCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGc                                                                                      >  1:505682/1‑140 (MQ=255)                                                 cTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGt                                                                       <  1:176983/140‑1 (MQ=255)                                                 cTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGt                                                                       <  1:638035/140‑1 (MQ=255)                                                  ttCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGtt                                                                      >  2:493750/1‑140 (MQ=255)                                                         aTAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTg                                                               <  2:791370/140‑1 (MQ=255)                                                           aaaCTGGATATGCGGCGGCTGGTAGCCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAGCCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGc                                                             <  1:795707/140‑1 (MQ=255)                                                                 gATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGctt                                                       <  2:505682/140‑1 (MQ=255)                                                                           ggcTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGcttctt                                                    <  2:333178/133‑1 (MQ=255)                                                                           ggcTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGcttctt                                                    >  1:333178/1‑133 (MQ=255)                                                                                gTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGac                                        <  2:751670/140‑1 (MQ=255)                                                                                gTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTAGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGac                                        >  2:272955/1‑140 (MQ=255)                                                                                       tCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCAtt                                 >  2:266814/1‑140 (MQ=255)                                                                                                        gCCGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGcc                >  1:49974/1‑140 (MQ=255)                                                                                                          cGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCAc              >  2:227857/1‑140 (MQ=255)                                                                                                          cGTCTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCAc              >  2:571790/1‑140 (MQ=255)                                                                                                             cTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCgg           >  2:209857/1‑140 (MQ=255)                                                                                                             cTGCTTCTACTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCgg           >  1:139178/1‑140 (MQ=255)                                                                                                                      cTGGCGTACCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCGGAACGACTgg  >  2:150011/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                     TGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCGGAACGACTGG  >  NC_000913/2813545‑2813801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑