Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,534	A→G	L15L (TTG→CTG)	mltB ←	membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,534	0	A	G	100.0%	126.6 / NA	36	L15L (TTG→CTG)	mltB	membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/24);  total (12/24)

TTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACT  >  NC_000913/2825407‑2825668                                                                                                                                |                                                                                                                                       ttattaGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAgggg                                                                                                                            >  2:569760/1‑140 (MQ=255) ttattaGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAgggg                                                                                                                            >  2:23879/1‑140 (MQ=255)  tattaGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGa                                                                                                                           <  1:747171/140‑1 (MQ=255)    ttaGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAg                                                                                                                         <  1:183041/140‑1 (MQ=255)    ttaGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAg                                                                                                                         <  2:23208/140‑1 (MQ=255)    ttaGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAg                                                                                                                         <  1:618589/140‑1 (MQ=255)      aGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCa                                                                                                                       <  1:23879/140‑1 (MQ=255)         gAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATg                                                                                                                    <  2:689197/140‑1 (MQ=255)              cgccgcCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACa                                                                                                               >  1:631131/1‑140 (MQ=255)                  gccCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAac                                                                                                           <  1:203819/140‑1 (MQ=255)                   cccATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAacg                                                                                                          <  2:489222/140‑1 (MQ=255)                   cccATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAacg                                                                                                          <  1:569760/140‑1 (MQ=255)                        cTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCt                                                                                                     >  1:159141/1‑140 (MQ=255)                        cTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCt                                                                                                     <  2:151183/140‑1 (MQ=255)                                  ttGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAg                                                                                           <  1:338562/140‑1 (MQ=255)                                     tgctgcGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAgggg                                                                                        >  2:781574/1‑140 (MQ=255)                                      gctgcGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGt                                                                                       <  1:629650/140‑1 (MQ=255)                                      gctgcGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGt                                                                                       <  1:575809/140‑1 (MQ=255)                                        tgcGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTcc                                                                                     >  1:218445/1‑140 (MQ=255)                                              tCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTaa                                                                               <  1:238808/140‑1 (MQ=255)                                              tCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTaa                                                                               <  2:65579/140‑1 (MQ=255)                                               cAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAc                                                                              >  2:659033/1‑140 (MQ=255)                                               cAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTAGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGt                                                                                       <  1:365117/131‑1 (MQ=255)                                               cAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTAGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGt                                                                                       >  2:365117/1‑131 (MQ=255)                                                 aGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAg                                                                            <  2:631131/140‑1 (MQ=255)                                                         gccgccAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAAcc                                                                    <  2:159141/140‑1 (MQ=255)                                                               aGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGc                                                              <  2:70435/140‑1 (MQ=255)                                                                                tCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATc                                             <  1:781574/140‑1 (MQ=255)                                                                                tCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATc                                             <  1:61559/140‑1 (MQ=255)                                                                                 cGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCa                                            <  2:46567/140‑1 (MQ=255)                                                                                          ctcAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGca                                   <  1:247783/140‑1 (MQ=255)                                                                                            cAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGcaca                                 >  1:111873/1‑140 (MQ=255)                                                                                                                ctACAGGCGGCAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGcc             <  2:729077/140‑1 (MQ=255)                                                                                                                       cggcAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACg      >  1:627788/1‑140 (MQ=255)                                                                                                                        ggcAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTTAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACgg     >  2:458384/1‑140 (MQ=255)                                                                                                                        ggcAAGCAGCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACgg     >  2:57923/1‑140 (MQ=255)                                                                                                                          caagcagCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGAc   >  1:739012/1‑140 (MQ=255)                                                                                                                           aagcagCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCAATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACt  <  1:86403/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                       TTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACT  >  NC_000913/2825407‑2825668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑