Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,677	T→C	E196E (GAA→GAG)	ascG ←	DNA‑binding transcriptional repressor AscG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,677	0	T	C	100.0%	149.9 / NA	43	E196E (GAA→GAG)	ascG	DNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

AATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTGCG  >  NC_000913/2838543‑2838811                                                                                                                                       |                                                                                                                                       aaTTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAAt                                                                                                                                   <  1:798672/140‑1 (MQ=255) aaTTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAAt                                                                                                                                   <  1:703983/140‑1 (MQ=255)  aTTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATg                                                                                                                                  <  2:39489/140‑1 (MQ=255)     tAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCtg                                                                                                                               <  2:101990/140‑1 (MQ=255)            aCGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTggggg                                                                                                                        >  2:61466/1‑140 (MQ=255)                        cATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGc                                                                                                            >  2:507227/1‑140 (MQ=255)                            tCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGt                                                                                                        <  1:529402/140‑1 (MQ=255)                                 cccTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTTTTTATCCCCGGAAAGACGCTAAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGa                                                                                                   <  2:313001/140‑1 (MQ=255)                                  ccTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGaa                                                                                                  >  2:389732/1‑140 (MQ=255)                                  ccTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGaa                                                                                                  >  2:608777/1‑140 (MQ=255)                                   cTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGaaa                                                                                                 >  1:400488/1‑140 (MQ=255)                                    ttCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAg                                                                                                >  1:289289/1‑140 (MQ=255)                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGc                                                                                               >  1:117724/1‑140 (MQ=255)                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGc                                                                                               >  1:291481/1‑140 (MQ=255)                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGc                                                                                               >  1:392803/1‑140 (MQ=255)                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCAACGAGCCGGTAAGGAAAGc                                                                                               >  2:259676/1‑140 (MQ=255)                                                                ccGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCaa                                                                    <  2:792271/140‑1 (MQ=255)                                                                     aGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTg                                                               >  2:96724/1‑140 (MQ=255)                                                                        gATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCa                                                            <  1:692898/140‑1 (MQ=255)                                                                         aTAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCAc                                                           <  2:684157/140‑1 (MQ=255)                                                                                        aGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCTTTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGt                                            <  2:723431/140‑1 (MQ=255)                                                                                        aGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCGGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGt                                            <  1:623646/140‑1 (MQ=255)                                                                                        aGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGt                                            <  1:413911/140‑1 (MQ=255)                                                                                        aGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGt                                            <  2:392803/140‑1 (MQ=255)                                                                                        aGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGt                                            <  1:389732/140‑1 (MQ=255)                                                                                        aGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGt                                            <  1:608777/140‑1 (MQ=255)                                                                                         gcgcAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTc                                           <  1:621866/140‑1 (MQ=255)                                                                                             aaTACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGtt                                       <  2:291481/140‑1 (MQ=255)                                                                                                    tgctgcGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATc                                >  1:782245/1‑140 (MQ=255)                                                                                                    tgctgcGCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATc                                >  1:363333/1‑140 (MQ=255)                                                                                                    tgctgcCCCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCGGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATc                                <  2:787262/140‑1 (MQ=255)                                                                                                        gcgcCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCAc                            <  2:353265/140‑1 (MQ=255)                                                                                                         cgcCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCAcc                           >  1:263241/1‑140 (MQ=255)                                                                                                         cgcCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCAcc                           >  2:196379/1‑140 (MQ=255)                                                                                                         cgcCAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCAcc                           <  1:680446/140‑1 (MQ=255)                                                                                                           ccAGCGCGTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAg                         >  2:483383/1‑140 (MQ=255)                                                                                                               cgcgTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGc                     >  2:575219/1‑140 (MQ=255)                                                                                                               cgcgTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGc                     >  2:201275/1‑140 (MQ=255)                                                                                                               cgcgTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGc                     >  2:149993/1‑140 (MQ=255)                                                                                                               cgcgTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGc                     >  1:667183/1‑140 (MQ=255)                                                                                                                gcgTCTTTATACCCGGCAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCt                    <  1:575219/140‑1 (MQ=255)                                                                                                                                cAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTg    >  2:691840/1‑140 (MQ=255)                                                                                                                                cAAGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTg    >  1:517591/1‑140 (MQ=255)                                                                                                                                  aGACGCTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTgcg  <  2:363333/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTGCG  >  NC_000913/2838543‑2838811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑