Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,888	T→C	E52E (GAA→GAG)	hycI ←	hydrogenase 3 maturation protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,888	0	T	C	100.0%	147.5 / NA	43	E52E (GAA→GAG)	hycI	hydrogenase 3 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/17);  total (26/17)

AATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACA  >  NC_000913/2842758‑2843023                                                                                                                                   |                                                                                                                                        aaTTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATa                                                                                                                                >  2:112947/1‑140 (MQ=255)        cGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGcgacga                                                                                                                         >  2:124099/1‑140 (MQ=255)                        gTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgc                                                                                                         >  1:83234/1‑140 (MQ=255)                        gTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgc                                                                                                         >  1:164136/1‑140 (MQ=255)                        gTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgc                                                                                                         >  1:296963/1‑140 (MQ=255)                         tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgcg                                                                                                        >  2:363970/1‑140 (MQ=255)                         tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgcg                                                                                                        >  2:333683/1‑140 (MQ=255)                         tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgcg                                                                                                        >  2:517222/1‑140 (MQ=255)                         tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgcg                                                                                                        >  1:307849/1‑140 (MQ=255)                         tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTgcg                                                                                                        >  1:295817/1‑140 (MQ=255)                                  aCATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccgccg                                                                                               <  1:781346/140‑1 (MQ=255)                                   cATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGt                                                                                              >  2:344135/1‑140 (MQ=255)                                       tcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGGTTTCCGGTGCGCTACCGCCGTCAAt                                                                                          >  2:712995/1‑140 (MQ=255)                                       tcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTACCCCCTTATCCGTGGCGTCGACAATCAGCAGTCGGGTCGGGCGCGGCTCCCGGATAGCGACGCTGTCGTTTTCCGGTGCGCTCCCGCCGTCaac                                                                                          >  2:551005/1‑139 (MQ=255)                                           cGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcacc                                                                                      <  2:307849/140‑1 (MQ=255)                                           cGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcacc                                                                                      <  2:144326/140‑1 (MQ=255)                                            gATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcacca                                                                                     <  1:469106/140‑1 (MQ=255)                                            gATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcacca                                                                                     <  1:104195/140‑1 (MQ=255)                                              tatCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCaccacc                                                                                   >  2:689631/1‑140 (MQ=255)                                                    ccGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTa                                                                             <  1:112947/140‑1 (MQ=255)                                                        gTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCtt                                                                         >  1:427040/1‑140 (MQ=255)                                                           gatgatGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTcg                                                                      >  1:563730/1‑140 (MQ=255)                                                            atgatgCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTcgg                                                                     >  1:82098/1‑140 (MQ=255)                                                                     aTCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACCGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCCTCAATCACCACCCAGGTACCTTTCGGCgcggcggc                                                            >  2:536647/1‑140 (MQ=255)                                                                               ggTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTcc                                                  >  2:539225/1‑140 (MQ=255)                                                                                  ttAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGcc                                               <  1:363970/140‑1 (MQ=255)                                                                                    aGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCcag                                             >  1:432239/1‑140 (MQ=255)                                                                                          atatCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCgg                                       >  1:256392/1‑140 (MQ=255)                                                                                            atCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGAc                                     <  2:794688/140‑1 (MQ=255)                                                                                                 tGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcg                                >  1:396445/1‑140 (MQ=255)                                                                                                  ggCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcgc                               <  2:427040/140‑1 (MQ=255)                                                                                                  ggCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcgc                               <  1:124099/140‑1 (MQ=255)                                                                                                  ggCGTCGACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTgcgc                               >  1:486968/1‑140 (MQ=255)                                                                                                        gACAATCAGCAGTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCcatcat                         >  2:786275/1‑140 (MQ=255)                                                                                                                 cagTCGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCcctca                >  1:204733/1‑140 (MQ=255)                                                                                                                     ggtgcggggCGCGGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            <  2:204733/134‑1 (MQ=255)                                                                                                                     cGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            <  2:486968/140‑1 (MQ=255)                                                                                                                     cGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            <  1:539225/140‑1 (MQ=255)                                                                                                                     cGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            <  1:568821/140‑1 (MQ=255)                                                                                                                     cGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            <  2:563730/140‑1 (MQ=255)                                                                                                                     cGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            >  2:10046/1‑140 (MQ=255)                                                                                                                     cGTGTCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCt            <  1:689631/140‑1 (MQ=255)                                                                                                                       tgtCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTAt          >  1:474020/1‑140 (MQ=255)                                                                                                                       tgtCGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCA‑CCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTAtt         >  1:79720/1‑140 (MQ=255)                                                                                                                          cGGGCGCAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGc       <  2:568951/140‑1 (MQ=255)                                                                                                                               gcAGCTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAaca  <  1:551005/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        AATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTTCCGCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACA  >  NC_000913/2842758‑2843023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑