Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,815	T→C	T93A (ACA→GCA)	mazF ←	endoribonuclease toxin MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,815	0	T	C	100.0%	191.8 / NA	54	T93A (ACA→GCA)	mazF	endoribonuclease toxin MazF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/23);  total (31/23)

TTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACA  >  NC_000913/2910685‑2910950                                                                                                                                   |                                                                                                                                        ttGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTTACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTttctt                                                                                                                                <  1:31360/140‑1 (MQ=255) ttGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTttctt                                                                                                                                <  2:144785/140‑1 (MQ=255)  tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTttcttc                                                                                                                               >  1:604632/1‑140 (MQ=255)         tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGctc                                                                                                                        >  1:64156/1‑140 (MQ=255)                 aTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGcc                                                                                                                <  2:170834/140‑1 (MQ=255)                        gTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCa                                                                                                           <  2:710958/138‑1 (MQ=255)                        gTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCa                                                                                                           >  1:710958/1‑138 (MQ=255)                                    gAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTa                                                                                             >  1:741758/1‑140 (MQ=255)                                           gtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                                                                                      >  1:592206/1‑140 (MQ=255)                                           gtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                                                                                      >  2:265772/1‑140 (MQ=255)                                                   aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAc                                                                                            >  2:600738/1‑126 (MQ=255)                                                   aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAc                                                                                            <  1:600738/126‑1 (MQ=255)                                                   aaTAGAAGTGAG‑TAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGc                                                                             <  2:80195/140‑1 (MQ=255)                                                      aGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTa                                                                           <  1:748935/140‑1 (MQ=255)                                                        aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg                                                                         >  1:680326/1‑140 (MQ=255)                                                             aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc                                                                    >  1:697720/1‑140 (MQ=255)                                                             aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc                                                                    >  1:159871/1‑140 (MQ=255)                                                             aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc                                                                    >  1:208770/1‑140 (MQ=255)                                                                  gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGtt                                                               >  2:482730/1‑140 (MQ=255)                                                                  gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGtt                                                               >  2:681529/1‑140 (MQ=255)                                                                       acTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGc                                                                        <  2:535173/126‑1 (MQ=255)                                                                       acTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGc                                                                        >  1:535173/1‑126 (MQ=255)                                                                         tACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGAcc                                                        >  1:98656/1‑140 (MQ=255)                                                                           cccAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCgg                                                      >  2:54207/1‑140 (MQ=255)                                                                           cccAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCgg                                                      >  1:497579/1‑140 (MQ=255)                                                                           cccAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCgg                                                      >  1:268542/1‑140 (MQ=255)                                                                           cccAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCgg                                                      >  1:204665/1‑140 (MQ=255)                                                                                tCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGa                                                          <  2:103709/131‑1 (MQ=255)                                                                                tCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGa                                                          >  1:103709/1‑131 (MQ=255)                                                                                tCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATaaa                                                 >  1:44602/1‑140 (MQ=255)                                                                                tCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATaaa                                                 >  2:242466/1‑140 (MQ=255)                                                                                 cAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATaaaa                                                >  2:214346/1‑140 (MQ=255)                                                                                   gTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATaaaa                                                >  2:468837/1‑138 (MQ=255)                                                                                   gTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATaaaa                                                <  1:468837/138‑1 (MQ=255)                                                                                     aCGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAaacaac                                            >  2:126148/1‑140 (MQ=255)                                                                                     aCGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAaacaac                                            >  1:497360/1‑140 (MQ=255)                                                                                     aCGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTATTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAaacaac                                            >  2:231516/1‑140 (MQ=255)                                                                                         tAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCg                                        <  2:572191/140‑1 (MQ=255)                                                                                              ttGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACgg                                   <  1:549328/140‑1 (MQ=255)                                                                                                           gTTGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGAt                      <  1:214346/140‑1 (MQ=255)                                                                                                            ttGTAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGAtt                     <  1:425905/140‑1 (MQ=255)                                                                                                               tAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCg                  <  2:741758/140‑1 (MQ=255)                                                                                                               tAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCg                  <  2:204665/140‑1 (MQ=255)                                                                                                               tAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCg                  <  2:680326/140‑1 (MQ=255)                                                                                                               tAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCg                  <  2:98656/140‑1 (MQ=255)                                                                                                               tAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCg                  <  2:208770/140‑1 (MQ=255)                                                                                                               tAATTCCTCTGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCg                  <  1:242466/140‑1 (MQ=255)                                                                                                                      tctGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACa           >  1:351127/1‑140 (MQ=255)                                                                                                                       ctGGGGCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACaa          >  1:520588/1‑140 (MQ=255)                                                                                                                         ggggCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAgg        <  2:520588/140‑1 (MQ=255)                                                                                                                         ggggCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAgg        >  2:101056/1‑140 (MQ=255)                                                                                                                         ggggCAACTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAgg        <  1:54207/140‑1 (MQ=255)                                                                                                                               aCTGCTCCTTTCTTCGTTGCTCCTCTTGTCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAacaca  >  1:442445/1‑140 (MQ=255)                                                                                                                               aCTGCTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAacaca  <  1:510449/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACA  >  NC_000913/2910685‑2910950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑