Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,936,150	T→C	G189G (GGT→GGC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,150	0	T	C	100.0%	117.5 / NA	34	G189G (GGT→GGC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/20);  total (14/20)

GCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGG  >  NC_000913/2936019‑2936283                                                                                                                                    |                                                                                                                                      gCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCAttgt                                                                                                                               >  2:631497/1‑140 (MQ=255)            aTCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAAct                                                                                                                    <  1:163695/140‑1 (MQ=255)            aTCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAAct                                                                                                                    >  1:493384/1‑140 (MQ=255)                 ttCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCTCCGGCTCCATTGTTGATCACAACTTCttt                                                                                                               >  1:227764/1‑140 (MQ=255)                 ttCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCttt                                                                                                               <  1:541540/140‑1 (MQ=255)                    cTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGaa                                                                                                            >  1:460289/1‑140 (MQ=255)                                     aaaCTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATc                                                                                                          <  1:234564/125‑1 (MQ=255)                                     aaaCTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATc                                                                                                          >  2:234564/1‑125 (MQ=255)                                           ctgcGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCa                                                                                     <  2:651285/140‑1 (MQ=255)                                             gcgcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAgg                                                                                   <  1:598218/140‑1 (MQ=255)                                             gcgcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAgg                                                                                   <  2:68869/140‑1 (MQ=255)                                               gcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCg                                                                                 <  1:734264/140‑1 (MQ=255)                                               gcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCCGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCg                                                                                 <  2:142187/140‑1 (MQ=255)                                                  ttGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTg                                                                              <  1:625437/140‑1 (MQ=255)                                                          gcCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGAc                                                                      >  1:302718/1‑140 (MQ=255)                                                                    ccGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCT‑GGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTc                                                           >  2:631256/1‑140 (MQ=255)                                                                      gTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCg                                                          <  2:460289/140‑1 (MQ=255)                                                                        cGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGcc                                                        >  2:285092/1‑140 (MQ=255)                                                                             gCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATc                                                   >  1:216224/1‑140 (MQ=255)                                                                                   aaGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCag                                             <  2:216224/140‑1 (MQ=255)                                                                                         aaaGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAtt                                       <  1:420053/140‑1 (MQ=255)                                                                                                     cGCTGGGCGGCGTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGcc                           >  1:165680/1‑140 (MQ=255)                                                                                                              gcgTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGaa                  >  1:409067/1‑140 (MQ=255)                                                                                                               cgTTTCGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGaaa                 <  2:493384/140‑1 (MQ=255)                                                                                                                    cGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            <  1:632565/140‑1 (MQ=255)                                                                                                                    cGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            <  1:631497/140‑1 (MQ=255)                                                                                                                    cGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            <  2:357983/140‑1 (MQ=255)                                                                                                                    cGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            <  1:285092/140‑1 (MQ=255)                                                                                                                    cGATGGGTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            <  1:254094/140‑1 (MQ=255)                                                                                                                         ggTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            <  2:612976/135‑1 (MQ=255)                                                                                                                         ggTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCa            >  1:612976/1‑135 (MQ=255)                                                                                                                         ggTATCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGc       >  1:668139/1‑140 (MQ=255)                                                                                                                             tCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTgg   >  2:540958/1‑140 (MQ=255)                                                                                                                             tCGCCGGCTCCATTGTTGATCACAACTTCTTTGAATCCTGGCT‑GGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTggg  <  1:631256/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGG  >  NC_000913/2936019‑2936283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑