Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,125	T→C	E630E (GAA→GAG)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,125	0	T	C	100.0%	161.9 / NA	46	E630E (GAA→GAG)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

TTGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGC  >  NC_000913/2974002‑2974258                                                                                                                            |                                                                                                                                      ttGCAACTTATCGCGCGTCAGTTCGTTATCTGTGATGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAATGCCAGTTGCTCCACCATTTCCAGCg                                                                                                                       >  1:256393/1‑140 (MQ=255)  tGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGa                                                                                                                      <  2:371645/140‑1 (MQ=255)   gCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAACAACCAAGTGCCAGTTGCTCCACCATTTCCAGCgcc                                                                                                                     >  2:500235/1‑138 (MQ=255)   gCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGac                                                                                                                     >  2:454428/1‑140 (MQ=255)      aCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACAcc                                                                                                                  >  2:678264/1‑140 (MQ=255)          aTCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCAttt                                                                                                              >  2:802322/1‑140 (MQ=255)           tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTc                                                                                                             >  2:513861/1‑140 (MQ=255)             gcgcgTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGc                                                                                                           <  2:454496/140‑1 (MQ=255)                  tCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCa                                                                                                      >  1:46390/1‑140 (MQ=255)                           tATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCa                                                                                             >  2:339540/1‑140 (MQ=255)                                 tggtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGt                                                                                       >  2:660918/1‑140 (MQ=255)                                   gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGttt                                                                                     >  2:343641/1‑140 (MQ=255)                                       aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 <  2:578883/140‑1 (MQ=255)                                       aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 <  1:529692/140‑1 (MQ=255)                                       aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 >  2:603901/1‑140 (MQ=255)                                       aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTgcg                                                                                 >  2:63095/1‑140 (MQ=255)                                       aaaGCACCAGTGCCTCGCCTTTGCTGGCATCCCTCTTAATCGCAGTTGCATTGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCNTGNAATTTTNGCAAAGCGTTTTgcg                                                                                 <  1:500235/140‑1 (MQ=255)                                             ccAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGccc                                                                           <  1:752499/140‑1 (MQ=255)                                              cAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCt                                                                          >  1:166263/1‑140 (MQ=255)                                              cAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCt                                                                          >  1:693294/1‑140 (MQ=255)                                                gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGa                                                                        >  2:86672/1‑140 (MQ=255)                                                gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGa                                                                        >  2:172431/1‑140 (MQ=255)                                                  gCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAAt                                                                      <  1:172431/140‑1 (MQ=255)                                                     tCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTg                                                                   >  1:654908/1‑140 (MQ=255)                                                       gCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGca                                                                 <  1:660918/140‑1 (MQ=255)                                                             gCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGc                                                           >  2:584553/1‑140 (MQ=255)                                                                     cGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCg                                                   >  2:161400/1‑140 (MQ=255)                                                                             aTCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACg                                           <  1:63095/140‑1 (MQ=255)                                                                               cGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGca                                         <  1:143513/140‑1 (MQ=255)                                                                                  aGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACaa                                      >  1:285977/1‑140 (MQ=255)                                                                                    tGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAata                                    >  2:418545/1‑140 (MQ=255)                                                                                    tGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAata                                    >  2:791106/1‑140 (MQ=255)                                                                                      gCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATc                                  >  1:18657/1‑140 (MQ=255)                                                                                       cATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAG‑‑CTGCTCGTCAAAACGCACAATATCGcc                               <  1:678264/140‑1 (MQ=255)                                                                                        aTGGACTTTATCTGGCGAAACCCCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:738056/140‑1 (MQ=255)                                                                                        aTGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:166263/140‑1 (MQ=255)                                                                                        aTGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:693294/140‑1 (MQ=255)                                                                                        aTGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGc                                <  2:328554/140‑1 (MQ=255)                                                                                            aCTTTATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAgt                            <  1:339540/140‑1 (MQ=255)                                                                                                tATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCa                        >  2:616379/1‑140 (MQ=255)                                                                                                tATCTGGCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCa                        >  1:18569/1‑140 (MQ=255)                                                                                                      gCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTTGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAg                  >  2:28282/1‑140 (MQ=255)                                                                                                      gCGAAACACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAg                  <  1:529825/140‑1 (MQ=255)                                                                                                         aaaCACCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCcg               <  1:513861/140‑1 (MQ=255)                                                                                                           acacCAAGTGCCAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCcgcg             <  2:285977/140‑1 (MQ=255)                                                                                                                     ccAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcg   >  2:350816/1‑140 (MQ=255)                                                                                                                      cAGTTGCTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgc  <  1:343641/140‑1 (MQ=255)                                                                                                                        gttgctCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGcc                <  2:63078/124‑1 (MQ=255)                                                                                                                        gttgctCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGcc                >  1:63078/1‑124 (MQ=255)                                                                                                                            |                                                                                                                                      TTGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGC  >  NC_000913/2974002‑2974258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑