| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,983,421 | T→C | Q235R (CAG→CGG) | kduI ← | 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,983,421 | 0 | T | C | 96.6% | 202.8 / ‑0.4 | 59 | coding (704/837 nt) | kduI | 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (29/28); minor base . (1/1); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATGGTG > NC_000913/2983285‑2983552 | caACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCt < 2:47223/140‑1 (MQ=255) aCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACGCCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCg < 2:172679/140‑1 (MQ=255) aCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCg < 2:663993/140‑1 (MQ=255) aCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCg < 1:327037/140‑1 (MQ=255) aGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTa > 1:803458/1‑140 (MQ=255) aGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTa < 1:494538/140‑1 (MQ=255) cgcAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATg > 2:230626/1‑140 (MQ=255) cgcAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATg > 1:615218/1‑140 (MQ=255) cgcAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATg < 1:485525/140‑1 (MQ=255) aaTCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATc > 1:666613/1‑140 (MQ=255) ccACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGAcgc > 2:410560/1‑140 (MQ=255) tGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGC‑TTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCt < 1:413030/140‑1 (MQ=255) atatCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCgg < 2:581189/140‑1 (MQ=255) caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc > 1:169276/1‑140 (MQ=255) caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc > 1:751798/1‑140 (MQ=255) caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc > 1:392155/1‑140 (MQ=255) caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc > 2:224941/1‑140 (MQ=255) aaGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCcat > 2:378217/1‑140 (MQ=255) ccTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCAtg > 2:511560/1‑140 (MQ=255) ttCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGaaaa > 1:326436/1‑140 (MQ=255) cACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg < 2:659876/140‑1 (MQ=255) gACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg < 2:751798/140‑1 (MQ=255) aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg > 1:155289/1‑135 (MQ=255) aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg < 2:155289/135‑1 (MQ=255) aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg > 2:306934/1‑139 (MQ=255) aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg < 1:306934/139‑1 (MQ=255) aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGc > 2:315552/1‑140 (MQ=255) aTGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGtc < 2:666613/140‑1 (MQ=255) tGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGtca > 2:313518/1‑140 (MQ=255) ccAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTcatcat < 1:268502/140‑1 (MQ=255) ccAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTcatcat < 2:597222/140‑1 (MQ=255) ccAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTcatcat < 1:43923/140‑1 (MQ=255) cAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCatcatc < 1:559650/140‑1 (MQ=255) cAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCatcatc < 2:392155/140‑1 (MQ=255) cAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCatcatc < 2:743217/140‑1 (MQ=255) gATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCa > 2:250207/1‑140 (MQ=255) tataAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa > 1:742346/1‑140 (MQ=255) tataAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa < 2:742346/140‑1 (MQ=255) aaGCTTTTGTTCCGACACCAGAATGGATCGACCAGCTCGGGGAGATCACCGCC‑GCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaataga > 2:19046/1‑140 (MQ=255) aaGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaatag > 1:777772/1‑140 (MQ=255) aGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaataga > 1:61367/1‑140 (MQ=255) tttGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaatagaaat > 1:517974/1‑140 (MQ=255) ttGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaatagaaata < 1:250207/140‑1 (MQ=255) ggTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaaa > 1:384524/1‑140 (MQ=255) gACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCa > 1:294363/1‑140 (MQ=255) ggATCGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCg < 1:315552/140‑1 (MQ=255) cGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTggg > 1:782038/1‑140 (MQ=255) cGACCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTggg < 1:410560/140‑1 (MQ=255) aCCAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGgtg > 1:685812/1‑140 (MQ=255) ccAGCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGgtgt > 1:788232/1‑140 (MQ=255) gCTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGac < 2:61367/140‑1 (MQ=255) cTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGaca > 2:606296/1‑140 (MQ=255) cTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGaca < 1:606296/140‑1 (MQ=255) cTCGGGGAGATCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGaca < 1:748533/140‑1 (MQ=255) gggAGATCACCGCC‑GCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCa < 1:19046/140‑1 (MQ=255) agaTCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATg < 1:230626/140‑1 (MQ=255) aTCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCa > 2:637603/1‑136 (MQ=255) aTCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCa < 1:637603/136‑1 (MQ=255) aTCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATGgt < 2:803458/140‑1 (MQ=255) tCACCGCCCGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATGgtg > 1:518561/1‑140 (MQ=255) | CAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATGGTG > NC_000913/2983285‑2983552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |