Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,586	G→A	A275T (GCG→ACG)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,586	0	G	A	100.0%	151.6 / NA	44	A275T (GCG→ACG)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/17);  total (27/17)

TTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTG  >  NC_000913/3009450‑3009717                                                                                                                                         |                                                                                                                                    ttATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGgcacgc                                                                                                                                  <  2:713335/140‑1 (MQ=255)    tGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTgg                                                                                                                               >  2:351317/1‑140 (MQ=255)         aaTTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGaa                                                                                                                          >  2:571547/1‑140 (MQ=255)                    aaaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCcct                                                                                                               <  1:722625/140‑1 (MQ=255)                      aGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCcctgc                                                                                                             <  1:651450/140‑1 (MQ=255)                      aGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCcctgc                                                                                                             <  1:376553/140‑1 (MQ=255)                          aCCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCctgcctgc                                                                                                         <  2:386237/140‑1 (MQ=255)                            ccTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAg                                                                                                       >  2:684182/1‑140 (MQ=255)                                            aTCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAAcgc                                                                                       >  1:379317/1‑140 (MQ=255)                                               ttcttcACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgt                                                                                    <  2:132860/140‑1 (MQ=255)                                               ttcttcACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgt                                                                                    >  1:132860/1‑140 (MQ=255)                                                      caTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCt                                                                             <  1:294831/140‑1 (MQ=255)                                                       aTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTa                                                                            >  1:329770/1‑140 (MQ=255)                                                         ccccAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                                                                                  <  1:315676/132‑1 (MQ=255)                                                         ccccAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                                                                                  >  2:315676/1‑132 (MQ=255)                                                         ccccAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATg                                                                          >  2:151226/1‑140 (MQ=255)                                                              aGCCGTTGCGCTGTAGCAGACGGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACaa                                                                     >  2:749739/1‑140 (MQ=255)                                                                  gTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTAc                                                                 >  2:126706/1‑140 (MQ=255)                                                                        cTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGAccgt                                                           <  1:684182/140‑1 (MQ=255)                                                                        cTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGAccgt                                                           <  1:351317/140‑1 (MQ=255)                                                                           tAGCAGACAGCTGCGCAGTTTAAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGAccgtccg                                                        >  2:574406/1‑140 (MQ=255)                                                                                gACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTg                                                   >  2:175648/1‑140 (MQ=255)                                                                                   aGCTGCGCAGTTTCAATCTACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGAc                                                >  2:26160/1‑140 (MQ=255)                                                                                   aGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGAc                                                >  1:217436/1‑140 (MQ=255)                                                                                   aGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGAc                                                >  1:314666/1‑140 (MQ=255)                                                                                   aGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGAc                                                >  2:326400/1‑140 (MQ=255)                                                                                     cTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTg                                              >  1:708702/1‑140 (MQ=255)                                                                                     cTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTg                                              >  1:300339/1‑140 (MQ=255)                                                                                     cTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCCGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTg                                              >  2:151599/1‑140 (MQ=255)                                                                                       gcgcAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGc                                            <  2:211542/140‑1 (MQ=255)                                                                                                       ccGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCg                            >  1:557807/1‑140 (MQ=255)                                                                                                           cgtcTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCaa                               <  2:598473/133‑1 (MQ=255)                                                                                                           cgtcTGACCTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCaa                               >  1:598473/1‑133 (MQ=255)                                                                                                                   cTGGGGCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTcc                <  2:379317/140‑1 (MQ=255)                                                                                                                      gggCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGa             >  1:616734/1‑140 (MQ=255)                                                                                                                      gggCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGa             >  2:596920/1‑140 (MQ=255)                                                                                                                      gggCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGa             >  2:198214/1‑140 (MQ=255)                                                                                                                        gCGAAACCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGAcc           <  1:563562/140‑1 (MQ=255)                                                                                                                           aaaCCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTccc               >  1:57867/1‑133 (MQ=255)                                                                                                                           aaaCCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTccc               <  2:57867/133‑1 (MQ=255)                                                                                                                           aaaCCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTc                 <  2:27936/131‑1 (MQ=255)                                                                                                                           aaaCCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTc                 >  1:27936/1‑131 (MQ=255)                                                                                                                             aCCTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGaa      <  1:596920/140‑1 (MQ=255)                                                                                                                               cTGGGAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAg    <  1:26160/140‑1 (MQ=255)                                                                                                                                 gggAAGGCACGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTg  >  2:345718/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTG  >  NC_000913/3009450‑3009717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑