Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,010,569	T→C	V181A (GTG→GCG)	hyuA →	phenylhydantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,010,569	0	T	C	100.0%	155.6 / NA	44	V181A (GTG→GCG)	hyuA	phenylhydantoinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

GAAATCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGTGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTG  >  NC_000913/3010439‑3010705                                                                                                                                   |                                                                                                                                         gaAATCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGa                                                                                                                                 <  2:91706/140‑1 (MQ=255)   aaTCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGaaa                                                                                                                               >  2:221821/1‑140 (MQ=255)   aaTCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGaaa                                                                                                                               >  1:459053/1‑140 (MQ=255)     tCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAat                                                                                                                             >  1:261563/1‑140 (MQ=255)          atgatAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGc                                                                                                                        >  2:773783/1‑140 (MQ=255)          atgatAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGc                                                                                                                        >  2:727603/1‑140 (MQ=255)             atAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGc                                                                                                                     <  1:681251/140‑1 (MQ=255)               aGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTa                                                                                                                   <  2:296697/140‑1 (MQ=255)                               aGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAgcgggc                                                                                                   >  1:405744/1‑140 (MQ=255)                                gCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTCACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAgcgggcg                                                                                                  <  2:66044/140‑1 (MQ=255)                                    ttttAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAAt                                                                                              >  1:249985/1‑140 (MQ=255)                                    ttttAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAAt                                                                                              >  1:594746/1‑140 (MQ=255)                                              tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcgccgc                                                                                    >  1:446206/1‑140 (MQ=255)                                              tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcgccgc                                                                                    <  2:405744/140‑1 (MQ=255)                                              tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcg                                                                                        >  1:797269/1‑136 (MQ=255)                                              tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcg                                                                                        <  2:797269/136‑1 (MQ=255)                                                      cTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTaa                                                                            >  2:319120/1‑140 (MQ=255)                                                        aTCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAcc                                                                          >  2:414175/1‑140 (MQ=255)                                                        aTCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAcc                                                                          >  1:191074/1‑140 (MQ=255)                                                        aTCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCCATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAcc                                                                          >  1:95666/1‑140 (MQ=255)                                                           aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  1:727603/140‑1 (MQ=255)                                                           aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  1:809656/140‑1 (MQ=255)                                                           aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  1:85944/140‑1 (MQ=255)                                                           aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  2:446206/140‑1 (MQ=255)                                                           aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCCATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  2:95666/140‑1 (MQ=255)                                                                aaaCTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcgacgcg                                                                  >  2:746147/1‑140 (MQ=255)                                                                  aCTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCt                                                                >  2:236848/1‑140 (MQ=255)                                                                   cTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTa                                                               >  1:223999/1‑140 (MQ=255)                                                                   cTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTa                                                               >  2:658015/1‑140 (MQ=255)                                                                    tCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTAt                                                              <  1:297113/140‑1 (MQ=255)                                                                     cAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATc                                                             <  1:39010/140‑1 (MQ=255)                                                                       aCGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCAt                                                           >  2:502614/1‑140 (MQ=255)                                                                             aCGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCCATCATGCCTTg                                                     >  1:190971/1‑140 (MQ=255)                                                                               gAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAg                                                   <  1:66381/140‑1 (MQ=255)                                                                               gAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAg                                                   <  2:594746/140‑1 (MQ=255)                                                                                 ggTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTc                                                 <  1:221821/140‑1 (MQ=255)                                                                                                aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCGCCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  <  2:459053/140‑1 (MQ=255)                                                                                                aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  <  2:261563/140‑1 (MQ=255)                                                                                                aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  <  1:319120/140‑1 (MQ=255)                                                                                                aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  >  2:124419/1‑140 (MQ=255)                                                                                                      cTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCgg                            <  1:785328/140‑1 (MQ=255)                                                                                                       ttGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGa                           >  2:726173/1‑140 (MQ=255)                                                                                                       ttGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGa                           <  1:718112/140‑1 (MQ=255)                                                                                                                                ccGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTg  <  1:122879/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GAAATCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGTGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTG  >  NC_000913/3010439‑3010705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑