Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,124,790	C→T	A224T (GCA→ACA)	glcF ←	glycolate dehydrogenase, putative iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,124,790	0	C	T	100.0%	127.2 / NA	38	A224T (GCA→ACA)	glcF	glycolate dehydrogenase, putative iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/20);  total (18/20)

GGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGC  >  NC_000913/3124653‑3124926                                                                                                                                          |                                                                                                                                         ggCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCa                                                                                                                                        >  2:34799/1‑140 (MQ=255)     tataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGccc                                                                                                                                    >  2:770974/1‑140 (MQ=255)            cATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTcc                                                                                                                             <  1:16185/140‑1 (MQ=255)             aTCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCt                                                                                                                            <  2:245873/140‑1 (MQ=255)               cGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTgc                                                                                                                          >  1:565199/1‑140 (MQ=255)                 tttttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTgcgc                                                                                                                        <  2:565199/140‑1 (MQ=255)                 tttttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTgcgc                                                                                                                        >  1:642690/1‑140 (MQ=255)                 tttttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTgcgc                                                                                                                        <  1:770974/140‑1 (MQ=255)                       aGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAg                                                                                                                  >  2:622465/1‑140 (MQ=255)                         cATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAgat                                                                                                                <  2:108057/140‑1 (MQ=255)                           tCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAgatga                                                                                                              >  1:520907/1‑140 (MQ=255)                                   tACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCAc                                                                                                      <  2:198992/140‑1 (MQ=255)                                       ctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACcgcg                                                                                                  <  2:70190/140‑1 (MQ=255)                                         ttGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAga                                                                                                                 >  2:729421/1‑123 (MQ=255)                                         ttGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAga                                                                                                                 <  1:729421/123‑1 (MQ=255)                                         ttGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGcc                                                                                                >  2:79563/1‑140 (MQ=255)                                          tGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCca                                                                                               >  2:613821/1‑140 (MQ=255)                                                 cgcgCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGc                                                                                        <  2:642690/140‑1 (MQ=255)                                                          gCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGt                                                                               >  1:778060/1‑140 (MQ=255)                                                                ggCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTg                                                                         >  2:334590/1‑140 (MQ=255)                                                                     tttGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATg                                                                    >  1:59784/1‑140 (MQ=255)                                                                          aaaaTTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATGGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCGCCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCt                                                               <  2:358690/140‑1 (MQ=255)                                                                                   tCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAg                                                      >  2:576120/1‑140 (MQ=255)                                                                                    cGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGa                                                     <  1:79563/140‑1 (MQ=255)                                                                                                    cgcgGGCCACCAGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTcgcg                                     <  1:783295/140‑1 (MQ=255)                                                                                                          ccaccaGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCACAGACGATCCAGCACTCGCGCAGTTg                               <  2:735320/140‑1 (MQ=255)                                                                                                           caccaGGCATCAATATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGc                              <  1:334590/140‑1 (MQ=255)                                                                                                                      aaTATTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGgtgt                   <  2:772817/140‑1 (MQ=255)                                                                                                                      aaTACTATTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGgtgt                   <  2:389273/140‑1 (MQ=255)                                                                                                                        tattatTGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTg                 <  2:32205/140‑1 (MQ=255)                                                                                                                            attGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCg             <  2:35912/140‑1 (MQ=255)                                                                                                                            attGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCg             <  2:520907/140‑1 (MQ=255)                                                                                                                            attGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCg             <  1:622465/140‑1 (MQ=255)                                                                                                                            attGCGCGCCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCg             <  2:778060/140‑1 (MQ=255)                                                                                                                                cgcgcCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAg         >  1:551186/1‑140 (MQ=255)                                                                                                                                  cgcCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCg       >  2:275230/1‑140 (MQ=255)                                                                                                                                  cgcCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCg       >  1:445510/1‑140 (MQ=255)                                                                                                                                  cgcCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCg       >  1:231281/1‑140 (MQ=255)                                                                                                                                  cgcCCGTGTCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCg       >  1:102593/1‑140 (MQ=255)                                                                                                                                       gtgtCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGc  <  1:613821/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGC  >  NC_000913/3124653‑3124926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑