Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,127,983	T→C	L13L (TTA→TTG)	glcD ←	glycolate dehydrogenase, putative FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,127,983	0	T	C	100.0%	183.5 / NA	51	L13L (TTA→TTG)	glcD	glycolate dehydrogenase, putative FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

AATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCT  >  NC_000913/3127852‑3128116                                                                                                                                    |                                                                                                                                      aaTGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGcc                                                                                                                               <  1:48171/140‑1 (MQ=255)       cgcgTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAg                                                                                                                         >  2:793999/1‑140 (MQ=255)       cgcgTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAg                                                                                                                         >  2:513116/1‑140 (MQ=255)       cgcgTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAg                                                                                                                         >  2:167435/1‑140 (MQ=255)         cgTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGAc                                                                                                                       <  2:556676/140‑1 (MQ=255)         cgTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGAc                                                                                                                       <  1:265513/140‑1 (MQ=255)          gTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACg                                                                                                                      >  2:49254/1‑140 (MQ=255)          gTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACg                                                                                                                      >  1:776455/1‑140 (MQ=255)            gCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTATCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGct                                                                                                                    <  2:367050/140‑1 (MQ=255)            gCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGct                                                                                                                    <  1:167435/140‑1 (MQ=255)            gCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGct                                                                                                                    <  2:803970/140‑1 (MQ=255)                  cgcgCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGt                                                                                                              >  2:33784/1‑140 (MQ=255)                   gcgcTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTa                                                                                                             <  1:568912/140‑1 (MQ=255)                       tCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAg                                                                                                         >  2:554547/1‑140 (MQ=255)                        cAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGa                                                                                                        <  2:776455/140‑1 (MQ=255)                          aCCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATg                                                                                                      <  2:98189/140‑1 (MQ=255)                             cGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTc                                                                                                   >  1:558300/1‑140 (MQ=255)                                 acacTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGa                                                                                               >  1:683661/1‑140 (MQ=255)                                  cacTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAg                                                                                              <  2:558300/140‑1 (MQ=255)                                      cGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAgg                                                                                          <  1:49254/140‑1 (MQ=255)                                         aaGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCtt                                                                                       >  2:662659/1‑140 (MQ=255)                                           ggAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCg                                                                                     <  1:169507/140‑1 (MQ=255)                                           ggAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCg                                                                                     <  1:358724/140‑1 (MQ=255)                                           ggAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCg                                                                                     <  1:307927/140‑1 (MQ=255)                                               tGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCttt                                                                                 >  2:530677/1‑140 (MQ=255)                                                   ctcctcATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTttgttg                                                                             <  1:785358/140‑1 (MQ=255)                                                      ctcATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTtgttgtgt                                                                          >  1:741740/1‑140 (MQ=255)                                                           cGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTtgtgttgtgt                                                                     >  1:740996/1‑140 (MQ=255)                                                           cGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTtgtgttgtgt                                                                     <  1:554547/140‑1 (MQ=255)                                                              tATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGc                                                                  >  1:744304/1‑140 (MQ=255)                                                                          cAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTg                                                      <  2:740996/140‑1 (MQ=255)                                                                                   ggACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCgctgct                                             >  2:321128/1‑140 (MQ=255)                                                                                        tGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgt                                        >  1:634372/1‑140 (MQ=255)                                                                                           tCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                     <  2:741740/140‑1 (MQ=255)                                                                                           tCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                     <  1:33784/140‑1 (MQ=255)                                                                                           tCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                     <  1:793999/140‑1 (MQ=255)                                                                                              cGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAg                                  >  1:15608/1‑140 (MQ=255)                                                                                                  gTGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGttat                              >  1:181741/1‑140 (MQ=255)                                                                                                   tGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGttatt                             >  1:214121/1‑140 (MQ=255)                                                                                                   tGCCATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGttatt                             >  1:699673/1‑140 (MQ=255)                                                                                                     ccATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                     >  2:522852/1‑130 (MQ=255)                                                                                                     ccATCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                     <  1:522852/130‑1 (MQ=255)                                                                                                       aTCAGTACCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGc                         >  2:464755/1‑140 (MQ=255)                                                                                                             aCCGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCtt                   >  2:225968/1‑140 (MQ=255)                                                                                                               cGATGTGCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCtttt                 >  2:667089/1‑140 (MQ=255)                                                                                                                    tgCGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTATGTGGCAGCAGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttt            >  2:725163/1‑140 (MQ=255)                                                                                                                      cGGTCGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttttc          >  1:770137/1‑140 (MQ=255)                                                                                                                          cGACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCt      <  1:321128/140‑1 (MQ=255)                                                                                                                           gACATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGATGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTa     <  1:662659/140‑1 (MQ=255)                                                                                                                             cATCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTAtc   <  1:464755/140‑1 (MQ=255)                                                                                                                              aTCGGGCAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTAtct  >  2:738485/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      AATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCT  >  NC_000913/3127852‑3128116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑