Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,157,434	A→G	D271G (GAC→GGC)	dkgA →	methylglyoxal reductase DkgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,434	0	A	G	100.0%	117.3 / NA	34	D271G (GAC→GGC)	dkgA	methylglyoxal reductase DkgA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/20);  total (14/20)

CTGGTGGTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACA  >  NC_000913/3157298‑3157570                                                                                                                                         |                                                                                                                                         cTGGTGGTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCa                                                                                                                                       >  1:709856/1‑140 (MQ=255)  tggtggTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAg                                                                                                                                      >  1:278598/1‑140 (MQ=255)                      gTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCaaa                                                                                                                  >  2:185076/1‑140 (MQ=255)                          cacCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATtct                                                                                                              <  1:802409/140‑1 (MQ=255)                                 aCGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTg                                                                                                       >  2:783822/1‑140 (MQ=255)                                  cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTgg                                                                                                      <  1:74766/140‑1 (MQ=255)                                     aTTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCgg                                                                                                   >  1:485509/1‑140 (MQ=255)                                             aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                           <  2:485509/140‑1 (MQ=255)                                             aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                           <  1:185076/140‑1 (MQ=255)                                             aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                           <  1:783822/140‑1 (MQ=255)                                                         cTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACt                                                                               <  2:278598/140‑1 (MQ=255)                                                                 tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                       <  1:197276/140‑1 (MQ=255)                                                                   cGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAAt                                                                     <  2:2226/140‑1 (MQ=255)                                                                                   aaCTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTAtt                                                             <  2:186916/132‑1 (MQ=255)                                                                                   aaCTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTAtt                                                             >  1:186916/1‑132 (MQ=255)                                                                                   aaCTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTcgc                                                     >  1:684600/1‑140 (MQ=255)                                                                                   aaCTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTcgc                                                     >  1:64095/1‑140 (MQ=255)                                                                                         gCGAAATTGCAAAACTCGATCAGGGCTAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGCCTTTTCAGAAATCATTTATTCCCCTCGCGTcccg                                               <  2:718253/140‑1 (MQ=255)                                                                                          cGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTcccgc                                              >  1:629256/1‑140 (MQ=255)                                                                                           gAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTcccgcc                                             <  2:365603/140‑1 (MQ=255)                                                                                             aaTTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTcccg                                               <  1:122501/136‑1 (MQ=255)                                                                                             aaTTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTcccg                                               >  2:122501/1‑136 (MQ=255)                                                                                                  cAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCgttgtt                                      <  1:504122/140‑1 (MQ=255)                                                                                                     aaCTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTAct                                   <  2:629256/140‑1 (MQ=255)                                                                                                          gATCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTATCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTcc                              <  2:709856/140‑1 (MQ=255)                                                                                                            tCAGGGCAAGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCtt                            >  1:231138/1‑140 (MQ=255)                                                                                                                    aGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                    >  2:222905/1‑140 (MQ=255)                                                                                                                    aGCGTCTCGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                    <  1:57252/140‑1 (MQ=255)                                                                                                                         ctcGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGcc               <  2:64095/140‑1 (MQ=255)                                                                                                                          tcGGTCCCGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa              >  2:795975/1‑140 (MQ=255)                                                                                                                                ccGATCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAAtata        <  2:266261/140‑1 (MQ=255)                                                                                                                                   aTCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAGATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAgg     >  2:703562/1‑140 (MQ=255)                                                                                                                                    tCCTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa    <  1:795975/140‑1 (MQ=255)                                                                                                                                      cTGGCCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGAca  <  1:173824/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGGTGGTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACA  >  NC_000913/3157298‑3157570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑