Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,008	T→C	Y221C (TAC→TGC)	ftsP ←	cell division protein required during stress conditions

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,008	0	T	C	100.0%	210.3 / NA	59	Y221C (TAC→TGC)	ftsP	cell division protein required during stress conditions
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

ACAGGAGCAGGCAGGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAA  >  NC_000913/3161873‑3162142                                                                                                                                        |                                                                                                                                       aCAGGAGCAGGCAGGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATATCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACgg                                                                                                                                    <  1:708091/140‑1 (MQ=255)     gAGCAGGCAGGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCTAGCCACGCGAGACTTCAACGCACGGGCtt                                                                                                                                >  2:697087/1‑140 (MQ=255)       gcaggcagGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTg                                                                                                                              >  1:208279/1‑140 (MQ=255)         aggcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTa                                                                                                                            >  2:578416/1‑140 (MQ=255)         aggcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTa                                                                                                                            <  1:489325/140‑1 (MQ=255)         aggcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAAACTTCAACGCACGGGCTTTGTa                                                                                                                            <  1:576847/140‑1 (MQ=255)          ggcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTac                                                                                                                           >  1:52060/1‑140 (MQ=255)          ggcaggAATCCCTGACCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTac                                                                                                                           >  1:784747/1‑140 (MQ=255)           gcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTaca                                                                                                                          >  1:80614/1‑140 (MQ=255)            caggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTacac                                                                                                                         <  2:784260/140‑1 (MQ=255)            caggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTacac                                                                                                                         <  2:483270/140‑1 (MQ=255)            caggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTacac                                                                                                                         <  2:320476/140‑1 (MQ=255)               gAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGt                                                                                                                      >  1:82415/1‑140 (MQ=255)                      tGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACcag                                                                                                               <  1:611679/140‑1 (MQ=255)                       gATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACcagc                                                                                                              >  1:597611/1‑140 (MQ=255)                         tCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACcagcag                                                                                                            >  2:581216/1‑140 (MQ=255)                         tCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACcagcag                                                                                                            >  1:362786/1‑140 (MQ=255)                          cGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCagcagc                                                                                                           >  1:581843/1‑140 (MQ=255)                           gCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCg                                                                                                          <  2:52060/140‑1 (MQ=255)                            ccAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGt                                                                                                         >  2:639720/1‑140 (MQ=255)                              aGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTAt                                                                                                       >  2:200065/1‑140 (MQ=255)                              aGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTAt                                                                                                       >  1:264725/1‑140 (MQ=255)                               gAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCACACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATc                                                                                                      <  2:602215/140‑1 (MQ=255)                                             cGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAgccgc                                                                                        <  2:362786/140‑1 (MQ=255)                                              gggCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAgccgcc                                                                                       >  2:520604/1‑140 (MQ=255)                                              gggCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAgccgcc                                                                                       <  1:53154/140‑1 (MQ=255)                                                   aCCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTc                                                                                  >  1:728054/1‑140 (MQ=255)                                                      aTCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGTCGCGTTCAGCAGTCGCAAGAGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCg                                                                               <  2:558338/140‑1 (MQ=255)                                                      aTCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCg                                                                               >  2:17002/1‑140 (MQ=255)                                                        cGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGt                                                                             <  1:17002/140‑1 (MQ=255)                                                         gTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGtt                                                                            <  2:728054/140‑1 (MQ=255)                                                             aTCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGtt                                                                        <  2:581843/140‑1 (MQ=255)                                                                  tAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTAtt                                                                   <  1:639720/140‑1 (MQ=255)                                                                     ttGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTg                                                                >  1:565743/1‑140 (MQ=255)                                                                        aTAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCg                                                             >  2:374043/1‑140 (MQ=255)                                                                         tAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGt                                                            >  2:62427/1‑140 (MQ=255)                                                                          aGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTa                                                           >  1:7071/1‑140 (MQ=255)                                                                            cgacgaGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTAcc                                                         >  2:387153/1‑140 (MQ=255)                                                                              acgaGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTAc                                                          <  1:662435/137‑1 (MQ=255)                                                                              acgaGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTAc                                                          >  2:662435/1‑137 (MQ=255)                                                                                     ttCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATc                                                >  1:175327/1‑140 (MQ=255)                                                                                      tCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATcc                                               <  2:261658/140‑1 (MQ=255)                                                                                        gACGCGTTCAGCAGTCGCAAGCGTACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAg                                             <  1:736570/140‑1 (MQ=255)                                                                                            cgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGt                                         <  2:208279/140‑1 (MQ=255)                                                                                             gTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGtt                                        >  1:426442/1‑140 (MQ=255)                                                                                                cagcagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTAt                                     <  2:264725/140‑1 (MQ=255)                                                                                                cagcagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTAt                                     <  1:581216/140‑1 (MQ=255)                                                                                                cagcagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTAt                                     <  1:524985/140‑1 (MQ=255)                                                                                                          aaGCGCACCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAAt                           <  1:520604/140‑1 (MQ=255)                                                                                                                aCCCAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCgg                     >  2:261512/1‑140 (MQ=255)                                                                                                                   cAGCCACGCGAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGaaa                  <  1:387153/140‑1 (MQ=255)                                                                                                                         cgcgAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAatcatc            >  1:400511/1‑140 (MQ=255)                                                                                                                          gcgAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATcc           <  1:303998/140‑1 (MQ=255)                                                                                                                           cgAGACTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCca          <  1:62427/140‑1 (MQ=255)                                                                                                                                cTTCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCa     >  1:273878/1‑140 (MQ=255)                                                                                                                                 ttCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCAt    <  2:273878/140‑1 (MQ=255)                                                                                                                                  tCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATa   <  2:400511/140‑1 (MQ=255)                                                                                                                                  tCAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATa   <  2:175327/140‑1 (MQ=255)                                                                                                                                   cAACGCACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATaa  <  2:139502/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ACAGGAGCAGGCAGGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAA  >  NC_000913/3161873‑3162142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑