Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,507	A→G	G109G (GGT→GGC)	cpdA ←	cAMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,507	0	A	G	100.0%	148.5 / NA	42	G109G (GGT→GGC)	cpdA	cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/24);  total (18/24)

GCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTA  >  NC_000913/3176371‑3176640                                                                                                                                         |                                                                                                                                      gcgcATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGccc                                                                                                                                    <  2:620080/140‑1 (MQ=255)    cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCa                                                                                                                                 <  1:87528/140‑1 (MQ=255)    cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCa                                                                                                                                 <  2:675467/140‑1 (MQ=255)           cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTa                                                                                                                          <  1:611664/140‑1 (MQ=255)             gTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAAc                                                                                                                        <  2:131831/140‑1 (MQ=255)              tttACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAAcg                                                                                                                       <  2:447199/140‑1 (MQ=255)              tttACGGTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAAcg                                                                                                                       <  2:544804/140‑1 (MQ=255)                tACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACATGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAAcgcg                                                                                                                     <  2:553270/140‑1 (MQ=255)                tACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAAcgcg                                                                                                                     <  1:541357/140‑1 (MQ=255)                        aGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACAt                                                                                                             >  2:654646/1‑140 (MQ=255)                                     gAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGaaa                                                                                                <  1:508657/140‑1 (MQ=255)                                            gctcagctcaCCGTGCGGCACACCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGt                                                                                         >  2:154739/1‑140 (MQ=255)                                                        gTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGcc                                                                             >  1:312483/1‑140 (MQ=255)                                                            ggCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGAc                                                                         >  2:648683/1‑140 (MQ=255)                                                                        acCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACg                                                             <  2:340594/140‑1 (MQ=255)                                                                                  ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCg                                                   >  2:511510/1‑140 (MQ=255)                                                                                      cAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGc                                               >  1:196578/1‑140 (MQ=255)                                                                                         cAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCtt                                            >  1:161671/1‑140 (MQ=255)                                                                                              tttGCCATTGCTCACCTATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCg                                       <  2:727522/140‑1 (MQ=255)                                                                                               ttGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGa                                      <  1:451518/140‑1 (MQ=255)                                                                                               ttGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGa                                      >  1:372119/1‑140 (MQ=255)                                                                                               ttGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGa                                      <  2:312483/140‑1 (MQ=255)                                                                                                           aCCAATAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAgg                          <  2:196578/140‑1 (MQ=255)                                                                                                              aaTAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCg                       <  1:154739/140‑1 (MQ=255)                                                                                                                tAAACACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCa                     >  2:167302/1‑140 (MQ=255)                                                                                                                 aaaCACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCag                    <  2:155438/140‑1 (MQ=255)                                                                                                                  aaCACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAgg                          <  1:57361/133‑1 (MQ=255)                                                                                                                  aaCACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAgg                          >  2:57361/1‑133 (MQ=255)                                                                                                                  aaCACGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCaga                   >  1:268603/1‑140 (MQ=255)                                                                                                                     acGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCag                    <  1:659602/136‑1 (MQ=255)                                                                                                                     acGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCag                    >  2:659602/1‑136 (MQ=255)                                                                                                                     acGCGCTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGa                >  1:141346/1‑140 (MQ=255)                                                                                                                       gcgcTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGAtt              >  1:599288/1‑140 (MQ=255)                                                                                                                        cgcTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCg                       <  2:533749/130‑1 (MQ=255)                                                                                                                        cgcTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCg                       >  1:533749/1‑130 (MQ=255)                                                                                                                          cTTCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGAt           >  1:418089/1‑140 (MQ=255)                                                                                                                           ttCGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATc          <  2:268603/140‑1 (MQ=255)                                                                                                                             cGCCGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCt        >  1:468598/1‑140 (MQ=255)                                                                                                                               ccGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTgc      <  1:654646/140‑1 (MQ=255)                                                                                                                                cGGGGAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTgcg     <  2:580936/140‑1 (MQ=255)                                                                                                                                 ggggAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTgcgc    <  2:141346/140‑1 (MQ=255)                                                                                                                                   ggAGATGCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTa  >  2:735213/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTA  >  NC_000913/3176371‑3176640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑