Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,512	G→A	G337D (GGC→GAC)	proA →	glutamate‑5‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,512	0	G	A	100.0%	137.4 / NA	40	G337D (GGC→GAC)	proA	glutamate‑5‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/16);  total (24/16)

GGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGC  >  NC_000913/262375‑262647                                                                                                                                          |                                                                                                                                        ggCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGAca                                                                                                                                       <  2:395671/140‑1 (MQ=255)  gcgcAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGacac                                                                                                                                      >  1:342913/1‑140 (MQ=255)       gTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACacaac                                                                                                                                 >  1:580164/1‑140 (MQ=255)            aggcaggcCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTcc                                                                                                                            >  1:62108/1‑140 (MQ=255)                  gcCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCg                                                                                                                      >  1:415492/1‑140 (MQ=255)                     cTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATc                                                                                                                   <  1:662855/140‑1 (MQ=255)                          aaGGTGGTTGCTGTTACAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGAc                                                                                                              <  2:727032/140‑1 (MQ=255)                          aaGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGAc                                                                                                              <  2:691873/140‑1 (MQ=255)                            ggtggtTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGAccc                                                                                                            <  2:415492/140‑1 (MQ=255)                                  tGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGata                                                                                                      >  2:237899/1‑140 (MQ=255)                                        tAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCa                                                                                                <  2:642773/140‑1 (MQ=255)                                           aGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACg                                                                                             >  1:62504/1‑140 (MQ=255)                                                aaGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAg                                                                                        >  2:340983/1‑140 (MQ=255)                                                 agagTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGc                                                                                       >  2:571211/1‑140 (MQ=255)                                                           gATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTaa                                                                             <  2:402174/140‑1 (MQ=255)                                                             tGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTaa                                                                             >  2:754554/1‑138 (MQ=255)                                                             tGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTaa                                                                             <  1:754554/138‑1 (MQ=255)                                                             tGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACg                                                                           >  2:524435/1‑140 (MQ=255)                                                               aGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGaa                                                                         >  1:603944/1‑140 (MQ=255)                                                                   tCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTgg                                                                     >  1:436722/1‑140 (MQ=255)                                                                         aTTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGt                                                               >  2:734658/1‑140 (MQ=255)                                                                           tAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTcc                                                             <  1:33372/140‑1 (MQ=255)                                                                           tAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTcc                                                             <  2:436722/140‑1 (MQ=255)                                                                            aGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCg                                                            <  2:376584/140‑1 (MQ=255)                                                                            aGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCg                                                            <  2:499867/140‑1 (MQ=255)                                                                                   aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCg                                                            >  2:17925/1‑133 (MQ=255)                                                                                   aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCg                                                            <  1:17925/133‑1 (MQ=255)                                                                                   aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTa                                                     >  2:85107/1‑140 (MQ=255)                                                                                                tCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCctct                                        <  1:524435/140‑1 (MQ=255)                                                                                                   gCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTAcg                                     >  2:234122/1‑140 (MQ=255)                                                                                                             cGATGCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCg                           >  1:138203/1‑140 (MQ=255)                                                                                                                 gCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcgg                       >  1:532229/1‑140 (MQ=255)                                                                                                                 gCCATCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcgg                       >  2:776247/1‑140 (MQ=255)                                                                                                                     tCGCCCATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACggcggc                   <  2:749718/140‑1 (MQ=255)                                                                                                                          cATATTCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGtt              >  1:790714/1‑140 (MQ=255)                                                                                                                              ttCGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt          <  2:62504/140‑1 (MQ=255)                                                                                                                                cGTGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCt        >  2:688555/1‑140 (MQ=255)                                                                                                                                  tGAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTgg      >  2:435687/1‑140 (MQ=255)                                                                                                                                   gAACACGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTggg     >  1:148887/1‑140 (MQ=255)                                                                                                                                      cacGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGc  >  2:420152/1‑140 (MQ=255)                                                                                                                                      cacGACACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGc  >  2:25139/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGC  >  NC_000913/262375‑262647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑