Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,396	A→G	T428A (ACG→GCG)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,396	0	A	G	100.0%	206.2 / NA	58	T428A (ACG→GCG)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/29);  total (29/29)

CTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACC  >  NC_000913/3179263‑3179532                                                                                                                                      |                                                                                                                                         cTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGa                                                                                                                                    >  2:553027/1‑140 (MQ=255)  tACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGaa                                                                                                                                   <  2:95386/140‑1 (MQ=255)      cggtcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAg                                                                                                                               <  2:377888/140‑1 (MQ=255)      cggtcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAg                                                                                                                               <  1:324163/140‑1 (MQ=255)         tcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAg                                                                                                                            <  1:736354/140‑1 (MQ=255)                       aTTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACt                                                                                                              <  1:734752/140‑1 (MQ=255)                       aTTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACt                                                                                                              >  1:427946/1‑140 (MQ=255)                          gttgATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGaa                                                                                                           >  2:438740/1‑140 (MQ=255)                                gtgtTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAAt                                                                                                       <  1:674299/138‑1 (MQ=255)                                gtgtTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAAt                                                                                                       >  2:674299/1‑138 (MQ=255)                                gtgtTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATgc                                                                                                     >  2:255227/1‑140 (MQ=255)                                 tgtTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATgcg                                                                                                    <  1:38339/140‑1 (MQ=255)                                   ttGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCt                                                                                                  >  1:123575/1‑140 (MQ=255)                                   ttGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCt                                                                                                  >  1:276922/1‑140 (MQ=255)                                   ttGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCCGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCt                                                                                                  >  1:449433/1‑140 (MQ=255)                                        tGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCa                                                                                             >  2:235944/1‑140 (MQ=255)                                           gACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCCGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAAc                                                                                          <  2:449433/140‑1 (MQ=255)                                             ccaccaCGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCg                                                                                        <  1:275108/140‑1 (MQ=255)                                               accacGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGt                                                                                      <  1:301215/140‑1 (MQ=255)                                                    gTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCa                                                                                 <  1:523260/140‑1 (MQ=255)                                                       gTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTa                                                                              <  2:123575/140‑1 (MQ=255)                                                           aaCGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATcc                                                                          >  1:564326/1‑140 (MQ=255)                                                           aaCGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATcc                                                                          >  1:645557/1‑140 (MQ=255)                                                             cGCCAAGCGAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCgg                                                                        >  2:262167/1‑140 (MQ=255)                                                             cGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCgg                                                                        >  2:488573/1‑140 (MQ=255)                                                              gCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGa                                                                       >  1:23680/1‑140 (MQ=255)                                                                  agcaagAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTG‑ATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACg                                                                  >  1:279126/1‑140 (MQ=255)                                                                             gCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCa                                                        <  1:438740/140‑1 (MQ=255)                                                                             gCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCa                                                        <  1:290044/140‑1 (MQ=255)                                                                             gCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCa                                                        >  1:249891/1‑140 (MQ=255)                                                                             gCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCa                                                        >  2:290044/1‑140 (MQ=255)                                                                               gAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCaaa                                                      <  2:276922/140‑1 (MQ=255)                                                                               gAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCaaa                                                      <  2:564326/140‑1 (MQ=255)                                                                                         tataACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACa                                            <  1:16898/140‑1 (MQ=255)                                                                                          ataACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAg                                           <  1:232772/140‑1 (MQ=255)                                                                                            aaCTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGaa                                         <  2:177397/140‑1 (MQ=255)                                                                                            aaCTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGaa                                         <  2:283135/140‑1 (MQ=255)                                                                                                 ccTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTa                                    >  2:298583/1‑140 (MQ=255)                                                                                                     aTTAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGc                                >  1:802068/1‑140 (MQ=255)                                                                                                      ttAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCt                               >  2:519960/1‑140 (MQ=255)                                                                                                      ttAATCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCt                               <  1:586570/140‑1 (MQ=255)                                                                                                        aaTCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGa                             <  1:262167/140‑1 (MQ=255)                                                                                                          tCAGCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATg                           >  2:703154/1‑140 (MQ=255)                                                                                                             gCTGAATATTAAGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGtt                        <  2:249891/140‑1 (MQ=255)                                                                                                                       aaGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGa              <  2:111736/140‑1 (MQ=255)                                                                                                                       aaGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGa              <  2:803205/140‑1 (MQ=255)                                                                                                                        aGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGAt             <  1:31886/140‑1 (MQ=255)                                                                                                                        aGTCAGCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGAt             <  2:279126/140‑1 (MQ=255)                                                                                                                             gCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATgc                    <  1:299594/128‑1 (MQ=255)                                                                                                                             gCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATgc                    >  2:299594/1‑128 (MQ=255)                                                                                                                             gCTCTGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGccc        >  2:36925/1‑140 (MQ=255)                                                                                                                              ctctGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCg       >  2:465675/1‑140 (MQ=255)                                                                                                                              ctctGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCg       >  1:310316/1‑140 (MQ=255)                                                                                                                              ctctGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCg       >  1:65284/1‑140 (MQ=255)                                                                                                                              ctctGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCg       >  2:343610/1‑140 (MQ=255)                                                                                                                              ctctGGGTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCg       >  2:720158/1‑140 (MQ=255)                                                                                                                                  gggTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCAc   <  1:298583/140‑1 (MQ=255)                                                                                                                                   ggTGCGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCAcc  >  2:61128/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACC  >  NC_000913/3179263‑3179532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑