Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,221,241	T→C	T3A (ACC→GCC)	ygjH ←	putative tRNA‑binding protein YgjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,221,241	0	T	C	100.0%	160.2 / NA	45	T3A (ACC→GCC)	ygjH	putative tRNA‑binding protein YgjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/23);  total (22/23)

CTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCA  >  NC_000913/3221105‑3221373                                                                                                                                         |                                                                                                                                     cTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTc                                                                                                                                   <  1:39442/140‑1 (MQ=255)    gTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCAt                                                                                                                                >  1:711385/1‑140 (MQ=255)      cACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTg                                                                                                                              <  1:7247/140‑1 (MQ=255)       aCGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGc                                                                                                                             <  1:643381/140‑1 (MQ=255)          gTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTtc                                                                                                                          <  2:549036/140‑1 (MQ=255)           tttGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTtcc                                                                                                                         <  1:473268/140‑1 (MQ=255)              gCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTtcctcc                                                                                                                      >  2:349290/1‑140 (MQ=255)               cAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTccc                                                                                                                     >  1:35888/1‑140 (MQ=255)                               acaTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTa                                                                                                     <  1:671356/140‑1 (MQ=255)                                          tACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg                                                                                          >  1:340065/1‑140 (MQ=255)                                          tACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg                                                                                          >  2:726081/1‑140 (MQ=255)                                          tACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg                                                                                          >  2:127580/1‑140 (MQ=255)                                               tGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCaaaa                                                                                     <  2:487991/140‑1 (MQ=255)                                                  aCAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGtt                                                                                  <  2:35888/140‑1 (MQ=255)                                                  aCAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGtt                                                                                  <  2:55294/140‑1 (MQ=255)                                                    aGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGtttt                                                                                >  2:254855/1‑140 (MQ=255)                                                    aGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGtttt                                                                                <  1:127580/140‑1 (MQ=255)                                                    aGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGtttt                                                                                >  1:291953/1‑140 (MQ=255)                                                      cTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTtc                                                                              >  1:76823/1‑140 (MQ=255)                                                       ttGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTATGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTtct                                                                             <  1:707599/140‑1 (MQ=255)                                                         gTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa                                                                           >  1:773166/1‑140 (MQ=255)                                                         gTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa                                                                           >  2:97213/1‑140 (MQ=255)                                                         gTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa                                                                           >  1:584969/1‑140 (MQ=255)                                                         gTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa                                                                           >  1:48813/1‑140 (MQ=255)                                                         gTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCACACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa                                                                           <  2:706266/140‑1 (MQ=255)                                                          tCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCaa                                                                          <  1:254855/140‑1 (MQ=255)                                                           cGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAGTTTTACTAAACTATAGCAAAAGTTTTTCTCAAt                                                                         >  2:317848/1‑140 (MQ=255)                                                              cGTATTCATGGCGGTTCACTTGCATAGTCTTTCCGACGGGCATTTCCGGGCGTGCTAAATCAGCGTAAGCCGCGGGTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCt                                                                      >  2:78942/1‑140 (MQ=255)                                                               gTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTg                                                                     <  1:78129/140‑1 (MQ=255)                                                               gTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTg                                                                     <  1:349290/140‑1 (MQ=255)                                                                             tCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATgg                                                       >  2:221891/1‑140 (MQ=255)                                                                             tCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATgg                                                       >  1:624619/1‑140 (MQ=255)                                                                              cACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGga                                                      <  2:506132/140‑1 (MQ=255)                                                                                   ccACAATCTCTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATg                                                 <  2:232366/140‑1 (MQ=255)                                                                                               cGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATca                                     <  2:609886/140‑1 (MQ=255)                                                                                                  cgcgCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACAt                                  <  1:396427/140‑1 (MQ=255)                                                                                                    cgcATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTc                                >  2:491093/1‑140 (MQ=255)                                                                                                      cATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGt                              <  2:340065/140‑1 (MQ=255)                                                                                                            cAGACGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCg                        <  2:291953/140‑1 (MQ=255)                                                                                                               aCGTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCtg                     >  1:177258/1‑140 (MQ=255)                                                                                                                 gTGCAAAATCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCtgtg                   >  2:704743/1‑140 (MQ=255)                                                                                                                         tCAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGtt           >  1:471381/1‑140 (MQ=255)                                                                                                                          cAGCGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTa          <  1:726081/140‑1 (MQ=255)                                                                                                                             cGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAg       >  2:731179/1‑140 (MQ=255)                                                                                                                             cGTAAGCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAg       >  1:346866/1‑140 (MQ=255)                                                                                                                                  gCCACGGCTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa  <  2:773166/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCA  >  NC_000913/3221105‑3221373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑