Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,983	T→C	E253E (GAA→GAG)	uxaC ←	D‑glucoronate/D‑galacturonate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,983	0	T	C	100.0%	148.4 / NA	42	E253E (GAA→GAG)	uxaC	D‑glucoronate/D‑galacturonate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/24);  total (18/24)

TACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGT  >  NC_000913/3243855‑3244110                                                                                                                                 |                                                                                                                                tACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCttcgtt                                                                                                                      <  1:570543/140‑1 (MQ=255)    tgctgTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGc                                                                                                                   <  1:532353/140‑1 (MQ=255)      ctgTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCtt                                                                                                                 >  1:119783/1‑140 (MQ=255)      ctgTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCtt                                                                                                                 >  2:356592/1‑140 (MQ=255)      ctgTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCtt                                                                                                                 <  1:469908/140‑1 (MQ=255)           cccAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTAGCTTCAGCa                                                                                                            <  1:258134/140‑1 (MQ=255)              aGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAAc                                                                                                         >  2:804433/1‑140 (MQ=255)              aGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAAc                                                                                                         >  2:225208/1‑140 (MQ=255)               gCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACa                                                                                                        <  1:225208/140‑1 (MQ=255)                ccGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCttcgtt                                                                                                                      >  1:279626/1‑125 (MQ=255)                ccGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCttcgtt                                                                                                                      <  2:279626/125‑1 (MQ=255)                ccGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACa                                                                                                        >  2:499162/1‑139 (MQ=255)                ccGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACa                                                                                                        <  1:499162/139‑1 (MQ=255)                ccGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACAt                                                                                                       >  1:225321/1‑140 (MQ=255)                                    aCCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGt                                                                                   >  2:82346/1‑140 (MQ=255)                                     ccAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACAt                                                                                               <  2:734735/127‑1 (MQ=255)                                     ccAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACAt                                                                                               >  1:734735/1‑127 (MQ=255)                                      cAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCa                                                                                 <  1:578514/140‑1 (MQ=255)                                        aGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               <  1:520077/140‑1 (MQ=255)                                        aGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               <  1:493233/140‑1 (MQ=255)                                        aGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               <  2:369225/140‑1 (MQ=255)                                             cacCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               <  2:784876/135‑1 (MQ=255)                                             cacCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               >  1:784876/1‑135 (MQ=255)                                                  gTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               <  1:422278/130‑1 (MQ=255)                                                  gTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGa                                                                               >  2:422278/1‑130 (MQ=255)                                                   tACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGc                                                                    >  1:410985/1‑140 (MQ=255)                                                     ccGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCg                                                                  >  1:106143/1‑140 (MQ=255)                                                      cGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCGCCACATCCAGCGCGTGGTCAGACACTTTACAGCCGt                                                                 <  2:149366/140‑1 (MQ=255)                                                      cGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGt                                                                 >  1:493292/1‑140 (MQ=255)                                                           ttttGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAgcg                                                            <  1:82346/140‑1 (MQ=255)                                                              tGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAgcggcg                                                         <  2:225321/140‑1 (MQ=255)                                                                        cACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCa                                               <  1:356592/140‑1 (MQ=255)                                                                                     ctcAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAg                                  >  2:750082/1‑140 (MQ=255)                                                                                       cAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAggg                                <  2:253537/140‑1 (MQ=255)                                                                                       cAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACAGCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAggg                                <  2:119783/140‑1 (MQ=255)                                                                                         gggTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGt                                          <  1:400926/128‑1 (MQ=255)                                                                                         gggTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGt                                          >  2:400926/1‑128 (MQ=255)                                                                                              tCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAgg                                 >  2:626529/1‑132 (MQ=255)                                                                                              tCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAgg                                 <  1:626529/132‑1 (MQ=255)                                                                                              tCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTg                         <  1:306552/140‑1 (MQ=255)                                                                                              tCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTg                         <  1:174047/140‑1 (MQ=255)                                                                                                    gccagACGGCGCGCCAGAATGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTc                   >  1:700860/1‑140 (MQ=255)                                                                                                                     aaTGCTGTCCAGCTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGt  <  2:410985/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                TACTGCTGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGT  >  NC_000913/3243855‑3244110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑