Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,370,634	T→C	T135A (ACG→GCG)	nanE ←	putative N‑acetylmannosamine‑6‑phosphate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,370,634	0	T	C	100.0%	165.1 / NA	47	T135A (ACG→GCG)	nanE	putative N‑acetylmannosamine‑6‑phosphate epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

TCCGGCGTCGCTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAC  >  NC_000913/3370503‑3370766                                                                                                                                    |                                                                                                                                     tCCGGCGTCGCTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGt                                                                                                                              <  2:615729/140‑1 (MQ=255)    ggCGTCGCTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCgg                                                                                                                           >  2:206527/1‑140 (MQ=255)        tCGCTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCAt                                                                                                                       <  2:37464/140‑1 (MQ=255)         cGCTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATc                                                                                                                      >  1:448573/1‑140 (MQ=255)           cTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCAt                                                                                                                       <  2:640694/137‑1 (MQ=255)           cTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCAt                                                                                                                       >  1:640694/1‑137 (MQ=255)                       aCCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTg                                                                                                        <  1:299536/140‑1 (MQ=255)                                      ggCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATa                                                                                              <  2:627062/135‑1 (MQ=255)                                      ggCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATa                                                                                              >  1:627062/1‑135 (MQ=255)                                         tcttctGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCcag                                                                                      <  2:279476/140‑1 (MQ=255)                                          cttctGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCcagc                                                                                     <  2:448573/140‑1 (MQ=255)                                          cttctGGCGGTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCcagc                                                                                     <  1:118533/140‑1 (MQ=255)                                             ctGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCagcagc                                                                                  >  2:180564/1‑140 (MQ=255)                                                     tCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAAc                                                                          >  2:108296/1‑140 (MQ=255)                                                     tCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAAc                                                                          >  2:758900/1‑140 (MQ=255)                                                     tCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAAc                                                                          >  1:271351/1‑140 (MQ=255)                                                              gTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCgg                                                                 >  2:91968/1‑140 (MQ=255)                                                              gTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCgg                                                                 >  2:589262/1‑140 (MQ=255)                                                                   gCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGAcgcg                                                            >  2:310600/1‑140 (MQ=255)                                                                    ccAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgg                                                           >  1:630391/1‑140 (MQ=255)                                                                      aGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggc                                                         >  2:796049/1‑140 (MQ=255)                                                                        aaaGCGTAGTGCCAATAATTTCGGCTCCCAACTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggcgg                                                       >  1:518834/1‑140 (MQ=255)                                                                         aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggt                                                      <  1:281067/140‑1 (MQ=255)                                                                         aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggt                                                      >  2:130825/1‑140 (MQ=255)                                                                         aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggt                                                      >  2:529170/1‑140 (MQ=255)                                                                         aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggt                                                      <  1:536617/140‑1 (MQ=255)                                                                           gCGTAGTGCCAATAATTTCGGCTCCCAACTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                    <  2:518834/140‑1 (MQ=255)                                                                              tAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTg                                                 >  2:486730/1‑140 (MQ=255)                                                                                    cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCa                                           <  1:310600/140‑1 (MQ=255)                                                                                      ataatTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAt                                         >  2:115233/1‑140 (MQ=255)                                                                                       taatTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATg                                        <  1:340140/140‑1 (MQ=255)                                                                                        aatTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATgg                                       <  1:206527/140‑1 (MQ=255)                                                                                             cGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATa                                  <  2:339610/140‑1 (MQ=255)                                                                                                             gCATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCt                  >  2:432419/1‑140 (MQ=255)                                                                                                              cATGCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTg                 <  1:130825/140‑1 (MQ=255)                                                                                                                 gCCAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTgcgc              >  1:459443/1‑140 (MQ=255)                                                                                                                   cAGGCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCa            <  1:529170/140‑1 (MQ=255)                                                                                                                     ggCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGTGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgc          >  2:118058/1‑140 (MQ=255)                                                                                                                      gCCGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGGCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg         >  2:607797/1‑140 (MQ=255)                                                                                                                        cGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCa       <  1:796049/140‑1 (MQ=255)                                                                                                                        cGTCTTCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCa       <  2:630391/140‑1 (MQ=255)                                                                                                                             tCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGGGCATCAAc  <  1:486730/140‑1 (MQ=255)                                                                                                                             tCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc  <  1:432419/140‑1 (MQ=255)                                                                                                                             tCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc  <  1:108296/140‑1 (MQ=255)                                                                                                                             tCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc  <  1:725677/140‑1 (MQ=255)                                                                                                                             tCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc  <  1:198462/140‑1 (MQ=255)                                                                                                                             tCCGGCGCTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc  <  1:180564/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     TCCGGCGTCGCTCAACGTTTTCACCAGCGCCAGATCCGGCTCTTCTGGCGTTTCAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAC  >  NC_000913/3370503‑3370766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑