Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,112	G→A	A82T (GCG→ACG)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,112	0	G	A	100.0%	166.3 / NA	48	A82T (GCG→ACG)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/21);  total (27/21)

ATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCA  >  NC_000913/3374977‑3375247                                                                                                                                        |                                                                                                                                        atgTGCGGTGTCTGGTAAGGGATCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGt                                                                                                                                     >  2:573471/1‑140 (MQ=255)   gtgCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTGCTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTgg                                                                                                                                   >  2:601978/1‑140 (MQ=255)    tgCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTggg                                                                                                                                  >  1:360950/1‑140 (MQ=255)           tCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAACGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTAt                                                                                                                           <  1:600647/140‑1 (MQ=255)              ggTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGcc                                                                                                                        >  1:807979/1‑140 (MQ=255)              ggTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGcc                                                                                                                        >  1:437430/1‑140 (MQ=255)                  aGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCt                                                                                                                    <  2:437430/140‑1 (MQ=255)                   gggTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTa                                                                                                                   >  1:509730/1‑140 (MQ=255)                    ggTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTAc                                                                                                                  <  2:807979/140‑1 (MQ=255)                     gTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACa                                                                                                                 >  2:74444/1‑140 (MQ=255)                     gTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACa                                                                                                                 >  1:214681/1‑140 (MQ=255)                        cGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATg                                                                                                               <  1:466324/139‑1 (MQ=255)                        cGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATg                                                                                                               >  2:466324/1‑139 (MQ=255)                          gtggtgTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAg                                                                                                            <  1:573471/140‑1 (MQ=255)                                  cTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAgg                                                                                                    >  1:765330/1‑140 (MQ=255)                                       tCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCa                                                                                               >  2:332660/1‑140 (MQ=255)                                       tCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCa                                                                                               >  2:493430/1‑140 (MQ=255)                                       tCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCa                                                                                               >  2:136662/1‑140 (MQ=255)                                          gaccaAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATGCTGCGCATGCTGTCCAACCTCATTGCCGGATTGGGGCTGTTGCTTATGACGGTGGGCGGTTATACCGGCTACATGGAGCGCGTAGCGGctaatc                                                                                            >  2:320359/2‑135 (MQ=255)                                          caccaAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTc                                                                                            >  2:64471/1‑140 (MQ=255)                                          caccaAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTc                                                                                            >  1:439013/1‑140 (MQ=255)                                             caAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTcgcg                                                                                         <  1:537090/140‑1 (MQ=255)                                                 agcagcGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGAt                                                                                     >  1:101964/1‑140 (MQ=255)                                                      cGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGa                                                                                >  1:582515/1‑140 (MQ=255)                                                                   gtcgATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAgccg                                                                   <  1:183744/140‑1 (MQ=255)                                                                                        cTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgctc                                              >  2:386822/1‑140 (MQ=255)                                                                                         tGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgctcg                                             <  2:439013/140‑1 (MQ=255)                                                                                         tGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgctcg                                             <  2:450247/140‑1 (MQ=255)                                                                                         tGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgctcg                                             <  2:403896/140‑1 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgct                                               <  2:800929/136‑1 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgct                                               >  1:800929/1‑136 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           >  1:677544/1‑140 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           >  2:616414/1‑140 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           <  2:697399/140‑1 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           <  1:105646/140‑1 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           <  1:332660/140‑1 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           <  1:652353/140‑1 (MQ=255)                                                                                           cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGcc                                           <  1:493430/140‑1 (MQ=255)                                                                                                     ccAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgctcg                                             <  2:426210/128‑1 (MQ=255)                                                                                                     ccAACCGCATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATtcgctcg                                             >  1:426210/1‑128 (MQ=255)                                                                                                            cATTGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCgg                          >  1:672933/1‑140 (MQ=255)                                                                                                              ttGCCGGATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCa                        >  1:31414/1‑140 (MQ=255)                                                                                                                    gATTGGGGCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTAc                  >  1:320946/1‑140 (MQ=255)                                                                                                                        ggggCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCa              <  1:601978/140‑1 (MQ=255)                                                                                                                        ggggCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCa              <  2:201556/140‑1 (MQ=255)                                                                                                                         gggCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCAt             >  1:395583/1‑140 (MQ=255)                                                                                                                           gCTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCg           <  1:136662/140‑1 (MQ=255)                                                                                                                            cTGTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCgg          <  2:677544/140‑1 (MQ=255)                                                                                                                              gTCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGTGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGcc        >  2:791613/1‑140 (MQ=255)                                                                                                                               tCGATTATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCa       >  1:43126/1‑140 (MQ=255)                                                                                                                                    tATGACGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCa  <  1:320359/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCGCATAGGGGCCAGTCGCGCGATGGTGAGCTTGTTAAGCCGCCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCA  >  NC_000913/3374977‑3375247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑