Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	272,726	A→G	intergenic (+72/‑121)	eyeA → / → ykfC	CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	272,726	0	A	G	100.0%	182.0 / NA	51	intergenic (+72/‑121)	eyeA/ykfC	CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/29);  total (22/29)

TGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAA  >  NC_000913/272591‑272853                                                                                                                                        |                                                                                                                                tGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGcgcg                                                                                                                             >  1:507415/1‑140 (MQ=255)     ttGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCgctgg                                                                                                                         >  2:140002/1‑140 (MQ=255)        cTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCgctggctg                                                                                                                      >  1:245858/1‑140 (MQ=255)            cTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGTTGGCTGGCgg                                                                                                                  <  1:182942/140‑1 (MQ=255)            cTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCgg                                                                                                                  <  2:737659/140‑1 (MQ=255)             ttCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGa                                                                                                                 <  1:140002/140‑1 (MQ=255)               cTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGc                                                                                                               <  2:431771/140‑1 (MQ=255)               cTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGc                                                                                                               <  2:499882/140‑1 (MQ=255)                tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCt                                                                                                              >  2:376300/1‑140 (MQ=255)                 gCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCtt                                                                                                             <  2:630157/140‑1 (MQ=255)                  ccAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTc                                                                                                            >  1:740055/1‑140 (MQ=255)                       ttGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCgtag                                                                                                       >  1:399012/1‑140 (MQ=255)                            gcaagcaaGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTc                                                                                                  <  2:280545/140‑1 (MQ=255)                                       tAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa                                                                                       <  1:212023/140‑1 (MQ=255)                                       tAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa                                                                                       <  1:316066/140‑1 (MQ=255)                                         aaGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGaaa                                                                                     <  2:507415/140‑1 (MQ=255)                                               gCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGaaa                                                                                     >  1:404996/1‑134 (MQ=255)                                               gCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGaaa                                                                                     <  2:404996/134‑1 (MQ=255)                                               gCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAt                                                                               >  1:790163/1‑140 (MQ=255)                                                                cAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg                                                              <  1:393292/140‑1 (MQ=255)                                                                cAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg                                                              <  2:589590/140‑1 (MQ=255)                                                                cAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg                                                              <  1:209684/140‑1 (MQ=255)                                                                cAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg                                                              <  2:177388/140‑1 (MQ=255)                                                                cAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg                                                              >  2:217547/1‑140 (MQ=255)                                                                   tATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAg                                                           >  2:658828/1‑140 (MQ=255)                                                                      gCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGttt                                                        >  1:105293/1‑140 (MQ=255)                                                                        cTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGa                                                      <  2:577954/140‑1 (MQ=255)                                                                        cTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGa                                                      >  1:800681/1‑140 (MQ=255)                                                                        cTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGa                                                      >  2:372758/1‑140 (MQ=255)                                                                                 ggcaggTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTg                                             <  2:800681/140‑1 (MQ=255)                                                                                    aggTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGa                                          <  2:245858/140‑1 (MQ=255)                                                                                    aggTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGa                                          <  2:668808/140‑1 (MQ=255)                                                                                     ggTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGAc                                         <  2:105293/140‑1 (MQ=255)                                                                                      gTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACg                                        <  2:418529/140‑1 (MQ=255)                                                                                       tAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGt                                       >  2:647841/1‑140 (MQ=255)                                                                                         aaCCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATTGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACg                                        <  1:606806/137‑1 (MQ=255)                                                                                         aaCCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATTGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACg                                        >  2:606806/1‑137 (MQ=255)                                                                                              gggAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTaa                                >  2:240044/1‑140 (MQ=255)                                                                                              gggAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTaa                                <  1:217547/140‑1 (MQ=255)                                                                                               ggAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAAc                               <  1:372758/140‑1 (MQ=255)                                                                                                   ggTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGAc                           <  1:658828/140‑1 (MQ=255)                                                                                                   ggTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGAc                           <  1:649947/140‑1 (MQ=255)                                                                                                      cAGCGACGGATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTa                        <  2:399012/140‑1 (MQ=255)                                                                                                             ggATGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGt                 >  1:102035/1‑140 (MQ=255)                                                                                                               aTGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGa               >  2:371101/1‑140 (MQ=255)                                                                                                                tGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGaa              <  1:376300/140‑1 (MQ=255)                                                                                                                tGTTAAGGGGGCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGaa              <  1:400359/140‑1 (MQ=255)                                                                                                                         ggCATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGa     >  1:501546/1‑140 (MQ=255)                                                                                                                           cATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATa   >  1:403215/1‑140 (MQ=255)                                                                                                                           cATGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATa   >  2:411441/1‑140 (MQ=255)                                                                                                                            aTGGCTCCGATGGCGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATaa  >  1:208061/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                TGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAA  >  NC_000913/272591‑272853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑