Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,389,224 T→C T38T (ACA→ACG)  aaeX ← DUF1656 domain‑containing protein AaeX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,389,2240TC100.0% 194.5 / NA 54T38T (ACA→ACG) aaeXDUF1656 domain‑containing protein AaeX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/30);  total (24/30)

ATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTGGTTGTAAA  >  NC_000913/3389089‑3389355
                                                                                                                                       |                                                                                                                                   
aTGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTg                                                                                                                                 >  1:225726/1‑140 (MQ=255)
aTGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTg                                                                                                                                 >  1:432685/1‑140 (MQ=255)
   gCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGca                                                                                                                              <  2:225726/140‑1 (MQ=255)
    ccGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGcac                                                                                                                             <  1:167584/140‑1 (MQ=255)
        aCGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGt                                                                                                                         >  1:757687/1‑140 (MQ=255)
         cGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTa                                                                                                                        <  1:591174/140‑1 (MQ=255)
                 ttttCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAGAGTCGTAGATACCCGTTGGCACAAGTACCCGgcgc                                                                                                                >  2:558523/1‑140 (MQ=255)
                    tCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCAcc                                                                                                             <  2:732240/140‑1 (MQ=255)
                        attaGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCAccagcc                                                                                                         <  1:333806/140‑1 (MQ=255)
                          taGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCAccagccag                                                                                                       <  1:152215/140‑1 (MQ=255)
                          taGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCAccagccag                                                                                                       <  1:201089/140‑1 (MQ=255)
                                    aCTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCa                                                                                             <  1:497738/140‑1 (MQ=255)
                                           ccTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATaa                                                                                      >  2:288008/1‑140 (MQ=255)
                                           ccTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATaa                                                                                      >  1:733917/1‑140 (MQ=255)
                                             tCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATaaaa                                                                                    <  2:757687/140‑1 (MQ=255)
                                                aaCGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCa                                                                                 >  2:668739/1‑140 (MQ=255)
                                                aaCGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCa                                                                                 >  1:87845/1‑140 (MQ=255)
                                                      cAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCaa                                                                           >  1:422621/1‑140 (MQ=255)
                                                             gATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAatat                                                                    <  1:82349/140‑1 (MQ=255)
                                                               tatCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACGAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCg                                                                  <  2:432685/140‑1 (MQ=255)
                                                               tatCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCg                                                                  <  2:581455/140‑1 (MQ=255)
                                                               tatCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCg                                                                  <  1:563764/140‑1 (MQ=255)
                                                               tatCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCg                                                                  <  1:227585/140‑1 (MQ=255)
                                                                     aTAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGa                                                            >  1:30163/1‑140 (MQ=255)
                                                                         aaCAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGa                                                        >  2:425508/1‑140 (MQ=255)
                                                                          aCAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGAc                                                       <  1:425508/140‑1 (MQ=255)
                                                                          aCAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGAc                                                       <  2:422621/140‑1 (MQ=255)
                                                                            aaGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAg                                                     <  1:408231/140‑1 (MQ=255)
                                                                             agcagcAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGc                                                    >  2:225216/1‑140 (MQ=255)
                                                                                  caATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCaaa                                               <  1:225216/140‑1 (MQ=255)
                                                                                          cgcgGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGa                                       >  1:76943/1‑140 (MQ=255)
                                                                                          cgcgGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGa                                       >  1:165126/1‑140 (MQ=255)
                                                                                            cgGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGAt                                      >  1:298195/1‑139 (MQ=255)
                                                                                            cgGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGAt                                      <  2:298195/139‑1 (MQ=255)
                                                                                            cgGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATa                                     >  1:109841/1‑140 (MQ=255)
                                                                                            cgGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATa                                     >  2:334292/1‑140 (MQ=255)
                                                                                                    aacaacGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGaaa                             <  1:452631/140‑1 (MQ=255)
                                                                                                    aacaacGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGaaa                             <  2:465559/140‑1 (MQ=255)
                                                                                                      caacGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACa                           <  1:51672/140‑1 (MQ=255)
                                                                                                         cGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGa                                       >  1:429790/1‑125 (MQ=255)
                                                                                                         cGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGa                                       <  2:429790/125‑1 (MQ=255)
                                                                                                         cGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGAc                        <  2:165126/140‑1 (MQ=255)
                                                                                                         cGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGAc                        >  2:736737/1‑140 (MQ=255)
                                                                                                         cGCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGAc                        >  2:501679/1‑140 (MQ=255)
                                                                                                          gCCGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACt                       <  1:668739/140‑1 (MQ=255)
                                                                                                           ccGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTc                      >  2:474571/1‑140 (MQ=255)
                                                                                                           ccGGATGCCAGACAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTc                      >  1:132483/1‑140 (MQ=255)
                                                                                                                       cAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTg          <  1:349526/140‑1 (MQ=255)
                                                                                                                       cAAAGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTg          <  2:30163/140‑1 (MQ=255)
                                                                                                                        aaaGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTg          >  2:189090/1‑139 (MQ=255)
                                                                                                                        aaaGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTg          <  1:189090/139‑1 (MQ=255)
                                                                                                                         aaGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTGGt        <  1:432984/140‑1 (MQ=255)
                                                                                                                          aGTCGTAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACTGGAAACAGACTCATGTTGACCTTGGtg       >  2:566617/1‑139 (MQ=255)
                                                                                                                               tAGATACCCGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTGGTTGTaaa  <  2:166995/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
ATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACGATAACGGGAAACAGACTCATGTTGACCTTGGTTGTAAA  >  NC_000913/3389089‑3389355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑