Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,416,925	A→G	K631K (AAA→AAG)	acrF →	multidrug efflux pump RND permease AcrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,416,925	0	A	G	100.0%	173.8 / NA	49	K631K (AAA→AAG)	acrF	multidrug efflux pump RND permease AcrF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/28);  total (21/28)

ACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGG  >  NC_000913/3416788‑3417058                                                                                                                                          |                                                                                                                                      aCGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGcc                                                                                                                                     <  2:520401/140‑1 (MQ=255)  cGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCg                                                                                                                                    >  2:197013/1‑140 (MQ=255)     aaaaaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTgg                                                                                                                                 >  2:804270/1‑140 (MQ=255)      aaaaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTggg                                                                                                                                <  2:366502/140‑1 (MQ=255)        aaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGaa                                                                                                                              <  2:220413/140‑1 (MQ=255)        aaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGaa                                                                                                                              <  1:693297/140‑1 (MQ=255)        aaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGaa                                                                                                                              <  2:28467/140‑1 (MQ=255)            gtTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGTACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGc                                                                                                                          <  2:5231/140‑1 (MQ=255)                            gATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGaaaa                                                                                                          <  2:251592/140‑1 (MQ=255)                               tACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAg                                                                                                       >  2:790323/1‑140 (MQ=255)                               tACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAg                                                                                                       >  2:404729/1‑140 (MQ=255)                                aCTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGCCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGt                                                                                                      >  1:554414/1‑140 (MQ=255)                                    tCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCAGGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGCACTGGTGACGAAAACAGTGCgg                                                                                                  >  2:200103/1‑140 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCgg                                                                                                  >  2:38474/1‑137 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCgg                                                                                                  <  1:38474/137‑1 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGa                                                                                                 <  1:744764/138‑1 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGa                                                                                                 >  2:744764/1‑138 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAg                                                                                               <  2:729647/140‑1 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAg                                                                                               <  1:790323/140‑1 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAg                                                                                               <  1:804270/140‑1 (MQ=255)                                       gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAg                                                                                               >  2:167296/1‑140 (MQ=255)                                           aaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGt                                                                                           >  2:474191/1‑140 (MQ=255)                                              gagaAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAAt                                                                                        <  1:267003/140‑1 (MQ=255)                                                  aaGCGAACGTTGAAA‑‑GTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCg                                                                                  <  1:620498/140‑1 (MQ=255)                                                   aGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATc                                                                                   >  1:234408/1‑140 (MQ=255)                                                       aaCGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGc                                                                               <  1:193208/140‑1 (MQ=255)                                                       aaCGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGc                                                                               <  1:197013/140‑1 (MQ=255)                                                                    tCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTTGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTg                                                                  >  1:608534/1‑140 (MQ=255)                                                                    tCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTg                                                                  >  2:121645/1‑140 (MQ=255)                                                                      tttACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGc                                                                               <  1:661152/125‑1 (MQ=255)                                                                      tttACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGc                                                                               >  2:661152/1‑125 (MQ=255)                                                                       ttACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGc                                                               <  2:385251/140‑1 (MQ=255)                                                                        tACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCa                                                              <  1:167296/140‑1 (MQ=255)                                                                            gTTAACGGCTTTAGCTTCAGCGGCCA‑GCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATc                                                         >  1:457327/1‑140 (MQ=255)                                                                                 cGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcgcg                                                     <  1:321990/140‑1 (MQ=255)                                                                                 cGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcgcg                                                     <  2:489442/140‑1 (MQ=255)                                                                                       tAGCTTCAGCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGtt                                               >  1:760278/1‑140 (MQ=255)                                                                                               gCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcatt                                       <  1:495550/140‑1 (MQ=255)                                                                                               gCGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcatt                                       <  1:404729/140‑1 (MQ=255)                                                                                                cGGCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattc                                      >  2:90722/1‑140 (MQ=255)                                                                                                 ggCCA‑GCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattcca                                    <  2:457327/140‑1 (MQ=255)                                                                                                  gCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattcca                                    >  1:320586/1‑140 (MQ=255)                                                                                                  gCCAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattcca                                    <  1:662752/140‑1 (MQ=255)                                                                                                   ccAGGCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattccat                                   >  1:253208/1‑140 (MQ=255)                                                                                                      ggCACAAAACGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCa                                <  2:625254/140‑1 (MQ=255)                                                                                                             aaCGCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATgcc                         <  2:234408/140‑1 (MQ=255)                                                                                                                gCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATgccagc                      <  2:792233/140‑1 (MQ=255)                                                                                                                gCCGGCATGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATgccagc                      <  1:90722/140‑1 (MQ=255)                                                                                                                  cggcaTGGCCTTCGTCAGTCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATgccagcca                    >  1:659471/5‑140 (MQ=255)                                                                                                                                    tCTGAAGCCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACgg  >  2:554771/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      ACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGG  >  NC_000913/3416788‑3417058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑