Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,445,283	T→C	A105A (GCA→GCG)	rplR ←	50S ribosomal subunit protein L18

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,445,283	0	T	C	100.0%	160.1 / NA	45	A105A (GCA→GCG)	rplR	50S ribosomal subunit protein L18
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/19);  total (26/19)

TTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGTGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAGTTGTT  >  NC_000913/3445153‑3445419                                                                                                                                   |                                                                                                                                         ttAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGaca                                                                                                                                 <  1:698435/140‑1 (MQ=255) ttAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGaca                                                                                                                                 <  1:637609/140‑1 (MQ=255)       gTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCa                                                                                                                           <  2:288966/140‑1 (MQ=255)         tttAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATg                                                                                                                         >  1:582420/1‑140 (MQ=255)                  gcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGaa                                                                                                                >  2:545290/1‑140 (MQ=255)                  gcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGaa                                                                                                                >  1:682385/1‑140 (MQ=255)                      tttACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCg                                                                                                            <  2:582420/140‑1 (MQ=255)                         aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         <  1:277058/140‑1 (MQ=255)                         aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         >  2:252471/1‑140 (MQ=255)                         aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         <  2:146142/140‑1 (MQ=255)                            gcgATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACgg                                                                                                      <  2:265114/140‑1 (MQ=255)                               aTCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCa                                                                                                   <  2:682385/140‑1 (MQ=255)                               aTCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCa                                                                                                   <  2:358834/140‑1 (MQ=255)                                tCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCaa                                                                                                  >  2:428704/1‑140 (MQ=255)                                  aGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCa                                                                                                   <  1:731052/137‑1 (MQ=255)                                  aGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCa                                                                                                   >  2:731052/1‑137 (MQ=255)                                    cTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGa                                                                                              <  2:418100/140‑1 (MQ=255)                                            gCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCtt                                                                                      >  1:663864/1‑140 (MQ=255)                                                  cGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGc                                                                                >  2:358524/1‑140 (MQ=255)                                                   gCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGcc                                                                               <  2:689248/140‑1 (MQ=255)                                                    ccAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCt                                                                              <  1:188178/140‑1 (MQ=255)                                                             ttttCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGcc                                                                               >  1:230359/1‑130 (MQ=255)                                                             ttttCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGcc                                                                               <  2:230359/130‑1 (MQ=255)                                                                                       ctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  1:355301/1‑140 (MQ=255)                                                                                         aCCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAg                                         >  2:200233/1‑140 (MQ=255)                                                                                          ccTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGc                                        >  2:408658/1‑140 (MQ=255)                                                                                          ccTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGc                                        >  1:316793/1‑140 (MQ=255)                                                                                             tAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGc                                     >  2:275121/1‑140 (MQ=255)                                                                                               gAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAg                                   >  1:300824/1‑140 (MQ=255)                                                                                               gAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAg                                   >  2:522938/1‑140 (MQ=255)                                                                                                  cTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGcc                                 <  2:698519/139‑1 (MQ=255)                                                                                                  cTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGcc                                 >  1:698519/1‑139 (MQ=255)                                                                                                  cTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcg                                >  1:638626/1‑140 (MQ=255)                                                                                                  cTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcg                                >  1:320638/1‑140 (MQ=255)                                                                                                    gAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcgcg                              >  1:251684/1‑140 (MQ=255)                                                                                                    gAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcgcg                              >  2:422567/1‑140 (MQ=255)                                                                                                    gAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcgcg                              <  1:408658/140‑1 (MQ=255)                                                                                                            gCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGtt                      >  1:163719/1‑140 (MQ=255)                                                                                                              ttCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTAc                    <  1:331606/140‑1 (MQ=255)                                                                                                                  cGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGgt                >  1:17026/1‑140 (MQ=255)                                                                                                                   gggCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGgtg               <  2:440704/140‑1 (MQ=255)                                                                                                                       agcaTCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACt           >  2:463032/1‑140 (MQ=255)                                                                                                                         caTCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTc         >  1:218885/1‑140 (MQ=255)                                                                                                                         caTCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTc         >  2:755244/1‑140 (MQ=255)                                                                                                                                cAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAgttgtt  <  2:218885/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGTGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAGTTGTT  >  NC_000913/3445153‑3445419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑