Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,627,725	T→C	N605S (AAC→AGC)	rbbA ←	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,627,725	0	T	C	100.0%	175.7 / NA	49	N605S (AAC→AGC)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

CGCGCGATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGTTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGAA  >  NC_000913/3627593‑3627859                                                                                                                                     |                                                                                                                                       cgcgcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGa                                                                                                                                     >  2:482003/1‑136 (MQ=255) cgcgcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGa                                                                                                                                     <  1:482003/136‑1 (MQ=255)   cgcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTcc                                                                                                                               >  2:282095/1‑140 (MQ=255)   cgcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTcc                                                                                                                               >  1:375749/1‑140 (MQ=255)   cgcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTcc                                                                                                                               >  1:414912/1‑140 (MQ=255)   cgcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTcc                                                                                                                               >  2:696016/1‑140 (MQ=255)    gcgATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCa                                                                                                                              <  1:432446/140‑1 (MQ=255)      gATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGATGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACg                                                                                                                            <  1:681751/140‑1 (MQ=255)       atatCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGc                                                                                                                           <  1:755629/140‑1 (MQ=255)       atatCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCCTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGc                                                                                                                           <  2:712077/140‑1 (MQ=255)         atCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCt                                                                                                                         <  1:277888/140‑1 (MQ=255)            gcgcCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGc                                                                                                                      <  2:456307/140‑1 (MQ=255)             cgcCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGa                                                                                                                                     >  2:774079/1‑124 (MQ=255)             cgcCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGa                                                                                                                                     <  1:774079/124‑1 (MQ=255)                  gAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTc                                                                                                                                <  2:580508/124‑1 (MQ=255)                  gAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTc                                                                                                                                >  1:580508/1‑124 (MQ=255)                             ggATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTcg                                                                                                     <  1:619745/140‑1 (MQ=255)                             ggATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTcg                                                                                                     <  2:112828/140‑1 (MQ=255)                              gATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTcgc                                                                                                    >  1:53692/1‑140 (MQ=255)                                     aTCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAg                                                                                             <  1:369562/140‑1 (MQ=255)                                         ccACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCAcc                                                                                         >  1:810863/1‑140 (MQ=255)                                                    tCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAg                                                                              >  2:747713/1‑140 (MQ=255)                                                     cGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAgg                                                                             <  2:414912/140‑1 (MQ=255)                                                        ccGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGttt                                                                          <  1:282095/140‑1 (MQ=255)                                                            acgcaTCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCca                                                                      <  1:7288/140‑1 (MQ=255)                                                                aTCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATc                                                                  >  2:596735/1‑140 (MQ=255)                                                                            aGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGcc                                                      >  1:648044/1‑140 (MQ=255)                                                                                 gTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAAc                                                 <  1:747713/140‑1 (MQ=255)                                                                                 gTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAAc                                                 >  1:805981/1‑140 (MQ=255)                                                                                    aTAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCa                                              <  2:648044/140‑1 (MQ=255)                                                                                       aCTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTa                                                   <  1:483002/132‑1 (MQ=255)                                                                                       aCTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTa                                                   >  2:483002/1‑132 (MQ=255)                                                                                        cTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTAt                                          <  1:673954/140‑1 (MQ=255)                                                                                            tGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGc                                      <  1:696016/140‑1 (MQ=255)                                                                                                cggcggCTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCa                                  >  1:405999/1‑140 (MQ=255)                                                                                                      cTGTTCGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCa                            <  2:810863/140‑1 (MQ=255)                                                                                                           cGATAAAGTAACGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGtt                       <  2:677740/140‑1 (MQ=255)                                                                                                                     aCGGGAACCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAgcg             >  1:368311/1‑140 (MQ=255)                                                                                                                           aCCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCg       >  2:585179/1‑140 (MQ=255)                                                                                                                           aCCGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCg       <  1:387826/140‑1 (MQ=255)                                                                                                                            ccGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGt      >  1:136222/1‑140 (MQ=255)                                                                                                                             cGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTc     >  2:535904/1‑140 (MQ=255)                                                                                                                             cGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTc     <  1:157518/140‑1 (MQ=255)                                                                                                                             cGGAGAGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTc     >  2:795969/1‑140 (MQ=255)                                                                                                                               gagaGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGa   <  2:375749/140‑1 (MQ=255)                                                                                                                               gagaGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGa   <  1:565325/140‑1 (MQ=255)                                                                                                                               gagaGGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGa   <  2:805981/140‑1 (MQ=255)                                                                                                                                agagGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGaa  <  1:790910/140‑1 (MQ=255)                                                                                                                                agagGCTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGaa  >  2:790910/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       CGCGCGATATCGCGCCCGAAATTGGGCGGGATCTCAATCGCCACCGTGATATCGCCCGCACGCATCCGACGATCAAGCTCGTCATAACTGGTGAGCGGCGGCTGTTCGATAAAGTAACGGGAACCGGAGAGGTTGAGTGTCCACGCCTGGCTACTGACGGTCTGGTCGCGGTCGAGCACCGCAAAGCGCAGGTTTTCCACATCCATACTGATGCCGTAACCCATTATCAGCATCAGGATCACCGTTCCCATCAGCGCCAGCGTCGAA  >  NC_000913/3627593‑3627859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑