Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,077	G→A	T210I (ACC→ATC)	dppA ←	dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,077	0	G	A	100.0%	123.5 / NA	36	T210I (ACC→ATC)	dppA	dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/14);  total (22/14)

ACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAA  >  NC_000913/3706947‑3707212                                                                                                                                   |                                                                                                                                        aCGCACGGAATCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTg                                                                                                                                <  1:555296/140‑1 (MQ=255)                  gggTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGtttt                                                                                                               <  2:355518/140‑1 (MQ=255)                   ggTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACAGACCGATTCCGATTGGGTTGAGGTCCAGttttt                                                                                                              >  2:638782/1‑140 (MQ=255)                            gaAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTAc                                                                                                     <  2:105384/140‑1 (MQ=255)                            gaAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTAc                                                                                                     >  2:710924/1‑140 (MQ=255)                             aaaaCCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTAcc                                                                                                    >  2:737849/1‑140 (MQ=255)                                               tGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATc                                                                                  >  2:709640/1‑140 (MQ=255)                                                gCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCa                                                                                 <  1:737849/140‑1 (MQ=255)                                                       ggTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATAtt                                                                          >  1:369094/1‑140 (MQ=255)                                                       ggTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATAtt                                                                          >  2:495340/1‑140 (MQ=255)                                                       ggTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATAtt                                                                          >  2:728836/1‑140 (MQ=255)                                                         tGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCt                                                                        <  2:369094/140‑1 (MQ=255)                                                           ccccAGTAGCCATCAAACGCTTTGTAGCGGGTACGGGAATCTTTTTGGTACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGGGGTCCAGTTTTTCCGGTGTAACGGCTTTCATCATCGCATCAGCATATTCttt                                                                      >  2:187386/1‑140 (MQ=255)                                                                      aTCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATag                                                           >  2:614717/1‑140 (MQ=255)                                                                       tCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATaga                                                          >  1:767980/1‑140 (MQ=255)                                                                        cAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCTGTGTACCGGCTTTCATCATCGCATCAGCATATTCttt                                                                      >  1:724365/1‑127 (MQ=255)                                                                        cAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCTGTGTACCGGCTTTCATCATCGCATCAGCATATTCttt                                                                      <  2:724365/127‑1 (MQ=255)                                                                               tttGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGt                                                  <  2:228647/140‑1 (MQ=255)                                                                                 tGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGa                                                     <  1:267457/135‑1 (MQ=255)                                                                                 tGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGa                                                     >  2:267457/1‑135 (MQ=255)                                                                                         aTACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAg                                        >  1:157737/1‑140 (MQ=255)                                                                                         aTACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAg                                        >  1:466351/1‑140 (MQ=255)                                                                                         aTACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAg                                        >  1:243333/1‑140 (MQ=255)                                                                                           aCGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCAGCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGt                                      >  2:129517/1‑140 (MQ=255)                                                                                                     ttttGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGaa                            <  2:351046/140‑1 (MQ=255)                                                                                                       ttGATACTGCTGTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACg                          >  2:683646/1‑140 (MQ=255)                                                                                                                ctgTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCg                 >  1:690028/1‑140 (MQ=255)                                                                                                                  gTAACTGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCggg               <  1:495340/140‑1 (MQ=255)                                                                                                                       tGGAACGGACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCACTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGt          <  2:723369/140‑1 (MQ=255)                                                                                                                             ggACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGcac    >  1:236684/1‑140 (MQ=255)                                                                                                                             ggACCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGcac    <  2:466351/140‑1 (MQ=255)                                                                                                                               aCCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAg           <  2:574588/131‑1 (MQ=255)                                                                                                                               aCCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAg           >  1:574588/1‑131 (MQ=255)                                                                                                                               aCCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGt          <  1:762833/132‑1 (MQ=255)                                                                                                                               aCCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGt          >  2:762833/1‑132 (MQ=255)                                                                                                                               aCCGATTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACaa  >  2:452672/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        ACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAA  >  NC_000913/3706947‑3707212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑