Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,848,200	A→C	L142R (CTG→CGG)	uhpC ←	inner membrane protein sensing glucose‑6‑phosphate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,848,200	0	A	C	100.0%	145.6 / NA	41	L142R (CTG→CGG)	uhpC	inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/17);  total (24/17)

TCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATA  >  NC_000913/3848063‑3848334                                                                                                                                          |                                                                                                                                       tcatcCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGa                                                                                                                                      >  2:207004/1‑140 (MQ=255)       cGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGca                                                                                                                                <  2:593995/140‑1 (MQ=255)          cACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGcacac                                                                                                                             >  1:341058/1‑140 (MQ=255)                     tAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACC‑GGCCGTTAACCGACGCGCACACACCGGTGAAccc                                                                                                                 <  1:728248/140‑1 (MQ=255)                             cgcagcCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTg                                                                                                                      >  2:655555/1‑128 (MQ=255)                             cgcagcCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTg                                                                                                                      <  1:655555/128‑1 (MQ=255)                                     cTGCCATCACCATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAg                                                                                                  <  1:554754/140‑1 (MQ=255)                                       gCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGc                                                                                                <  2:593721/140‑1 (MQ=255)                                            cacaATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCa                                                                                           >  2:23077/1‑140 (MQ=255)                                                 tGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCAcc                                                                                      <  2:163457/140‑1 (MQ=255)                                                      aTGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCagag                                                                                 >  2:743922/1‑140 (MQ=255)                                                         aGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCAc                                                                              >  2:15959/1‑140 (MQ=255)                                                             cgccgcCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACGCCAGCCCTGGAAAGAGGCGTTCAGCACCCAGAGCGCGGCa                                                                          >  2:717317/1‑140 (MQ=255)                                                             cgccgcCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCa                                                                          >  2:676762/1‑140 (MQ=255)                                                               ccgccgACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCaaa                                                                        <  2:66780/140‑1 (MQ=255)                                                                                cGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCg                                                       >  2:286696/1‑140 (MQ=255)                                                                                  tgtTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgag                                                     <  1:717317/140‑1 (MQ=255)                                                                                  tgtTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgag                                                     >  1:754493/1‑140 (MQ=255)                                                                                            tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACa                                           >  1:162059/1‑140 (MQ=255)                                                                                            tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACACGCACACACCGGTGAACCCCAGCACTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACa                                           >  2:338051/1‑140 (MQ=255)                                                                                              cccACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGa                                         >  2:473570/1‑140 (MQ=255)                                                                                               ccaccaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGaa                                        <  1:153657/140‑1 (MQ=255)                                                                                                 accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATg                                      >  1:776199/1‑140 (MQ=255)                                                                                                 accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATg                                      >  1:300524/1‑140 (MQ=255)                                                                                                 accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATg                                      >  1:517016/1‑140 (MQ=255)                                                                                                   caACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACa                                           >  2:304776/1‑133 (MQ=255)                                                                                                   caACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACa                                           <  1:304776/133‑1 (MQ=255)                                                                                                      ccgccgCGCTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGAt                                 >  2:212360/1‑140 (MQ=255)                                                                                                            cgcTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGcc                           >  2:431086/1‑140 (MQ=255)                                                                                                             gcTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGccc                          >  2:511757/1‑140 (MQ=255)                                                                                                             gcTCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGccc                          >  2:60828/1‑140 (MQ=255)                                                                                                               tCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGt                        <  1:60828/140‑1 (MQ=255)                                                                                                               tCGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGt                        <  1:511757/140‑1 (MQ=255)                                                                                                                cGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTg                       <  2:300524/140‑1 (MQ=255)                                                                                                                cGGTACGTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTg                       <  2:517016/140‑1 (MQ=255)                                                                                                                      gTGAATACCAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATa                 <  1:212360/140‑1 (MQ=255)                                                                                                                            aCCAGGCCGTTAACCGACACGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCt           <  1:338051/140‑1 (MQ=255)                                                                                                                             ccAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTa          >  2:27249/1‑140 (MQ=255)                                                                                                                              cAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTAt         <  1:676762/140‑1 (MQ=255)                                                                                                                              cAGGCCGTTAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTAt         <  2:273704/140‑1 (MQ=255)                                                                                                                                     ttAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATa  >  1:238767/1‑140 (MQ=255)                                                                                                                                     ttAACCGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATa  >  2:470611/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATA  >  NC_000913/3848063‑3848334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑