Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,414	A→G	G14G (GGT→GGC)	mioC ←	flavoprotein MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,414	0	A	G	100.0%	196.4 / NA	56	G14G (GGT→GGC)	mioC	flavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/32);  total (24/32)

GCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGATCAGGG  >  NC_000913/3926280‑3926550                                                                                                                                       |                                                                                                                                         gCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGGAAAATCCGCCTCTTCCAACTTTTCAGCCAGGTGTTCTGCTACATATTCGGcgccgct                                                                                                                                     >  2:276210/1‑139 (MQ=255)      gtgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAggg                                                                                                                                >  2:270063/1‑140 (MQ=255)       tgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAgggg                                                                                                                               >  2:79086/1‑139 (MQ=255)       tgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAgggg                                                                                                                               >  2:779504/1‑139 (MQ=255)             gAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGcc                                                                                                                         <  1:664310/140‑1 (MQ=255)                   aTACCCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGCCCGTGCAGCGTTTCGGTGGTAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGAt                                                                                                                   <  1:370938/140‑1 (MQ=255)                   aTAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGAt                                                                                                                   <  1:453172/140‑1 (MQ=255)                   aTAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGAt                                                                                                                   >  2:370203/1‑140 (MQ=255)                    tAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATa                                                                                                                  <  1:242064/140‑1 (MQ=255)                           ccagATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGa                                                                                                           >  2:18919/1‑140 (MQ=255)                             agATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGata                                                                                                         >  1:783213/1‑140 (MQ=255)                              gATCCCTGAGGGAGGTAAATCTTCAAACAGCGGCCGGTGCAGCGTTTGGGGGGAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGatat                                                                                                        <  2:613022/140‑1 (MQ=255)                                  ccTGAGGCAGGTAAATCTTCTAACAGCGTACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGc                                                                                                    >  1:340105/1‑140 (MQ=255)                                    tGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCa                                                                                                  >  1:256983/1‑140 (MQ=255)                                     gAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGatat                                                                                                        >  2:677597/1‑133 (MQ=255)                                     gAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGatat                                                                                                        <  1:677597/133‑1 (MQ=255)                                     gAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCAt                                                                                                 >  2:505361/1‑140 (MQ=255)                                           ggTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGC‑‑CCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGcc                                                                                         >  1:422159/1‑140 (MQ=255)                                            gTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                                                                          <  1:545120/140‑1 (MQ=255)                                            gTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                                                                          <  1:157417/140‑1 (MQ=255)                                             tAAATCTTCTAACAGCGCACCGTGCAGCGTTTCGGGGGTAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGcc                                                                                         <  2:328848/140‑1 (MQ=255)                                                aTCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCAcc                                                                                      <  1:759366/140‑1 (MQ=255)                                                      tAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTttatt                                                                                >  2:681722/1‑140 (MQ=255)                                                      tAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTttatt                                                                                <  1:227765/140‑1 (MQ=255)                                                           gCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAagag                                                                           >  2:460505/1‑140 (MQ=255)                                                              gcccGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTgg                                                                        <  1:640504/138‑1 (MQ=255)                                                               ccggTGCGGCGTTTGGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGc                                                                       <  2:17682/137‑1 (MQ=255)                                                               cccGTGCAGCTTTTGGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGc                                                                       <  2:307530/139‑1 (MQ=255)                                                               cccGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGc                                                                       <  1:469102/139‑1 (MQ=255)                                                                  gTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTCCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTa                                                                    >  2:807911/1‑140 (MQ=255)                                                                     gaggttttGGGTGGAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTg                                                                 <  2:116794/135‑1 (MQ=255)                                                                         ttttGGGTGGAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACg                                                             <  2:783213/139‑1 (MQ=255)                                                                         gTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACg                                                             <  1:505361/140‑1 (MQ=255)                                                                          tttCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGc                                                            >  1:295568/1‑140 (MQ=255)                                                                                tggtAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGC‑‑CCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAAcg                                                    <  2:422159/140‑1 (MQ=255)                                                                                     aaccccGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGt                                                 <  1:13349/137‑1 (MQ=255)                                                                                     aaaCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGt                                                 <  2:295568/140‑1 (MQ=255)                                                                                          cGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGa                                            >  2:137968/1‑140 (MQ=255)                                                                                               ctTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTAc                                       <  1:624789/140‑1 (MQ=255)                                                                                                 tCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCt                                     >  1:454885/1‑140 (MQ=255)                                                                                                       ttttCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGaa                               <  2:340105/140‑1 (MQ=255)                                                                                                       ttttCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGaa                               <  2:454885/140‑1 (MQ=255)                                                                                                          tCAGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAg                            >  1:571397/1‑140 (MQ=255)                                                                                                            aGCCAGGTGTTCTGCTACATATTCGGCGCCGCTGAGGGTGCTGCTGTTGATAAGAGTGATATCTGCCATAAACCGCCCCCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTa                          <  1:276210/140‑1 (MQ=255)                                                                                                            aGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTa                          <  2:15873/140‑1 (MQ=255)                                                                                                            aGCCAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTa                          <  2:256983/140‑1 (MQ=255)                                                                                                              ccAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGaa                               <  1:63817/133‑1 (MQ=255)                                                                                                              ccAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGaa                               >  2:63817/1‑133 (MQ=255)                                                                                                              ccAGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATg                        >  1:487396/1‑140 (MQ=255)                                                                                                                aGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATggg                      <  1:463727/140‑1 (MQ=255)                                                                                                                aGGTGTTCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATggg                      <  2:653870/140‑1 (MQ=255)                                                                                                                      tCTGCTACATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTaaa                >  1:277068/1‑140 (MQ=255)                                                                                                                             cATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCg         >  2:630515/1‑140 (MQ=255)                                                                                                                             cATATTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCg         >  2:411011/1‑140 (MQ=255)                                                                                                                               tatTCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGAt       <  1:163996/140‑1 (MQ=255)                                                                                                                                 ttCGGCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGATCa     <  1:807911/140‑1 (MQ=255)                                                                                                                                    ggCGCCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGATCAggg  >  2:88617/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         GCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGATCAGGG  >  NC_000913/3926280‑3926550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑