Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,168	A→G	Y5C (TAC→TGC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,168	0	A	G	100.0%	124.9 / NA	36	Y5C (TAC→TGC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/16);  total (20/16)

TAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCG  >  NC_000913/3927041‑3927301                                                                                                                                |                                                                                                                                      tAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCA‑‑TGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAaacaac                                                                                                                         <  2:217867/140‑1 (MQ=255)   gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAaacaac                                                                                                                         <  2:682068/140‑1 (MQ=255)   gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAaacaac                                                                                                                         >  1:567241/1‑140 (MQ=255)    ccTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACg                                                                                                                        >  1:802897/1‑140 (MQ=255)             ttAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAg                                                                                                               <  1:782881/140‑1 (MQ=255)                     tCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGa                                                                                                       >  1:781862/1‑140 (MQ=255)                              aGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAGATCTCACt                                                                                              >  2:569357/1‑140 (MQ=255)                                        tttATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCa                                                                                    >  2:516427/1‑140 (MQ=255)                                        tttATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCa                                                                                    >  2:243114/1‑140 (MQ=255)                                                aTGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGgaag                                                                            <  2:6502/140‑1 (MQ=255)                                                aTGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGgaag                                                                            <  1:526546/140‑1 (MQ=255)                                                        tGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAGATCTCACTTTTCTCGTCAACTGGAAGAACGTCTg                                                                    <  1:569357/140‑1 (MQ=255)                                                            tgtgtgTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGc                                                                >  2:543699/1‑140 (MQ=255)                                                             gtgtgtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCt                                                               >  2:534866/1‑140 (MQ=255)                                                               gtgtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGa                                                             >  1:428216/1‑140 (MQ=255)                                                                  tCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCg                                                          >  2:640861/1‑140 (MQ=255)                                                                  tCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCg                                                          >  1:515462/1‑140 (MQ=255)                                                                  tCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCg                                                          >  1:141375/1‑140 (MQ=255)                                                                              gCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGgcgc                                              <  1:9865/140‑1 (MQ=255)                                                                              gCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGgcgc                                              <  2:781862/140‑1 (MQ=255)                                                                              gCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGgcgc                                              <  2:428216/140‑1 (MQ=255)                                                                                        aaGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAg                                    <  1:543699/140‑1 (MQ=255)                                                                                        aaGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAg                                    <  1:331230/140‑1 (MQ=255)                                                                                              cAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCg                                            >  1:782074/1‑126 (MQ=255)                                                                                              cAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCg                                            <  2:782074/126‑1 (MQ=255)                                                                                              cAGGACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCgtgt                              >  1:135523/1‑140 (MQ=255)                                                                                                ggACGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTgg                            >  1:493800/1‑140 (MQ=255)                                                                                                   cGCAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTggggg                         >  1:731802/1‑140 (MQ=255)                                                                                                     cAGGAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGAt                       >  2:34091/1‑140 (MQ=255)                                                                                                        gAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAg                                    >  2:129104/1‑124 (MQ=255)                                                                                                        gAGTATAAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAg                                    <  1:129104/124‑1 (MQ=255)                                                                                                             taAAAAATGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCa               <  1:516427/140‑1 (MQ=255)                                                                                                                 aaaTGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGAt           <  2:637300/140‑1 (MQ=255)                                                                                                                 aaaTGAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGAt           <  2:731802/140‑1 (MQ=255)                                                                                                                     gAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACt       >  1:385033/1‑140 (MQ=255)                                                                                                                     gAAAACCGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACt       >  1:329561/1‑140 (MQ=255)                                                                                                                          ccGCTTGCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGATCGTCTGGGGCTGATCGAAGTCCAGGCGCCTATTCTTTGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCg  <  2:802897/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      TAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCG  >  NC_000913/3927041‑3927301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑