Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,066,018	A→C	F393V (TTC→GTC)	yihP ←	putative 2,3‑dihydroxypropane‑1‑sulfonate export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,066,018	0	A	C	100.0%	308.9 / NA	86	F393V (TTC→GTC)	yihP	putative 2,3‑dihydroxypropane‑1‑sulfonate export protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/41);  total (45/41)

CAACCCATCGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGAAGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGCTGTTAACA  >  NC_000913/4065882‑4066154                                                                                                                                         |                                                                                                                                         cAACCCATCGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGgacgaa                                                                                                                                       >  2:456685/1‑140 (MQ=255)   aCCCATCGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAAc                                                                                                                                     >  1:560882/1‑140 (MQ=255)       aTCGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAAccagc                                                                                                                                 <  1:16332/140‑1 (MQ=255)         cGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAAccagcca                                                                                                                               <  1:502285/140‑1 (MQ=255)         cGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAAccagcca                                                                                                                               <  1:359814/140‑1 (MQ=255)          gCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAAccagccag                                                                                                                              >  2:16475/1‑140 (MQ=255)          gCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAAccagccag                                                                                                                              <  2:651405/140‑1 (MQ=255)             acaATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAgcgc                                                                                                                           <  1:36617/140‑1 (MQ=255)                   gTGACTCCCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTgaga                                                                                                                     <  2:441089/140‑1 (MQ=255)                   gTGACTACCGTCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTgaga                                                                                                                     <  2:496012/140‑1 (MQ=255)                   gTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTgaga                                                                                                                     <  1:42552/140‑1 (MQ=255)                   gTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTgaga                                                                                                                     >  2:761211/1‑140 (MQ=255)                      aCTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGacac                                                                                                                  >  2:744736/1‑140 (MQ=255)                           cGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGc                                                                                                             >  1:446857/1‑140 (MQ=255)                           cGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCAAGCGCCTGAGACACTTTGc                                                                                                             >  1:41029/1‑140 (MQ=255)                                 tGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAaaga                                                                                                       >  1:655117/1‑140 (MQ=255)                                  gcgcTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAaagaa                                                                                                      >  2:553374/1‑140 (MQ=255)                                         ggTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTaaaa                                                                                               <  1:704848/140‑1 (MQ=255)                                         ggTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTaaaa                                                                                               <  2:676908/140‑1 (MQ=255)                                         ggTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTaaaa                                                                                               <  2:133036/140‑1 (MQ=255)                                           tAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAAcc                                                                                             <  1:768238/140‑1 (MQ=255)                                              aTGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGgt                                                                                          >  1:346840/1‑140 (MQ=255)                                                  aGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAg                                                                                      >  1:344726/1‑140 (MQ=255)                                                  aGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAg                                                                                      <  2:113456/140‑1 (MQ=255)                                                    aTCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGAc                                                                                    >  2:288701/1‑140 (MQ=255)                                                        aCTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGtt                                                                                >  2:7103/1‑140 (MQ=255)                                                        aCTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGtt                                                                                >  2:598226/1‑140 (MQ=255)                                                              gTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGTCGAAACCAGCCAGCGCCTGAGAAACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGttccttcc                                                                          >  1:152127/1‑140 (MQ=255)                                                                 aCCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGaa                                                                       >  1:715027/1‑140 (MQ=255)                                                                      tCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAacgcac                                                                  >  1:550390/1‑140 (MQ=255)                                                                      tCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAacgcac                                                                  >  2:270562/1‑140 (MQ=255)                                                                        aaTGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAacgcacgc                                                                <  1:456685/140‑1 (MQ=255)                                                                          tGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCg                                                              <  2:715027/140‑1 (MQ=255)                                                                            gtgtGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGt                                                            >  1:404284/1‑140 (MQ=255)                                                                             tgtgGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTg                                                           >  2:339477/1‑140 (MQ=255)                                                                             tgtgGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTg                                                           >  2:166817/1‑140 (MQ=255)                                                                              gtgGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCAAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTgc                                                          <  2:41029/140‑1 (MQ=255)                                                                               tgGTCAGCCTGTGCGACGTTCTGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTgcg                                                         >  2:228342/1‑140 (MQ=255)                                                                               tgGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGTGACACTTTGCGAAAGAAGGTAAAACCGGGGAAGACCGTTCCTTCCGAACACACGCCGGTgcg                                                         >  2:534706/1‑140 (MQ=255)                                                                                 gTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGcc                                                       >  2:590263/1‑140 (MQ=255)                                                                                 gTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGcc                                                       >  1:187679/1‑140 (MQ=255)                                                                                 gTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGcc                                                       >  2:173604/1‑140 (MQ=255)                                                                                  tCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCa                                                      >  1:483525/1‑140 (MQ=255)                                                                                  tCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCa                                                      <  2:296601/140‑1 (MQ=255)                                                                                      ccTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGGAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCg                                                  >  2:706861/1‑140 (MQ=255)                                                                                      ccTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGTTGCGCCACTCg                                                  >  2:593397/1‑140 (MQ=255)                                                                                      ccTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCg                                                  <  1:553374/140‑1 (MQ=255)                                                                                       cTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTTCGCCACTCGc                                                 >  2:61343/1‑140 (MQ=255)                                                                                       cTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGc                                                 >  2:379571/1‑140 (MQ=255)                                                                                          tgCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAGGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGt                                              >  1:799791/1‑140 (MQ=255)                                                                                          tgCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGt                                              >  2:204706/1‑140 (MQ=255)                                                                                          tgCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGGAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGt                                              >  2:755156/1‑140 (MQ=255)                                                                                                gTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGa                                        <  2:446857/140‑1 (MQ=255)                                                                                                gTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGa                                        <  1:16475/140‑1 (MQ=255)                                                                                                gTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGa                                        <  1:598226/140‑1 (MQ=255)                                                                                                gTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGa                                        <  1:706861/140‑1 (MQ=255)                                                                                                   cGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAACACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACgg                                     <  2:560882/140‑1 (MQ=255)                                                                                                   cGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACgg                                     <  2:404284/140‑1 (MQ=255)                                                                                                   cGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACgg                                     <  1:204706/140‑1 (MQ=255)                                                                                                   cGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACgg                                     <  1:57704/140‑1 (MQ=255)                                                                                                    ggCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGgt                                    >  2:781295/1‑140 (MQ=255)                                                                                                           aGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGaa                             >  2:385771/1‑140 (MQ=255)                                                                                                           aGCCAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGaa                             >  2:164584/1‑140 (MQ=255)                                                                                                             ccAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAAc                           <  1:288701/140‑1 (MQ=255)                                                                                                             ccAATTTGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAAc                           >  1:561468/1‑140 (MQ=255)                                                                                                                   tGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAgcgc                     >  2:349744/1‑140 (MQ=255)                                                                                                                   tGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAgcgc                     >  1:440443/1‑140 (MQ=255)                                                                                                                   tGCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAgcgc                     <  2:626767/140‑1 (MQ=255)                                                                                                                    gCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGcc                    <  1:166817/140‑1 (MQ=255)                                                                                                                    gCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGcc                    <  1:761211/140‑1 (MQ=255)                                                                                                                    gCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGcc                    <  2:550390/140‑1 (MQ=255)                                                                                                                    gCGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGcc                    <  1:339477/140‑1 (MQ=255)                                                                                                                     cGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGccc                   >  2:565535/1‑140 (MQ=255)                                                                                                                     cGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGccc                   >  2:329067/1‑140 (MQ=255)                                                                                                                     cGTCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGccc                   <  2:440443/140‑1 (MQ=255)                                                                                                                       tCAGCATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAg                 >  2:651644/1‑140 (MQ=255)                                                                                                                           cATCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCa             >  2:416725/1‑140 (MQ=255)                                                                                                                             tCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAgg           <  2:492148/140‑1 (MQ=255)                                                                                                                             tCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAgg           >  1:358403/1‑140 (MQ=255)                                                                                                                             tCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAgg           >  1:316663/1‑140 (MQ=255)                                                                                                                             tCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAgg           <  2:299308/140‑1 (MQ=255)                                                                                                                             tCCAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAgg           <  1:597087/140‑1 (MQ=255)                                                                                                                              ccAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGc          <  1:439280/140‑1 (MQ=255)                                                                                                                              ccAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGc          <  1:270562/140‑1 (MQ=255)                                                                                                                              ccAGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGc          <  1:349744/140‑1 (MQ=255)                                                                                                                                aGCCGGGGACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGCTg        <  2:344726/140‑1 (MQ=255)                                                                                                                                     gggACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGCTGtaaca   >  2:54849/1‑136 (MQ=255)                                                                                                                                      ggACGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGCTGTTAACa  <  1:565535/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CAACCCATCGCCACAATGGTGACTACCGCCAGTGCGCTTGGGTAGATGAAGATCAACTGGCGTAACCCTTCAATGGTGTGGTCAGCCTGTGCGACGTTCGGCACATAGCCAATTTGCGTCAGCATCCAGCCGGGGAAGAAACCAGCCAGCGCCTGAGACACTTTGCGAAAGAAGGTAAAACCGGTGTAGACCGTTCCTTCCGAACGCACGCCGGTGCGCCACTCGCCGTACTCGACGGTGTCGGAAACCAGCGCCCAGTTCAGGCTGTTAACA  >  NC_000913/4065882‑4066154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑