Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,436,234	A→G	V111A (GTC→GCC)	cpdB ←	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,436,234	0	A	G	100.0%	209.9 / NA	59	V111A (GTC→GCC)	cpdB	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/33);  total (26/33)

AATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCA  >  NC_000913/4436100‑4436369                                                                                                                                       |                                                                                                                                        aaTACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGta                                                                                                                                    >  2:101609/1‑140 (MQ=255) aaTACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCAGGCGGta                                                                                                                                    >  2:49608/1‑140 (MQ=255)   tACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGtata                                                                                                                                  >  1:311483/1‑140 (MQ=255)   tACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGtata                                                                                                                                  >  1:570428/1‑140 (MQ=255)      ggTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTc                                                                                                                               >  1:692085/1‑140 (MQ=255)      ggTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTc                                                                                                                               >  2:516821/1‑140 (MQ=255)        tgtAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCa                                                                                                                             <  2:250181/140‑1 (MQ=255)        tgtAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCa                                                                                                                             <  1:64378/140‑1 (MQ=255)          tAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGt                                                                                                                                >  2:242002/1‑135 (MQ=255)          tAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGt                                                                                                                                <  1:242002/135‑1 (MQ=255)             aCATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTa                                                                                                                        >  2:84230/1‑140 (MQ=255)                 tGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATGAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTa                                                                                                                    <  1:78269/140‑1 (MQ=255)                 tGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTa                                                                                                                    <  1:101609/140‑1 (MQ=255)                 tGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTa                                                                                                                    >  2:247049/1‑140 (MQ=255)                  ggCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTaa                                                                                                                   >  2:89849/1‑140 (MQ=255)                  ggCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTaa                                                                                                                   >  1:368939/1‑140 (MQ=255)                  ggCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTaa                                                                                                                   >  1:156694/1‑140 (MQ=255)                  ggCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTa                                                                                                                    <  1:601683/139‑1 (MQ=255)                  ggCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTa                                                                                                                    >  2:601683/1‑139 (MQ=255)                   gCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  <  2:497021/140‑1 (MQ=255)                   gCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  <  1:770572/140‑1 (MQ=255)                   gCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  <  1:89849/140‑1 (MQ=255)                   gCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  <  1:243950/140‑1 (MQ=255)                    cTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  <  1:782939/139‑1 (MQ=255)                    cTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  >  2:782939/1‑139 (MQ=255)                         tGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  <  1:711560/134‑1 (MQ=255)                         tGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAAt                                                                                                                  >  2:711560/1‑134 (MQ=255)                              cTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGAc                                                                                                       <  2:763123/140‑1 (MQ=255)                                 gCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCgg                                                                                                    <  2:570428/140‑1 (MQ=255)                                  cGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCggg                                                                                                   <  2:156694/140‑1 (MQ=255)                                     cAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCgg                                                                                                    <  1:452588/136‑1 (MQ=255)                                     cAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCgg                                                                                                    >  2:452588/1‑136 (MQ=255)                                     cAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGa                                                                                                >  2:654831/1‑140 (MQ=255)                                             ttGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCAcc                                                                                        <  2:692085/140‑1 (MQ=255)                                             ttGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCAcc                                                                                        <  2:194590/140‑1 (MQ=255)                                               ggCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAatat                                                                                            >  1:599332/1‑134 (MQ=255)                                               ggCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAatat                                                                                            <  2:599332/134‑1 (MQ=255)                                                         tAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATcc                                                                            <  2:735135/140‑1 (MQ=255)                                                             ggAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTc                                                                        >  2:32835/1‑140 (MQ=255)                                                               aaTTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGc                                                                      <  2:311483/140‑1 (MQ=255)                                                                                   aTTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCg                                                  <  2:678495/140‑1 (MQ=255)                                                                                     ttttCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGa                                                <  2:368939/140‑1 (MQ=255)                                                                                          aGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTccc                                           >  1:546431/1‑140 (MQ=255)                                                                                               aTCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGTCCAGCGGACTCCCCTGaa                                      >  1:625311/1‑140 (MQ=255)                                                                                               aTCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGaa                                      >  2:727581/1‑140 (MQ=255)                                                                                                   aGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCaa                                  <  1:247049/140‑1 (MQ=255)                                                                                                    gACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCaaa                                 <  2:425462/140‑1 (MQ=255)                                                                                                      ccGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATc                               >  2:786635/1‑140 (MQ=255)                                                                                                        gTAGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc                             <  1:786635/140‑1 (MQ=255)                                                                                                          aGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCg                           >  2:138733/1‑140 (MQ=255)                                                                                                          aGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCg                           <  1:727581/140‑1 (MQ=255)                                                                                                          aGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCg                           <  1:84230/140‑1 (MQ=255)                                                                                                          aGTTAAACTCGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACCCCCCTGAATCAAATCGCCg                           >  2:699440/1‑140 (MQ=255)                                                                                                                   cGTGGTTGCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAAcc                  <  1:444889/140‑1 (MQ=255)                                                                                                                       gTTGCCAAGCGTTCAGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTa              <  1:49608/140‑1 (MQ=255)                                                                                                                          gCCAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGc           >  2:450042/1‑140 (MQ=255)                                                                                                                            cAAGCGTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg         <  1:138733/140‑1 (MQ=255)                                                                                                                                 gTTCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt    <  2:288099/140‑1 (MQ=255)                                                                                                                                   tCCGGCGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCa  <  1:32835/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCA  >  NC_000913/4436100‑4436369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑