| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 372,113 | T→C | intergenic (+8/‑2) | mhpC → / → mhpD | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase/2‑hydroxypentadienoate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 372,113 | 0 | T | C | 97.7% | 148.6 / ‑4.4 | 44 | intergenic (+8/‑2) | mhpC/mhpD | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase/2‑hydroxypentadienoate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (23/20); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAAT > NC_000913/371978‑372249 | gtctgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATg > 2:370448/1‑140 (MQ=255) tgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACg > 2:596236/1‑140 (MQ=255) tgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACg < 1:734832/140‑1 (MQ=255) ctgctgTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCa < 1:622361/140‑1 (MQ=255) gctgTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGAc < 1:790539/132‑1 (MQ=255) gctgTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGAc > 2:790539/1‑132 (MQ=255) gctgTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATa < 1:787706/140‑1 (MQ=255) cGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGaa < 1:73066/129‑1 (MQ=255) cGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGaa > 2:73066/1‑129 (MQ=255) ccGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTg > 2:690786/1‑140 (MQ=255) ccGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTg > 2:756447/1‑140 (MQ=255) cATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcg > 1:53414/1‑140 (MQ=255) gcgACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCa > 2:638172/1‑140 (MQ=255) gcgACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCa > 2:309510/1‑140 (MQ=255) tgGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAgg > 2:505897/1‑140 (MQ=255) ggCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGa < 2:169803/140‑1 (MQ=255) gcAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTg > 1:42131/1‑140 (MQ=255) cAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGc > 2:211936/1‑140 (MQ=255) gggAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCg < 2:352192/140‑1 (MQ=255) tGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGcgcg > 2:685359/1‑140 (MQ=255) ccGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGAt < 2:469721/140‑1 (MQ=255) aCGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTg > 2:252426/1‑140 (MQ=255) cGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa > 1:650208/1‑140 (MQ=255) cGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa < 1:505897/140‑1 (MQ=255) cGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa < 1:309510/140‑1 (MQ=255) cGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa < 1:211936/140‑1 (MQ=255) cGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGa < 2:650208/140‑1 (MQ=255) tcaatcaaCTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGt < 1:199782/140‑1 (MQ=255) tcaatcaaCTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGt < 2:87808/140‑1 (MQ=255) caaCTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGa < 1:596236/140‑1 (MQ=255) caaCTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGa < 2:8296/140‑1 (MQ=255) cTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATaa > 2:129533/1‑140 (MQ=255) cTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGa > 2:252205/1‑131 (MQ=255) cTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGa < 1:252205/131‑1 (MQ=255) cTGAATTTCCTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGa > 1:145838/1‑140 (MQ=255) ccTCGCACGCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCtt > 1:485657/1‑140 (MQ=255) gCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCAtt < 2:145838/140‑1 (MQ=255) gCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCAtt < 1:685359/140‑1 (MQ=255) gCCCTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCAtt < 1:129533/140‑1 (MQ=255) ccTTAAGGAATGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCa < 2:42131/140‑1 (MQ=255) aaTGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATaa > 1:151058/1‑140 (MQ=255) tGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAAt > 2:672502/1‑140 (MQ=255) tGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAAt > 1:78244/1‑140 (MQ=255) tGGCCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAAt > 1:659847/1‑140 (MQ=255) | GTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAAT > NC_000913/371978‑372249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |