Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	373,630	T→C	V237A (GTA→GCA)	mhpF →	acetaldehyde dehydrogenase (acylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	373,630	0	T	C	100.0%	91.7 / NA	29	V237A (GTA→GCA)	mhpF	acetaldehyde dehydrogenase (acylating)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/14);  total (15/14)

GGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTAT  >  NC_000913/373496‑373766                                                                                                                                       |                                                                                                                                         ggCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCgg                                                                                                                                     >  2:174039/1‑140 (MQ=255)                      agcagAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGcgcg                                                                                                                >  2:272998/1‑140 (MQ=255)                           agCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTg                                                                                                              <  2:477465/137‑1 (MQ=255)                           agCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTg                                                                                                              >  1:477465/1‑137 (MQ=255)                             ccACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGtt                                                                                                         <  2:780613/140‑1 (MQ=255)                              cACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGttt                                                                                                        >  1:106755/1‑140 (MQ=255)                                  gTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAg                                                                                                    <  2:51509/140‑1 (MQ=255)                                     gatgatGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTCCGCACCGGGTTATCGCCTGAAAAAGCGCGTGCCGTTTTAAGTTa                                                                                                 >  2:772105/1‑140 (MQ=255)                                         atgCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATccc                                                                                             <  1:174039/140‑1 (MQ=255)                                          tgCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCg                                                                                            <  2:583364/140‑1 (MQ=255)                                                acacGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGAt                                                                                      >  1:308739/1‑140 (MQ=255)                                                     gtgtATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGGTATCGCCTGAAACAGCGCGGGCAGTTTGAAGTTATCCCGCCGGATaaaac                                                                                 >  2:397752/1‑138 (MQ=255)                                                                gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTAc                                                                      >  1:746888/1‑140 (MQ=255)                                                                gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTAc                                                                      >  2:56687/1‑140 (MQ=255)                                                                gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTAc                                                                      >  2:56669/1‑140 (MQ=255)                                                                gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTAc                                                                      >  2:790404/1‑140 (MQ=255)                                                                      cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCTGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCg                                                                >  1:315967/1‑140 (MQ=255)                                                                      cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCg                                                                >  1:124926/1‑140 (MQ=255)                                                                             tCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCa                                                         <  2:106755/140‑1 (MQ=255)                                                                                   gatgatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGGCAATTTACCGGGCGTGGGGCAATtctc                                                   >  2:477262/1‑140 (MQ=255)                                                                                        tatCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCTGGCGTGGGGCAATTCTCCGGAc                                              >  1:383156/1‑140 (MQ=255)                                                                                                 cTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAg                                     >  1:654150/1‑140 (MQ=255)                                                                                                          tGAAATGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTctggc                            >  1:290427/1‑140 (MQ=255)                                                                                                              aTGGCTGAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGa                        <  2:195615/140‑1 (MQ=255)                                                                                                                    gAGGCGGTGCAGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGa                  <  2:469441/140‑1 (MQ=255)                                                                                                                              aGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgcagcg        <  2:315967/140‑1 (MQ=255)                                                                                                                              aGGCTTACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgcagcg        <  1:790404/140‑1 (MQ=255)                                                                                                                                  ttACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCAtt    <  2:308739/140‑1 (MQ=255)                                                                                                                                  ttACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCAtt    <  2:290427/140‑1 (MQ=255)                                                                                                                                  ttACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCAtt    >  2:655592/1‑140 (MQ=255)                                                                                                                                  ttACGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCAtt    <  1:397752/140‑1 (MQ=255)                                                                                                                                    aCGCACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTAt  <  2:46241/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTAT  >  NC_000913/373496‑373766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑