Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,933	C→T	D142N (GAT→AAT)  S35L (TCG→TTG)	yaaW ← mbiA →	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,933	0	C	T	100.0%	163.3 / NA	46	D142N (GAT→AAT)  S35L (TCG→TTG)	yaaW mbiA	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

CCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAA  >  NC_000913/10797‑11069                                                                                                                                         |                                                                                                                                         ccAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGAc                                                                                                                                       >  2:280285/1‑140 (MQ=255)   aaGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACcg                                                                                                                                     <  2:394391/140‑1 (MQ=255)     gTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACcgcg                                                                                                                                   <  2:74417/140‑1 (MQ=255)        cGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGc                                                                                                                                >  1:710649/1‑140 (MQ=255)          cTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACcgcg                                                                                                                                   >  1:496224/1‑135 (MQ=255)          cTCATTGCTGCGTGGGTGCGTAAAAAGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACcgcg                                                                                                                                   <  2:496224/135‑1 (MQ=255)              ttGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAAc                                                                                                                          <  1:397984/140‑1 (MQ=255)              ttGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAAc                                                                                                                          <  1:600006/140‑1 (MQ=255)               tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACt                                                                                                                         <  1:504256/140‑1 (MQ=255)                      tGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCtt                                                                                                                  >  2:346662/1‑140 (MQ=255)                       gggTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTa                                                                                                                 >  1:111960/1‑140 (MQ=255)                        ggTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACt                                                                                                                         >  1:444483/1‑131 (MQ=255)                        ggTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACt                                                                                                                         <  2:444483/131‑1 (MQ=255)                         gTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATg                                                                                                               >  1:554363/1‑140 (MQ=255)                           gCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGtt                                                                                                             >  1:143164/1‑140 (MQ=255)                            cGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTc                                                                                                            >  1:751618/1‑140 (MQ=255)                            cGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTc                                                                                                            >  1:619432/1‑140 (MQ=255)                              tAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGGGCAGAAACTCCTGCTTATGTTCCt                                                                                                          >  2:13268/1‑140 (MQ=255)                                 aaTGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGt                                                                                                       <  2:492537/140‑1 (MQ=255)                                      gggTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAtt                                                                                                  <  1:13268/140‑1 (MQ=255)                                      gggTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAtt                                                                                                  <  1:698231/140‑1 (MQ=255)                                                gCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAgg                                                                                        <  2:710649/140‑1 (MQ=255)                                                       aGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTcc                                                                                           >  1:355397/1‑130 (MQ=255)                                                       aGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTcc                                                                                           <  2:355397/130‑1 (MQ=255)                                                       aGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACg                                                                                 >  1:168658/1‑140 (MQ=255)                                                            aTGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGaa                                                                            >  2:522218/1‑140 (MQ=255)                                                                 acacacCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGt                                                                       <  2:490150/140‑1 (MQ=255)                                                                       cTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGt                                                                 <  2:619432/140‑1 (MQ=255)                                                                       cTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTACTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGt                                                                 <  2:751618/140‑1 (MQ=255)                                                                                 taatTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCt                                                       >  1:680968/1‑140 (MQ=255)                                                                                 taatTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCt                                                       >  2:579670/1‑140 (MQ=255)                                                                                           ttCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCaaa                                             <  1:579670/140‑1 (MQ=255)                                                                                                      cGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACAtct                                  >  2:568463/1‑140 (MQ=255)                                                                                                      cGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACAtct                                  >  2:768985/1‑140 (MQ=255)                                                                                                      cGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACAtct                                  >  1:622732/1‑140 (MQ=255)                                                                                                      cGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACAtct                                  <  1:280285/140‑1 (MQ=255)                                                                                                      cGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTGATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACAtct                                  >  2:560722/1‑140 (MQ=255)                                                                                                        gcagcaGCTCTTCCAGCTCATTCACCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATctct                                >  1:584985/1‑140 (MQ=255)                                                                                                                               aCCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCg         <  2:111960/140‑1 (MQ=255)                                                                                                                               aCCCTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCg         <  1:181844/140‑1 (MQ=255)                                                                                                                                 ccTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCg         <  2:471007/138‑1 (MQ=255)                                                                                                                                 ccTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCg         >  1:471007/1‑138 (MQ=255)                                                                                                                                 ccTGGCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCt       <  2:498291/140‑1 (MQ=255)                                                                                                                                     gCATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGa   >  1:423891/1‑140 (MQ=255)                                                                                                                                      cATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGaa  <  2:584985/140‑1 (MQ=255)                                                                                                                                      cATTGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGaa  <  2:622732/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAA  >  NC_000913/10797‑11069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑