Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	388,324	C→T	A177V (GCG→GTG)	tauD →	taurine dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	388,324	0	C	T	100.0%	115.4 / NA	39	A177V (GCG→GTG)	tauD	taurine dioxygenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/6);  total (33/6)

TTGCGGCCTATGAGGCGCTCTCTGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGT  >  NC_000913/388189‑388458                                                                                                                                        |                                                                                                                                       ttGCGGCCTATGAGGCGCTCTCTGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTc                                                                                                                                    >  1:245563/1‑140 (MQ=255) ttGCGGCCTATGAGGCGCTCTCTGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTc                                                                                                                                    >  1:789089/1‑140 (MQ=255)      gCCTATGAGGCGCTCTCTGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGaa                                                                                                                               <  1:220296/140‑1 (MQ=255)           tGAGGCGCTCTCTGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACTCTGGCGCGAGGTGGTCGCGAAAAAcc                                                                                                                          >  1:567531/1‑140 (MQ=255)               gcgcTCTCTGTCCCCTCCCGCCAGCTGCTGAGGGGGCTGCGTGCGGAGCATGATTCCCGAAAATCGTCCCCGGAATACAAATCCCCAAAAACCGAGGAGAAACATCACCGCGGGCGCGAGGTGGTCGCAAAAAACccgcc                                                                                                                      <  2:367443/140‑1 (MQ=255)                     tctGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGAAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCt                                                                                                                >  2:81138/1‑140 (MQ=255)                            ccTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCg                                                                                                         >  2:636411/1‑140 (MQ=255)                             cTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCgg                                                                                                        >  2:96268/1‑140 (MQ=255)                             cTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCgg                                                                                                        >  1:64687/1‑140 (MQ=255)                             cTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCgg                                                                                                        >  1:646076/1‑140 (MQ=255)                               tCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCggtg                                                                                                      >  2:42511/1‑140 (MQ=255)                               tCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCggtg                                                                                                      >  1:771412/1‑140 (MQ=255)                               tCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCggtg                                                                                                      >  1:457738/1‑140 (MQ=255)                               tCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGACGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCggtg                                                                                                      >  1:590132/1‑140 (MQ=255)                                 cGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCggtggg                                                                                                    >  2:57335/1‑139 (MQ=255)                                   ccAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGc                                                                                                  >  1:543612/1‑140 (MQ=255)                                      gctgctGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTCCCCGGAATACAAATCCCACAAACCCGAGGAGGAACATCAACGCGGGCGCGAGGTGGTCGCAAAAAACCCGCCGTTGCTACATCCGGTGGTGCGaa                                                                                               <  2:592130/140‑1 (MQ=255)                                        tgctgAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGGGAGGTGGTCGCAAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACg                                                                                             <  2:524857/140‑1 (MQ=255)                                          ctgAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCa                                                                                           >  1:420775/1‑140 (MQ=255)                                           ggggTGGGCTGCGTGGGGAGCATGTTTTCCGTAAATCTTCCCCGGAATAAAAATACCGCAAACCCGAGGAGGAACATCAACGCGGGCGCGAGGTGGTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCAt                                                                                          <  2:543612/137‑1 (MQ=255)                                                 ggCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTTCTACATCCGGTGGTGCGAACGCATCCGGTg                                                                                    >  2:496971/1‑140 (MQ=255)                                                 ggCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTg                                                                                    >  1:235291/1‑140 (MQ=255)                                                    tgcgtgcgGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGc                                                                                 >  1:330550/1‑140 (MQ=255)                                                    tgcgtgcgGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGc                                                                                 >  2:218454/1‑140 (MQ=255)                                                             aGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAg                                                                        >  2:781650/1‑140 (MQ=255)                                                                gtGATTTCCGTAAATCGTTCCCGAAATACAAAAACCGCAAACCCGAGGAGAATCATCAACGTGGGCGCGGGGTGGTCGGGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGgcg                                                                     <  2:646076/139‑1 (MQ=255)                                                                aTGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGgcg                                                                     <  1:41939/140‑1 (MQ=255)                                                                aTGATTTCCGTAAATCGTCCCCGGAATACAAATACCGAAAAACCGAGGAGGAACATCAACGCGGGCGCGGGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGgcg                                                                     <  2:276438/140‑1 (MQ=255)                                                                             aTCGTCCCCGGAATAAAAATCCCGCAAAACCGAGGAGAAACATCACCGCTGGCGCGAGGTGTTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGtgaatg                                                        <  2:235291/140‑1 (MQ=255)                                                                             aTCGTCCCCGAAATAAAAATCCCGCAAAACCGAGGAGGAACATCAACGCGGGCGCGAGGTGGTCGCAAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGtgaatg                                                        <  2:420775/140‑1 (MQ=255)                                                                                 ttCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAgg                                                    >  1:792582/1‑140 (MQ=255)                                                                                  tCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGc                                                   >  1:41561/1‑140 (MQ=255)                                                                                             aaaaACCGCAAACCCGAGGAGGAACATAAGCGCTGGCGGGGGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTAcgcg                                        <  2:265471/140‑1 (MQ=255)                                                                                             aaaTACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTAcgcg                                        <  1:218123/140‑1 (MQ=255)                                                                                             aaaTACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTAcgcg                                        <  1:781650/140‑1 (MQ=255)                                                                                             aaaTACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTAcgcg                                        <  1:218454/140‑1 (MQ=255)                                                                                               atcccGAAAACCGGAGGAGGAACATCAACGCGGGGGGGGGGTGGTCGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGaa                                      <  2:64687/137‑1 (MQ=255)                                                                                                  ccGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAAttg                                   >  2:416338/1‑140 (MQ=255)                                                                                                  ccGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAAttg                                   >  2:772797/1‑140 (MQ=255)                                                                                                    gCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAAttgtt                                 >  1:343423/1‑140 (MQ=255)                                                                                                     cAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAAttgttg                                >  1:331681/1‑140 (MQ=255)                                                                                                      aaaaCCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGa                               >  2:627150/1‑140 (MQ=255)                                                                                                      aaaaCCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGa                               >  1:180511/1‑140 (MQ=255)                                                                                                      aaaaCCGAGGAGGAACATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGa                               >  1:620646/1‑140 (MQ=255)                                                                                                                gggaACATCAACGCGGGCGGGGGGTGGCCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCgag                     <  2:771412/139‑1 (MQ=255)                                                                                                                   aaCATCACCGCGGGCGGGAGGTGGTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGaaa                  <  2:41561/140‑1 (MQ=255)                                                                                                                   aaCATCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGaaa                  <  1:81138/140‑1 (MQ=255)                                                                                                                       tCAACGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAagaga              >  2:619864/1‑140 (MQ=255)                                                                                                                          aCGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCg           <  1:96268/140‑1 (MQ=255)                                                                                                                          aCGCTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCg           >  1:167022/1‑140 (MQ=255)                                                                                                                             ggggCGTGAGGTGGCGGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAg        <  1:42511/138‑1 (MQ=255)                                                                                                                             cTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTTCATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAg        >  1:651656/1‑140 (MQ=255)                                                                                                                             cTGGCGCGAGGTGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAg        >  2:296607/1‑140 (MQ=255)                                                                                                                                   ggAGGTGGTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGt  <  2:330550/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TTGCGGCCTATGAGGCGCTCTCTGTTCCCTTCCGCCAGCTGCTGAGTGGGCTGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGT  >  NC_000913/388189‑388458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑