Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	523,138	T→C	intergenic (+308/‑123)	ybbP → / → rhsD	putative ABC transporter membrane subunit YbbP/protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	523,138	0	T	C	100.0%	133.3 / NA	38	intergenic (+308/‑123)	ybbP/rhsD	putative ABC transporter membrane subunit YbbP/protein RhsD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/24);  total (14/24)

ACTAATAAAATCATAAATCATATGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGTTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAA  >  NC_000913/523006‑523272                                                                                                                                     |                                                                                                                                        aCTAATAAAATCATAAATCATATGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGctgattga                                                                                                                                  >  2:15228/1‑140 (MQ=255)           tcataaatcataTGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATaa                                                                                                                        >  2:277824/1‑140 (MQ=255)                     tatGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGa                                                                                                              >  1:238513/1‑140 (MQ=255)                     tatGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGa                                                                                                              >  1:260774/1‑140 (MQ=255)                      atGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGaa                                                                                                             <  1:343306/140‑1 (MQ=255)                       tGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAGCAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGaaa                                                                                                            >  2:546330/1‑140 (MQ=255)                       tGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGCGaaa                                                                                                            <  2:138642/140‑1 (MQ=255)                        gCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGaaaa                                                                                                           <  2:463993/140‑1 (MQ=255)                           ttGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAata                                                                                                        >  2:363624/1‑140 (MQ=255)                               aTGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAGCAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAAtatata                                                                                                    <  1:546330/140‑1 (MQ=255)                                   gtattacGCATATAGGGAGTTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGCAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATa                                                                                                >  2:114207/2‑140 (MQ=255)                                     attatCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACa                                                                                              <  2:573011/140‑1 (MQ=255)                                     attatCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACa                                                                                              <  2:405322/140‑1 (MQ=255)                                        atCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTa                                                                                           >  2:595106/1‑140 (MQ=255)                                         tCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAg                                                                                          <  2:257157/140‑1 (MQ=255)                                             tataGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTgg                                                                                      >  2:622625/1‑140 (MQ=255)                                                  tGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTtg                                                                                 >  2:430300/1‑140 (MQ=255)                                                    aaTTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTtgtg                                                                               >  1:425243/1‑140 (MQ=255)                                                    aaTTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTtgtg                                                                               >  1:253513/1‑140 (MQ=255)                                                            gatgatgaATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCattt                                                                       >  2:217619/1‑140 (MQ=255)                                                               gatgaATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCatttta‑t                                                                   <  2:406012/140‑1 (MQ=255)                                                                       cATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑ttttttttt                                                           <  1:75605/140‑1 (MQ=255)                                                                        aTCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTttg                                                          >  1:224956/1‑140 (MQ=255)                                                                            gtgCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGc                                                      <  2:76448/140‑1 (MQ=255)                                                                             tgCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCt                                                     <  2:604613/140‑1 (MQ=255)                                                                             tgCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCt                                                     >  2:58958/1‑140 (MQ=255)                                                                              gCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑tttttttttttgttgc                                                    <  2:425243/140‑8 (MQ=255)                                                                                  aaaaTGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAacac                                                <  2:224956/140‑1 (MQ=255)                                                                                   aaaTGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAAcaca                                               <  2:260774/140‑1 (MQ=255)                                                                                   aaaTGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAAcaca                                               <  1:573574/140‑1 (MQ=255)                                                                                      tGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAggg                                            <  1:768881/140‑1 (MQ=255)                                                                                             ttAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGaa                                     <  1:281391/140‑1 (MQ=255)                                                                                                 taaAATATTGAAAGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAAt                                 <  2:272225/140‑1 (MQ=255)                                                                                                           aaaGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAgg                       <  1:491597/140‑1 (MQ=255)                                                                                                            aaGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAggg                      <  2:238513/140‑1 (MQ=255)                                                                                                            aaGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAggg                      <  1:430300/140‑1 (MQ=255)                                                                                                            aaGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAggg                      <  1:363624/140‑1 (MQ=255)                                                                                                            aaGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAggg                      <  1:595106/140‑1 (MQ=255)                                                                                                            aaGTGACCTGTAATAACAGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAggg                      <  1:179008/140‑1 (MQ=255)                                                                                                                             aGTTGTTGCTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTATTTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCgg      >  1:269366/1‑140 (MQ=255)                                                                                                                                tgttgctgATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGaaaa  >  2:35324/1‑140 (MQ=255)                                                                                                                                tgttgctgATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGaaaa  >  2:633995/1‑140 (MQ=255)                                                                                                                                tgttgctgATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGaaaa  >  2:648833/1‑140 (MQ=255)                                                                                                                                tgttgctgATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGaaaa  >  1:136295/1‑140 (MQ=255)                                                                                                                                tgttgatgATTGAGAACAAAAAAGTTTATGTGAAAAATATATAACTACATTAGCTGGAATTGTGTGTCATTTTA‑TTCTTTTTTGTTGCCAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAACGGaaaa  >  2:13404/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ACTAATAAAATCATAAATCATATGCGTTGAATGGATATTATCCATATAGTGAATTTGTTGATGATGAATTCATCTGTGCTAAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGTTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTA‑TTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAA  >  NC_000913/523006‑523272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑