Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	602,098	C→T	P47L (CCG→CTG)	pheP →	phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	602,098	0	C	T	100.0%	198.8 / NA	56	P47L (CCG→CTG)	pheP	phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

AAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCC  >  NC_000913/601962‑602231                                                                                                                                         |                                                                                                                                      aaaaaCGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGc                                                                                                                                    >  1:391420/1‑140 (MQ=255)           cAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATg                                                                                                                          <  1:199859/140‑1 (MQ=255)            aaCCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATgg                                                                                                                         <  2:688263/140‑1 (MQ=255)                gTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCggg                                                                                                                     <  2:407552/140‑1 (MQ=255)                gTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCggg                                                                                                                     <  2:404660/140‑1 (MQ=255)                gTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCggg                                                                                                                     <  1:723518/140‑1 (MQ=255)                   tCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTcc                                                                                                                  <  2:246567/140‑1 (MQ=255)                     ggAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCgg                                                                                                                <  2:628421/140‑1 (MQ=255)                       aaGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCt                                                                                                              >  2:272748/1‑140 (MQ=255)                             cTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTg                                                                                                        >  1:421168/1‑140 (MQ=255)                             cTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTg                                                                                                        <  2:331667/140‑1 (MQ=255)                                cGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTg                                                                                                     >  1:142352/1‑140 (MQ=255)                                cGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTg                                                                                                     <  2:142352/140‑1 (MQ=255)                                 gTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTgg                                                                                                    >  2:196752/1‑140 (MQ=255)                                    gAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCt                                                                                                 >  2:513340/1‑140 (MQ=255)                                    gAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCt                                                                                                 <  1:67331/140‑1 (MQ=255)                                     aaTCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCt                                                                                                 >  1:346350/1‑139 (MQ=255)                                     aaTCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCt                                                                                                 <  2:346350/139‑1 (MQ=255)                                          agagCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCg                                                                                           >  1:600705/1‑140 (MQ=255)                                                 aCGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCgg                                                                                    >  2:206783/1‑140 (MQ=255)                                                    cTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGat                                                                                 <  2:600705/140‑1 (MQ=255)                                                         tCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCg                                                                            <  2:82838/140‑1 (MQ=255)                                                         tCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCg                                                                            >  1:178756/1‑140 (MQ=255)                                                          cgcgGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGc                                                                           <  2:433466/140‑1 (MQ=255)                                                               aTTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTcc                                                                      >  1:501191/1‑140 (MQ=255)                                                                         cGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGc                                                                           >  1:186391/1‑125 (MQ=255)                                                                         cGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGc                                                                           <  2:186391/125‑1 (MQ=255)                                                                             atatTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAg                                                        >  2:789274/1‑140 (MQ=255)                                                                                ttCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCtt                                                     >  1:25136/1‑140 (MQ=255)                                                                                     cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                >  2:68526/1‑140 (MQ=255)                                                                                     cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                >  1:497748/1‑140 (MQ=255)                                                                                     cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                >  1:481521/1‑140 (MQ=255)                                                                                     cTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGa                                                <  1:206783/140‑1 (MQ=255)                                                                                          tGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtgg                                           >  2:210068/1‑140 (MQ=255)                                                                                          tGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAAtgg                                           >  1:429232/1‑140 (MQ=255)                                                                                               tGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGtt                                      <  1:789274/140‑1 (MQ=255)                                                                                               tGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGtt                                      >  2:76745/1‑140 (MQ=255)                                                                                                 ggTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTga                                    <  1:6641/140‑1 (MQ=255)                                                                                                  gTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTgag                                   <  1:272748/140‑1 (MQ=255)                                                                                                           ttGGTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTa                          <  1:585359/140‑1 (MQ=255)                                                                                                             ggTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTgaggag                                >  1:218319/1‑132 (MQ=255)                                                                                                             ggTACTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTgaggag                                <  2:218319/132‑1 (MQ=255)                                                                                                                aCTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCgg                     >  1:165052/1‑140 (MQ=255)                                                                                                                aCTGGTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCgg                     >  2:275760/1‑140 (MQ=255)                                                                                                                   ggTCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTc                  >  1:690852/1‑140 (MQ=255)                                                                                                                     tCTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGTGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCAt                >  1:405168/1‑140 (MQ=255)                                                                                                                      cTGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCAtt               <  2:497748/140‑1 (MQ=255)                                                                                                                       tGTTTCTTGGCATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCAttt              >  1:588058/1‑140 (MQ=255)                                                                                                                                 cATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    <  1:196752/140‑1 (MQ=255)                                                                                                                                 cATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    <  2:178756/140‑1 (MQ=255)                                                                                                                                 cATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    <  1:196645/140‑1 (MQ=255)                                                                                                                                 cATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    <  2:501191/140‑1 (MQ=255)                                                                                                                                 cATTGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    <  2:481521/140‑1 (MQ=255)                                                                                                                                   ttGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    <  2:598470/138‑1 (MQ=255)                                                                                                                                   ttGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg    >  1:598470/1‑138 (MQ=255)                                                                                                                                   ttGGCCTGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGcc  >  2:293586/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCC  >  NC_000913/601962‑602231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑