Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	634,668	A→G	P361P (CCA→CCG)	ybdL →	methionine‑oxo‑acid transaminase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	634,668	0	A	G	100.0%	110.8 / NA	32	P361P (CCA→CCG)	ybdL	methionine‑oxo‑acid transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/13);  total (19/13)

GAAGGTACATACTTTTTGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCC  >  NC_000913/634534‑634803                                                                                                                                       |                                                                                                                                        gAAGGTACATACTTTTTGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATaa                                                                                                                                    >  1:562025/1‑140 (MQ=255)      tACATACTTTTTGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGa                                                                                                                               >  1:488220/1‑140 (MQ=255)        cATACTTTTTGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGAt                                                                                                                              >  1:429967/1‑139 (MQ=255)        cATACTTTTTGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGAt                                                                                                                              <  2:429967/139‑1 (MQ=255)             tttttGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGtct                                                                                                                        <  2:488220/140‑1 (MQ=255)             tttttGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGtct                                                                                                                        <  2:448418/140‑1 (MQ=255)               tttGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTctct                                                                                                                      >  2:469084/1‑140 (MQ=255)                               aGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGa                                                                                                      <  1:469084/140‑1 (MQ=255)                                 cgcgGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAAt                                                                                                    >  1:115620/1‑140 (MQ=255)                                   cgGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCg                                                                                                  >  1:197129/1‑140 (MQ=255)                                    ggTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGa                                                                                                 <  2:651001/140‑1 (MQ=255)                                      tttCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACg                                                                                               <  1:253298/140‑1 (MQ=255)                                              cTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGgc                                                                                       <  2:511357/140‑1 (MQ=255)                                                ggATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGgcag                                                                                     >  1:347187/1‑140 (MQ=255)                                                      tgTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTg                                                                               >  1:70272/1‑140 (MQ=255)                                                      tgTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTg                                                                               <  2:24720/140‑1 (MQ=255)                                                      tgTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTg                                                                               >  1:15583/1‑140 (MQ=255)                                                           aGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGc                                                                          <  1:127089/140‑1 (MQ=255)                                                                tGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTgcg                                                                     <  2:347187/140‑1 (MQ=255)                                                                tGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTgcg                                                                     >  1:240530/1‑140 (MQ=255)                                                                      tGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCt                                                               >  1:197219/1‑140 (MQ=255)                                                                          tGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAg                                                           <  2:197219/140‑1 (MQ=255)                                                                                  gAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTaa                                                   <  2:115620/140‑1 (MQ=255)                                                                                             aGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGgcg                                        >  1:773132/1‑140 (MQ=255)                                                                                               cggcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGcgcg                                      >  2:345585/1‑140 (MQ=255)                                                                                                 gcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCggg                                    >  1:439923/1‑140 (MQ=255)                                                                                                              cGGTGTTTTGCGCCGATCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTc                       >  2:191727/1‑140 (MQ=255)                                                                                                                             aTCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTg        >  1:634308/1‑140 (MQ=255)                                                                                                                             aTCCCTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTg        >  2:637771/1‑140 (MQ=255)                                                                                                                               cccTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaac                <  1:637360/130‑1 (MQ=255)                                                                                                                               cccTTCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaac                >  2:637360/1‑130 (MQ=255)                                                                                                                                   tCCCGCATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGcc  >  1:328769/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GAAGGTACATACTTTTTGCTGGTGGATTACAGCGCGGTTTCTACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCC  >  NC_000913/634534‑634803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑