Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	718,276	C→T	G729R (GGG→AGG)	speF ←	ornithine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,276	0	C	T	100.0%	159.3 / NA	45	G729R (GGG→AGG)	speF	ornithine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/26);  total (19/26)

TTGAGATTGTCCCGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCCTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGGCAGCAGGTTGATCCCTTCTTCCAG  >  NC_000913/718142‑718411                                                                                                                                       |                                                                                                                                        ttGAGATTGTCCCGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCa                                                                                                                                    >  2:109740/1‑140 (MQ=255)     gaTTGTCCCGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAg                                                                                                                                >  1:391911/1‑140 (MQ=255)     gaTTGTCCCGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAg                                                                                                                                >  1:352766/1‑140 (MQ=255)      aTTGTCCCGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAgg                                                                                                                               >  1:331301/1‑140 (MQ=255)           cccGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCt                                                                                                                          <  1:627017/140‑1 (MQ=255)                    ggCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCTACAGGGTGCTTTGCGCATc                                                                                                                 >  1:36140/1‑140 (MQ=255)                    ggCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATc                                                                                                                 >  1:124671/1‑140 (MQ=255)                      cAAGCTTTGTCGGCGGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCAc                                                                                                               <  2:625733/140‑1 (MQ=255)                      cAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCAc                                                                                                               <  2:124671/140‑1 (MQ=255)                       aaGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACg                                                                                                              <  2:539809/140‑1 (MQ=255)                        aGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGa                                                                                                             <  2:412065/140‑1 (MQ=255)                         gCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAg                                                                                                            >  2:267934/1‑140 (MQ=255)                          cTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAgg                                                                                                           <  1:123200/140‑1 (MQ=255)                          cTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAgg                                                                                                           <  2:331301/140‑1 (MQ=255)                          cTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAgg                                                                                                           <  1:35856/140‑1 (MQ=255)                          cTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAgg                                                                                                           <  2:471431/140‑1 (MQ=255)                               tCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTtga                                                                                                      <  1:185041/140‑1 (MQ=255)                                    agcaTGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACa                                                                                                 <  1:63410/140‑1 (MQ=255)                                                    aGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCt                                                                                 >  2:245183/1‑140 (MQ=255)                                                     gCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCtt                                                                                >  1:705004/1‑140 (MQ=255)                                                      cccATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTa                                                                               >  2:175332/1‑140 (MQ=255)                                                         atcatGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGa                                                                            >  1:296429/1‑140 (MQ=255)                                                              gTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACcatc                                                                       <  1:175332/140‑1 (MQ=255)                                                                   ccATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGtt                                                                  <  2:296429/140‑1 (MQ=255)                                                                     aTCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGttct                                                                <  1:240836/140‑1 (MQ=255)                                                                     aTCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGttct                                                                <  2:705004/140‑1 (MQ=255)                                                                     aTCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGttct                                                                <  2:146624/140‑1 (MQ=255)                                                                       cGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGttcttc                                                              >  1:319874/1‑140 (MQ=255)                                                                         tCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGa                                                            <  1:109740/140‑1 (MQ=255)                                                                              aTGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAg                                                       <  2:607623/140‑1 (MQ=255)                                                                                 gTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGaca                                                    >  1:474804/1‑140 (MQ=255)                                                                                 gTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGaca                                                    <  1:267934/140‑1 (MQ=255)                                                                                  ttAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGttct                                                                >  2:245366/1‑127 (MQ=255)                                                                                  ttAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGttct                                                                <  1:245366/127‑1 (MQ=255)                                                                                  ttAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGacac                                                   >  2:754498/1‑140 (MQ=255)                                                                                    aaCTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCGCCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACAccc                                                 >  2:752315/1‑140 (MQ=255)                                                                                     aCTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCt                                                >  1:298397/1‑140 (MQ=255)                                                                                         aaCGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGTTGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAg                                            >  2:710255/1‑140 (MQ=255)                                                                                            gacgCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCTACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCNGCAGCTc                                         <  2:36140/140‑1 (MQ=255)                                                                                                 ccATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTg                                    <  2:319874/140‑1 (MQ=255)                                                                                                               ttAGCCTGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGgcagc                      <  1:277715/140‑1 (MQ=255)                                                                                                                     tGACTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGGCAGCAGGTTg                <  1:245183/140‑1 (MQ=255)                                                                                                                       aCTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGGCAGCAGGTTGAt              >  2:53454/1‑140 (MQ=255)                                                                                                                        cTCATAATTTTTCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGGCAGCAGGTTGATc             <  1:752315/140‑1 (MQ=255)                                                                                                                                   tCCCTTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGGCAGCAGGTTGATCCCTTCTTCCag  <  1:754498/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTGAGATTGTCCCGACTTCGGCAAGCTTTGTCGGCAGCATGATGATACCGGAGCCCATCATGTTGACCATCGTCAGTATGGTTAACTGAACGACGCCCATTTTGTTCGATTTAGCCTGACTCATAATTTTTCCCCTTTCAACAGGGTGCTTTGCGCATCACGAGGCTTGATGACATAGCACCAAACTTGCTTACGACCATCATGTTCTTCGATATAGACACCCTGCAGCTCCGGTGCAAAACCTGGCAGCAGGTTGATCCCTTCTTCCAG  >  NC_000913/718142‑718411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑