Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	783,505	G→A	A114T (GCG→ACG)	pnuC →	nicotinamide riboside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	783,505	0	G	A	100.0%	207.7 / NA	59	A114T (GCG→ACG)	pnuC	nicotinamide riboside transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/32);  total (27/32)

ATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTT  >  NC_000913/783368‑783640                                                                                                                                          |                                                                                                                                        aTGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACg                                                                                                                                       >  2:607187/1‑140 (MQ=255) aTGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACg                                                                                                                                       >  2:493894/1‑140 (MQ=255)     cAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGttt                                                                                                                                   >  2:769725/1‑140 (MQ=255)          tGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCgtt                                                                                                                              >  1:679575/1‑140 (MQ=255)            cTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCgttgt                                                                                                                            <  2:768090/140‑1 (MQ=255)              aTTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGttt                                                                                                                          <  1:769725/140‑1 (MQ=255)                  cAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGAt                                                                                                                      <  1:723110/140‑1 (MQ=255)                   aGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACAAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGAtt                                                                                                                     <  1:568650/140‑1 (MQ=255)                      tgtTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCg                                                                                                                                >  1:83335/1‑126 (MQ=255)                      tgtTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCg                                                                                                                                <  2:83335/126‑1 (MQ=255)                      tgtTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGt                                                                                                                  >  2:706369/1‑140 (MQ=255)                        tttttCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCt                                                                                                                >  2:681631/1‑140 (MQ=255)                           ttCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCtgat                                                                                                             >  1:102856/1‑140 (MQ=255)                           ttCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCtgat                                                                                                             <  1:622597/140‑1 (MQ=255)                             cTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCtgatga                                                                                                           <  1:97729/140‑1 (MQ=255)                             cTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCtgatga                                                                                                           <  1:719362/140‑1 (MQ=255)                                  ccGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTa                                                                                                      <  2:484111/140‑1 (MQ=255)                                     cgAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTAttt                                                                                                   >  2:253161/1‑140 (MQ=255)                                                ggTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGgt                                                                                        <  1:253161/140‑1 (MQ=255)                                                  ttGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATAGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGgtgt                                                                                      >  1:708584/1‑140 (MQ=255)                                                    ggTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAGATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGttt                                                                                    >  1:512931/1‑140 (MQ=255)                                                     gTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTg                                                                                   <  2:708584/140‑1 (MQ=255)                                                      tATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGc                                                                                  >  1:734322/1‑140 (MQ=255)                                                          cGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttt                                                                              <  2:679575/140‑1 (MQ=255)                                                            tGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAATGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttt                                                                            >  1:264588/1‑140 (MQ=255)                                                            tGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttt                                                                            >  1:704766/1‑140 (MQ=255)                                                              gTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGa                                                                          >  2:599373/1‑140 (MQ=255)                                                              gTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGa                                                                          >  1:721810/1‑140 (MQ=255)                                                               tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAc                                                                         >  2:654757/1‑140 (MQ=255)                                                               tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAc                                                                         <  2:561456/140‑1 (MQ=255)                                                               tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAc                                                                         <  2:721810/140‑1 (MQ=255)                                                               tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAc                                                                         <  2:734322/140‑1 (MQ=255)                                                               tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAc                                                                         >  2:208077/1‑140 (MQ=255)                                                               tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAc                                                                         <  1:320666/140‑1 (MQ=255)                                                                    aCAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCcgcg                                                                    >  1:383662/1‑140 (MQ=255)                                                                    aCAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCcgcg                                                                    <  1:213195/140‑1 (MQ=255)                                                                      aaaCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTg                                                                  <  1:91426/140‑1 (MQ=255)                                                                      aaaCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTg                                                                  <  1:599373/140‑1 (MQ=255)                                                                       aaCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTgg                                                                 <  2:383662/140‑1 (MQ=255)                                                                       aaCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTgg                                                                 <  1:574392/140‑1 (MQ=255)                                                                       aaCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTgg                                                                 <  1:292624/140‑1 (MQ=255)                                                                        aCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGc                                                                >  1:558892/1‑140 (MQ=255)                                                                             tCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCa                                                           <  1:654757/140‑1 (MQ=255)                                                                                      ggCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGc                                                  <  2:771615/140‑1 (MQ=255)                                                                                      ggCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGc                                                  <  2:587336/140‑1 (MQ=255)                                                                                             ttGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCAtt                                           <  2:704766/140‑1 (MQ=255)                                                                                              tGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTa                                          <  1:166361/140‑1 (MQ=255)                                                                                                     tCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTAc                                   <  1:607187/140‑1 (MQ=255)                                                                                                     tCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTAc                                   >  2:405739/1‑140 (MQ=255)                                                                                                        cTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAgg                                <  2:495183/140‑1 (MQ=255)                                                                                                                     ccGAAGGCACTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGa                   >  2:117355/1‑140 (MQ=255)                                                                                                                              cTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAAcc          <  1:706369/140‑1 (MQ=255)                                                                                                                              cTCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAAcc          >  1:53326/1‑140 (MQ=255)                                                                                                                               tCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTg                    <  1:283810/129‑1 (MQ=255)                                                                                                                               tCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTg                    >  2:283810/1‑129 (MQ=255)                                                                                                                               tCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCg         >  1:377308/1‑140 (MQ=255)                                                                                                                               tCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGCGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCta                    >  1:225317/1‑128 (MQ=255)                                                                                                                               tCAGCTGGTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGCGTTTGCATTTTTGACCCGCGTGGCAGTCATAATCATGCAAGCATTAGGATTACAGGTTGTGATGCCta                    <  2:225317/129‑2 (MQ=255)                                                                                                                                      gTTGACGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCtt  >  2:108324/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTT  >  NC_000913/783368‑783640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑