Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,701	C→T	intergenic (+81/+110)	nhaR → / ← insB‑1	DNA‑binding transcriptional activator NhaR/IS1 protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,701	0	C	T	100.0%	163.9 / NA	47	intergenic (+81/+110)	nhaR/insB‑1	DNA‑binding transcriptional activator NhaR/IS1 protein InsB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

TACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATAGTGTTTTA  >  NC_000913/19565‑19827                                                                                                                                         |                                                                                                                               tACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGc                                                                                                                             <  1:337798/140‑1 (MQ=255)      cGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTgag                                                                                                                        >  1:120812/1‑140 (MQ=255)      cGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTgag                                                                                                                        >  1:679714/1‑140 (MQ=255)         aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACa                                                                                                                     <  2:120812/140‑1 (MQ=255)         aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACa                                                                                                                     <  2:679714/140‑1 (MQ=255)                aTACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTc                                                                                                              >  1:47616/1‑140 (MQ=255)                aTACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTc                                                                                                              <  2:578381/140‑1 (MQ=255)                 tACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTcc                                                                                                             >  2:155785/1‑140 (MQ=255)                   cGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCat                                                                                                           >  2:224368/1‑140 (MQ=255)                      attattCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATg                                                                                                        >  1:667616/1‑140 (MQ=255)                      attattCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATg                                                                                                        >  1:628294/1‑140 (MQ=255)                            cTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTAcgcg                                                                                                  <  1:138479/140‑1 (MQ=255)                              gcgcTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCggg                                                                                                <  1:354910/140‑1 (MQ=255)                               cgcTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGc                                                                                               >  2:804882/1‑140 (MQ=255)                                 cTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGa                                                                                             <  1:66126/140‑1 (MQ=255)                                            aGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAgg                                                                                  <  1:413297/140‑1 (MQ=255)                                            aGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAgg                                                                                  >  2:408515/1‑140 (MQ=255)                                              cGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGaa                                                                                >  2:376061/1‑140 (MQ=255)                                                        aTCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTAct                                                                      >  1:701134/1‑140 (MQ=255)                                                          cGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTActtc                                                                    <  1:804882/140‑1 (MQ=255)                                                             cAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTActtcctt                                                                 >  1:325393/1‑140 (MQ=255)                                                                  cccccAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATgg                                                            <  2:628294/140‑1 (MQ=255)                                                                    cccAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTCCGCGGGCGAATAAATAGAGGAATCTGGTTACTTCCTTCATgggg                                                          <  2:503649/140‑1 (MQ=255)                                                                    cccAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATgggg                                                          <  2:667616/140‑1 (MQ=255)                                                                             aaGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGaaa                                                 >  2:242885/1‑140 (MQ=255)                                                                              aGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGaaaa                                                <  1:624034/140‑1 (MQ=255)                                                                                   ggggAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATgg                                                            >  2:809563/1‑123 (MQ=255)                                                                                   ggggAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATgg                                                            <  1:809563/123‑1 (MQ=255)                                                                                          tagatTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTg                                    <  1:458205/140‑1 (MQ=255)                                                                                                   tGTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATg                           >  1:554929/1‑140 (MQ=255)                                                                                                    gTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGa                          >  2:68142/1‑140 (MQ=255)                                                                                                    gTACATTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGa                          >  2:492797/1‑140 (MQ=255)                                                                                                        aTTACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTcc                      <  1:285120/140‑1 (MQ=255)                                                                                                          tACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCaa                    >  2:407403/1‑140 (MQ=255)                                                                                                          tACCACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCaa                    >  2:663966/1‑140 (MQ=255)                                                                                                            ccACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACt                  >  1:634310/1‑140 (MQ=255)                                                                                                            ccACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACt                  >  2:657423/1‑140 (MQ=255)                                                                                                            ccACGATTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACt                  >  2:409358/1‑140 (MQ=255)                                                                                                                 aTTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTg             >  2:221715/1‑140 (MQ=255)                                                                                                                 aTTTTGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTg             <  1:224368/140‑1 (MQ=255)                                                                                                                  ttttGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGa            <  2:554929/140‑1 (MQ=255)                                                                                                                   tttGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAt           <  2:576720/140‑1 (MQ=255)                                                                                                                   tttGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAt           <  2:452273/140‑1 (MQ=255)                                                                                                                   tttGACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGAt           <  2:716649/140‑1 (MQ=255)                                                                                                                      gACTCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATAgt        >  2:505423/1‑140 (MQ=255)                                                                                                                         tCGGCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATAGTGtt     <  2:634310/140‑1 (MQ=255)                                                                                                                           ggCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATAGTGtttt   <  1:68142/140‑1 (MQ=255)                                                                                                                            gCTCATTATTTGCTCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATAGTGTTTTa  >  1:611633/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               TACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATAGTGTTTTA  >  NC_000913/19565‑19827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑