Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	805,840	G→A	intergenic (+76/+158)	ybhJ → / ← ybhC	putative hydratase YbhJ/outer membrane lipoprotein YhbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	805,840	0	G	A	100.0%	24.3 / NA	13	intergenic (+76/+158)	ybhJ/ybhC	putative hydratase YbhJ/outer membrane lipoprotein YhbC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

CGCGAGATTATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATT  >  NC_000913/805708‑805896                                                                                                                                     |                                                         cgcgAGATTATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCa                                                   >  1:423966/1‑140 (MQ=255)    gagaTTATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCg                                                <  1:288665/140‑1 (MQ=255)         tATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGaaccg                                           <  1:167247/140‑4 (MQ=255)                   ggTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGAt                                <  2:644647/140‑1 (MQ=21)                    gTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATa                               >  1:199072/1‑140 (MQ=21)                    gTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATa                               >  1:302300/1‑140 (MQ=21)                     tAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATaa                              >  2:89188/1‑140 (MQ=21)                                  ttCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACgccg                 >  2:343902/1‑138 (MQ=17)                                          aaaCCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc         <  2:392324/140‑1 (MQ=14)                                          aaaCCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc         <  2:522708/140‑1 (MQ=14)                                            aCCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa       >  1:264326/1‑139 (MQ=14)                                                 cAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaaatagt  >  2:601825/1‑134 (MQ=11)                                                        aaaaaGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATcc            <  1:787331/123‑1 (MQ=11)                                                        aaaaaGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAA‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATcc            >  2:787331/1‑123 (MQ=11)                                                                                                                                     |                                                         CGCGAGATTATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATT  >  NC_000913/805708‑805896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑