Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,010,898	C→T	A312V (GCC→GTC)	uup →	ATP‑binding protein with possible role in replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,010,898	0	C	T	100.0%	113.6 / NA	34	A312V (GCC→GTC)	uup	ATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

AGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGT  >  NC_000913/1010761‑1011029                                                                                                                                          |                                                                                                                                    agagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCa                                                                                                                                   >  1:289066/1‑140 (MQ=255)        tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTc                                                                                                                            <  1:228819/140‑1 (MQ=255)                ccAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTaaaa                                                                                                                    >  1:140862/1‑140 (MQ=255)                       aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGtttt                                                                                                             >  1:781495/1‑140 (MQ=255)                       aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGtttt                                                                                                             <  1:57112/140‑1 (MQ=255)                       aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGACGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGtttt                                                                                                             >  1:648593/1‑140 (MQ=255)                                cGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGa                                                                                                    >  1:50082/1‑140 (MQ=255)                                 gCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGaa                                                                                                   >  2:103646/1‑140 (MQ=255)                                 gCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGaa                                                                                                   >  1:422130/1‑140 (MQ=255)                                   cGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGa                                                                                                 <  1:30007/140‑1 (MQ=255)                                    gTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGAc                                                                                                >  2:698077/1‑140 (MQ=255)                                      aCCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt                                                                                              >  1:199667/1‑140 (MQ=255)                                             aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                       <  2:140862/140‑1 (MQ=255)                                             aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                       <  1:560152/140‑1 (MQ=255)                                             aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                       <  2:609438/140‑1 (MQ=255)                                             aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                       >  1:339223/1‑140 (MQ=255)                                                  ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                                  >  1:570282/1‑140 (MQ=255)                                                  ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                                  <  2:339223/140‑1 (MQ=255)                                                   gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGtt                                                                                 <  2:289066/140‑1 (MQ=255)                                                       cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACg                                                                             >  1:233641/1‑140 (MQ=255)                                                                     aaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTg                                                               <  2:781495/140‑1 (MQ=255)                                                                          aTGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGa                                                          >  2:125958/1‑140 (MQ=255)                                                                                    aaCGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                <  2:199667/140‑1 (MQ=255)                                                                                    aaCGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                <  1:434411/140‑1 (MQ=255)                                                                                     aCGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGccc                                               <  1:125958/140‑1 (MQ=255)                                                                                                gTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGt                                    <  1:698077/140‑1 (MQ=255)                                                                                                  cgcgAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                <  2:234295/126‑1 (MQ=255)                                                                                                  cgcgAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                >  1:234295/1‑126 (MQ=255)                                                                                                               gTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGcc                     <  2:422130/140‑1 (MQ=255)                                                                                                                tACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGccc                    >  2:686185/1‑140 (MQ=255)                                                                                                                 aCCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCt                   >  2:409925/1‑140 (MQ=255)                                                                                                                   cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                 >  2:432336/1‑140 (MQ=255)                                                                                                                                      gagGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGt  >  1:655986/1‑136 (MQ=255)                                                                                                                                      gagGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGt  <  2:655986/136‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    AGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGT  >  NC_000913/1010761‑1011029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑