Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,068,636	T→G	T402P (ACG→CCG)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,636	0	T	G	100.0%	96.5 / NA	28	T402P (ACG→CCG)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/14);  total (14/14)

CGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACA  >  NC_000913/1068500‑1068763                                                                                                                                         |                                                                                                                                cggTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGgag                                                                                                                              >  2:411445/1‑140 (MQ=255)                ggCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCcgc                                                                                                               >  1:348919/1‑140 (MQ=255)                ggCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCcgc                                                                                                               >  2:697314/1‑140 (MQ=255)                   tCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGcc                                                                                                            <  1:411445/140‑1 (MQ=255)                   tCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGcc                                                                                                            <  1:84837/140‑1 (MQ=255)                             caacTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAg                                                                                                  >  1:150129/1‑140 (MQ=255)                             caacTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAg                                                                                                  <  1:595681/140‑1 (MQ=255)                                     ggCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGc                                                                                          >  1:656259/1‑140 (MQ=255)                                          tGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCa                                                                                     >  1:787250/1‑140 (MQ=255)                                           ggAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCAt                                                                                    <  2:673743/140‑1 (MQ=255)                                                                           aTTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGacac                                                    <  2:150129/140‑1 (MQ=255)                                                                            ttGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACg                                                   <  1:697314/140‑1 (MQ=255)                                                                               aGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtt                                                >  1:626603/1‑140 (MQ=255)                                                                                 tAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtttt                                              <  2:348919/140‑1 (MQ=255)                                                                                  aaaaTCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTg                                             <  2:656259/140‑1 (MQ=255)                                                                                     aTCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAc                                          >  1:704154/1‑140 (MQ=255)                                                                                         cgcCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCat                                      >  1:27375/1‑140 (MQ=255)                                                                                                      tGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTg                         <  2:27375/140‑1 (MQ=255)                                                                                                      tGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTg                         >  1:245190/1‑140 (MQ=255)                                                                                                          ccAATCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGAc                     >  1:406765/1‑140 (MQ=255)                                                                                                             aTCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGc                  >  2:142157/1‑140 (MQ=255)                                                                                                             aTCCCTCCCAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGc                  >  2:495405/1‑140 (MQ=255)                                                                                                                  tcccAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCa             >  1:734737/1‑140 (MQ=255)                                                                                                                    ccAACGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGc           <  1:53854/140‑1 (MQ=255)                                                                                                                       aCGTAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCg        <  2:83682/140‑1 (MQ=255)                                                                                                                          tAAAACCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac     <  1:775198/140‑1 (MQ=255)                                                                                                                            aaaCCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAacac   <  1:299238/140‑1 (MQ=255)                                                                                                                             aaCCGCCCAGCGGGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAAcaca  <  2:787250/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                CGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACA  >  NC_000913/1068500‑1068763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑