Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,491	T→C	T151A (ACG→GCG)	opgC ←	protein required for succinyl modification of osmoregulated periplasmic glucans

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,491	0	T	C	100.0%	111.6 / NA	32	T151A (ACG→GCG)	opgC	protein required for succinyl modification of osmoregulated periplasmic glucans
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

ATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTG  >  NC_000913/1108364‑1108626                                                                                                                                |                                                                                                                                        attaCCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCAc                                                                                                                             >  2:791073/1‑140 (MQ=255)      cGCATAACCGATGCCGAGGCAT‑AAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAacac                                                                                                                       <  1:472141/140‑1 (MQ=255)        cATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACAcc                                                                                                                      >  2:433598/1‑140 (MQ=255)        cATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACAcc                                                                                                                      >  1:768591/1‑140 (MQ=255)               gATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTaaa                                                                                                                >  1:341550/1‑139 (MQ=255)               gATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTaaa                                                                                                                <  2:341550/139‑1 (MQ=255)                aTGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTaaaaa                                                                                                              >  2:118005/1‑140 (MQ=255)                  gCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAAcc                                                                                                            >  1:778124/1‑140 (MQ=255)                    cGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACcac                                                                                                          <  1:19559/140‑1 (MQ=255)                            aaaaaaaTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGa                                                                                                  <  2:778124/140‑1 (MQ=255)                                  aTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTaa                                                                                            >  2:441051/1‑140 (MQ=255)                                           aGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGc                                                                                   <  1:413327/140‑1 (MQ=255)                                            gTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGcc                                                                                  <  2:458456/140‑1 (MQ=255)                                             ttttACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCa                                                                                 <  2:199891/140‑1 (MQ=255)                                                     tCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGtatta                                                                         >  1:223670/1‑140 (MQ=255)                                                                 tATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATAc                                                             <  2:411083/140‑1 (MQ=255)                                                                 tATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATAc                                                             <  2:128317/140‑1 (MQ=255)                                                                                aaTTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGc                                              <  1:791073/140‑1 (MQ=255)                                                                                     tCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACt                                         >  1:445666/1‑140 (MQ=255)                                                                                          attattTCTGATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCg                                    >  1:16857/1‑140 (MQ=255)                                                                                                   gATGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCtt                           <  2:445666/140‑1 (MQ=255)                                                                                                    aTGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCttt                          >  1:609215/1‑140 (MQ=255)                                                                                                     tGCGCTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTg                         >  2:441687/1‑140 (MQ=255)                                                                                                       cgcTTAAATATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGAc                       <  1:118005/140‑1 (MQ=255)                                                                                                             aaTATCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTg                 <  1:115427/140‑1 (MQ=255)                                                                                                               tatCCATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTgca               <  1:248721/140‑1 (MQ=255)                                                                                                                  ccATACGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTgcagca            >  1:518609/1‑140 (MQ=255)                                                                                                                      aCGCACAGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCataat        >  1:745173/1‑140 (MQ=255)                                                                                                                          acaGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTg                 <  2:376754/127‑1 (MQ=255)                                                                                                                          acaGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTg                 >  1:376754/1‑127 (MQ=255)                                                                                                                          acaGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAAt    >  2:619709/1‑140 (MQ=255)                                                                                                                            aGCGCCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTg  >  2:748859/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                        ATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTG  >  NC_000913/1108364‑1108626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑