Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,124,696	C→T	V211I (GTC→ATC)	mdtH ←	multidrug efflux pump MdtH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,124,696	0	C	T	100.0%	143.8 / NA	41	V211I (GTC→ATC)	mdtH	multidrug efflux pump MdtH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/18);  total (23/18)

ACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGC  >  NC_000913/1124561‑1124827                                                                                                                                        |                                                                                                                                    acagacaCGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATaaaa                                                                                                                                 >  1:692968/1‑140 (MQ=255)    gacaCGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGc                                                                                                                              >  2:784552/1‑140 (MQ=255)            tCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACg                                                                                                                      >  2:614904/1‑140 (MQ=255)            tCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACg                                                                                                                      >  2:533678/1‑140 (MQ=255)                   cATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCAcg                                                                                                               <  1:334272/140‑1 (MQ=255)                   cATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCAcg                                                                                                               <  1:614904/140‑1 (MQ=255)                   cATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCAcg                                                                                                               <  2:439878/140‑1 (MQ=255)                   cATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCAcg                                                                                                               >  1:536336/1‑140 (MQ=255)                     tgcATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCAcgcg                                                                                                             >  1:262560/3‑140 (MQ=255)                       cATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCggg                                                                                                           >  1:207857/1‑140 (MQ=255)                                          ggggcgcCAGCCACGTCGTTTACAATAATTGGCAGCATCGGCACCACTTGTACAGCCCGCATATAGTACCCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACggg                                                                                        <  2:144875/137‑1 (MQ=255)                                          ggCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACggg                                                                                        <  1:532637/140‑1 (MQ=255)                                              cgcCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGgcgtg                                                                                    >  1:432294/1‑140 (MQ=255)                                               cccagccaCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGgcgtgc                                                                                   <  2:645146/139‑1 (MQ=255)                                                 cagccaCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGgcgtgcgt                                                                                 <  2:692968/140‑1 (MQ=255)                                                    ccaCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACg                                                                              >  2:198840/1‑140 (MQ=255)                                                          cgtTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGgag                                                                        <  2:432294/140‑1 (MQ=255)                                                           gtTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGgaga                                                                       >  1:381723/1‑140 (MQ=255)                                                               aCCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGttt                                                                   <  2:536336/140‑1 (MQ=255)                                                                    aaTTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATg                                                              <  1:784552/140‑1 (MQ=255)                                                                    aaTTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATg                                                              <  1:194582/140‑1 (MQ=255)                                                                    aaTTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATg                                                              >  2:194582/1‑140 (MQ=255)                                                                         gcagcaTCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGt                                                         >  1:493344/1‑140 (MQ=255)                                                                           agcatcagcatcACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTaa                                                       >  2:134831/1‑140 (MQ=255)                                                                                cagcatcaCTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAAcc                                                  <  1:533678/140‑1 (MQ=255)                                                                                         ttGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGa                                         <  1:420169/140‑1 (MQ=255)                                                                                             aCAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAAcgc                                     >  1:443460/1‑140 (MQ=255)                                                                                              cagccagcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAAcgcc                                    <  2:207857/140‑1 (MQ=255)                                                                                                 ccagcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGca                                 >  1:430380/1‑140 (MQ=255)                                                                                                   agcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGcaca                               >  1:426251/1‑140 (MQ=255)                                                                                                     cATGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATa                             >  2:540137/1‑140 (MQ=255)                                                                                                       tGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc                           <  1:540137/140‑1 (MQ=255)                                                                                                       tGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc                           <  2:623884/140‑1 (MQ=255)                                                                                                       tGTAGTAACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc                           <  1:198840/140‑1 (MQ=255)                                                                                                          agtaACCCGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGcaca                        >  2:57853/1‑140 (MQ=255)                                                                                                                 cGCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGaa                 >  1:584127/1‑140 (MQ=255)                                                                                                                  gCCAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAAc                <  2:584127/140‑1 (MQ=255)                                                                                                                   ccAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc                           <  1:612324/128‑1 (MQ=255)                                                                                                                   ccAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc                           >  2:612324/1‑128 (MQ=255)                                                                                                                   ccAGCGTCAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACt               >  2:448140/1‑140 (MQ=255)                                                                                                                          cAGAACATAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCtg        >  2:509911/1‑140 (MQ=255)                                                                                                                                aTAGGTGATAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGgcgc  >  2:267206/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    ACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGC  >  NC_000913/1124561‑1124827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑