Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,224,789	A→G	G191G (GGT→GGC)	minD ←	Z‑ring positioning protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,789	0	A	G	100.0%	107.7 / NA	31	G191G (GGT→GGC)	minD	Z‑ring positioning protein MinD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/11);  total (20/11)

GTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGA  >  NC_000913/1224666‑1224922                                                                                                                            |                                                                                                                                      gTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGc                                                                                                                       <  1:141253/140‑1 (MQ=255)     aTGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTgg                                                                                                                   >  2:282754/1‑140 (MQ=255)               tGACCGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAgcgc                                                                                                         >  1:500045/1‑140 (MQ=255)                gACCGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGcgcg                                                                                                        <  1:418814/140‑1 (MQ=255)                          cccTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCCGTCTGGGTTATAGCGCGTTAAcagcag                                                                                              <  2:432330/140‑1 (MQ=255)                           ccTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAgg                                                                                             <  2:215178/140‑1 (MQ=255)                              ggTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGc                                                                                          >  1:557104/1‑140 (MQ=255)                               gTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGct                                                                                         <  1:26676/140‑1 (MQ=255)                                         cgcAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAgg                                                                                             >  2:57668/1‑126 (MQ=255)                                         cgcAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAgg                                                                                             <  1:57668/126‑1 (MQ=255)                                         cgcAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAgg                                                                               >  2:299731/1‑140 (MQ=255)                                           cAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGct                                                                             <  2:683099/140‑1 (MQ=255)                                                        tCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTc                                                                >  1:688672/1‑140 (MQ=255)                                                        tCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTc                                                                >  2:174463/1‑140 (MQ=255)                                                        tCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTc                                                                >  2:119029/1‑140 (MQ=255)                                                                gATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCgcggc                                                        <  1:564964/140‑1 (MQ=255)                                                                      gCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGAtt                                                  >  1:193694/1‑140 (MQ=255)                                                                                 tGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGa                                       <  1:282754/140‑1 (MQ=255)                                                                                 tGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGa                                       <  2:193694/140‑1 (MQ=255)                                                                                        cAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTa                                >  2:351006/1‑140 (MQ=255)                                                                                        cAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTa                                >  1:476375/1‑140 (MQ=255)                                                                                         aGGATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTaa                               >  2:256865/1‑140 (MQ=255)                                                                                           gATCTCCAGCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTCTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTaaaa                             >  2:266752/1‑140 (MQ=255)                                                                                                   gCACATCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCa                     <  2:557104/140‑1 (MQ=255)                                                                                                    cacaTCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCag                    >  1:484996/1‑140 (MQ=255)                                                                                                     acaTCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCaga                   >  1:697446/1‑140 (MQ=255)                                                                                                        tCTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTc                <  1:511753/140‑1 (MQ=255)                                                                                                         cTTCCATGCTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTcg               >  1:421788/1‑140 (MQ=255)                                                                                                                 cTCAGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTga       >  2:488797/1‑140 (MQ=255)                                                                                                                    aGCATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTgagga    >  1:174414/1‑140 (MQ=255)                                                                                                                      cATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGgaga  >  1:591971/1‑140 (MQ=255)                                                                                                                      cATGTCGCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGgaga  >  1:371166/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                      GTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGA  >  NC_000913/1224666‑1224922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑